Acetylation of cyclin-dependent kinase 5 is mediated by GCN5

Juhyung Lee, Nuri Yun, Chiho Kim, Min Young Song, Kang Sik Park, Young Jun Oh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cyclin-dependent kinase 5 (CDK5), a member of atypical serine/threonine cyclin-dependent kinase family, plays a crucial role in pathophysiology of neurodegenerative disorders. Its kinase activity and substrate specificity are regulated by several independent pathways including binding with its activator, phosphorylation and S-nitrosylation. In the present study, we report that acetylation of CDK5 comprises an additional posttranslational modification within the cells. Among many candidates, we confirmed that its acetylation is enhanced by GCN5, a member of the GCN5-related N-acetyl-transferase family of histone acetyltransferase. Co-immunoprecipitation assay and fluorescent localization study indicated that GCN5 physically interacts with CDK5 and they are co-localized at the specific nuclear foci. Furthermore, liquid chromatography in conjunction with a mass spectrometry indicated that CDK5 is acetylated at Lys33 residue of ATP binding domain. Considering this lysine site is conserved among a wide range of species and other related cyclin-dependent kinases, therefore, we speculate that acetylation may alter the kinase activity of CDK5 via affecting efficacy of ATP coordination.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume447
Issue number1
DOIs
Publication statusPublished - 2014 Apr 25

Fingerprint

Cyclin-Dependent Kinase 5
Acetylation
Cyclin-Dependent Kinases
Phosphotransferases
Adenosine Triphosphate
Histone Acetyltransferases
Phosphorylation
Protein-Serine-Threonine Kinases
Liquid chromatography
Threonine
Post Translational Protein Processing
Transferases
Substrate Specificity
Immunoprecipitation
Liquid Chromatography
Neurodegenerative Diseases
Serine
Lysine
Mass spectrometry
Assays

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Lee, Juhyung ; Yun, Nuri ; Kim, Chiho ; Song, Min Young ; Park, Kang Sik ; Oh, Young Jun. / Acetylation of cyclin-dependent kinase 5 is mediated by GCN5. In: Biochemical and Biophysical Research Communications. 2014 ; Vol. 447, No. 1. pp. 121-127.
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Acetylation of cyclin-dependent kinase 5 is mediated by GCN5. / Lee, Juhyung; Yun, Nuri; Kim, Chiho; Song, Min Young; Park, Kang Sik; Oh, Young Jun.

In: Biochemical and Biophysical Research Communications, Vol. 447, No. 1, 25.04.2014, p. 121-127.

Research output: Contribution to journalArticle

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