Abnormal proteins enhance stress-induced cell death

Se Jin Kim, Sunghoon Kim

Research output: Contribution to journalArticle

Abstract

The effect of abnormal proteins on cell viability was studied using artificially cleaved polypeptides. Escherichia coli methionyl-tRNA synthetase (MetRS) consists of two distinct domains and its activity is essential for cell viability. The polypeptide chain was split by linker insertion and expressed as two fragments. Two pairs of polypeptides, one split within the N-terminal domain and another at the junction of the two domains retained aminoacylation activity. The in vitro activities of these split mutants were enhanced by the presence of chaperonin, GroESL. However, cells containing these split polypeptides became sensitive to conditions that induce GroESL. The results of this work suggest that an abnormally generated protein can cause cell death under stressful conditions.

Original languageEnglish
Pages (from-to)153-157
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume243
Issue number1
DOIs
Publication statusPublished - 1998 Feb 4

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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