Acidification induces OGR1/Ca 2+ /calpain signaling in gingival fibroblasts

Mi Seong Kim, Dong Min Shin, Min Seuk Kim

Research output: Contribution to journalArticle

Abstract

Gingivitis, the mildest form of periodontitis, is generally considered a consequence of prolonged exposure of the gingiva to periodontal pathogens. On the other hand, several epidemiologic reports have suggested that other etiologic factors such as oral acidification may also increase the susceptibility of the periodontium to destruction. However, the pathologic mechanism underlying the effects of oral acidification on the gingiva is still largely unknown. In this study, we analyzed molecular pathways mediating the influence of the acidic environment on human gingival fibroblasts (HGFs). Acidic extracellular pH caused biphasic increase of intracellular Ca 2+ level ([Ca 2+ ] i ) through activation of ovarian cancer G protein-coupled receptor 1, phospholipase C, and Ca 2+ release from the endoplasmic reticulum, but not through voltage-gated Ca 2+ channels or extracellular Ca 2+ influx via transient receptor potential cation channel subfamily V member 1. The acidic environment was also transiently cytotoxic for HGFs; however, the activation of pro-apoptotic proteins poly (ADP-ribose) polymerase-1 and BAX was not observed. Furthermore, we found that intracellular matrix metalloproteinase 1 was consistently upregulated in HGFs grown in regular medium, but significantly reduced in the acidic medium, which depended on [Ca 2+ ] i increase, lysosomal pH homeostasis, and Ca 2+ -dependent protease calpain. Considering that HGFs, essential for oral wound healing, in the in vitro culture system are placed in wound repair-like conditions, our findings provide important insights into molecular mechanisms underlying HGF functional impairment and chronic damage to the gingiva caused by the acidic intraoral environment.

Original languageEnglish
Pages (from-to)693-699
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume496
Issue number2
DOIs
Publication statusPublished - 2018 Feb 5

Fingerprint

Calpain
Acidification
Fibroblasts
Gingiva
Chemical activation
Transient Receptor Potential Channels
Periodontium
Gingivitis
Matrix Metalloproteinase 1
Apoptosis Regulatory Proteins
Poly(ADP-ribose) Polymerases
Periodontitis
Type C Phospholipases
Pathogens
G-Protein-Coupled Receptors
Endoplasmic Reticulum
Ovarian Neoplasms
Wound Healing
Homeostasis
Repair

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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title = "Acidification induces OGR1/Ca 2+ /calpain signaling in gingival fibroblasts",
abstract = "Gingivitis, the mildest form of periodontitis, is generally considered a consequence of prolonged exposure of the gingiva to periodontal pathogens. On the other hand, several epidemiologic reports have suggested that other etiologic factors such as oral acidification may also increase the susceptibility of the periodontium to destruction. However, the pathologic mechanism underlying the effects of oral acidification on the gingiva is still largely unknown. In this study, we analyzed molecular pathways mediating the influence of the acidic environment on human gingival fibroblasts (HGFs). Acidic extracellular pH caused biphasic increase of intracellular Ca 2+ level ([Ca 2+ ] i ) through activation of ovarian cancer G protein-coupled receptor 1, phospholipase C, and Ca 2+ release from the endoplasmic reticulum, but not through voltage-gated Ca 2+ channels or extracellular Ca 2+ influx via transient receptor potential cation channel subfamily V member 1. The acidic environment was also transiently cytotoxic for HGFs; however, the activation of pro-apoptotic proteins poly (ADP-ribose) polymerase-1 and BAX was not observed. Furthermore, we found that intracellular matrix metalloproteinase 1 was consistently upregulated in HGFs grown in regular medium, but significantly reduced in the acidic medium, which depended on [Ca 2+ ] i increase, lysosomal pH homeostasis, and Ca 2+ -dependent protease calpain. Considering that HGFs, essential for oral wound healing, in the in vitro culture system are placed in wound repair-like conditions, our findings provide important insights into molecular mechanisms underlying HGF functional impairment and chronic damage to the gingiva caused by the acidic intraoral environment.",
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Acidification induces OGR1/Ca 2+ /calpain signaling in gingival fibroblasts . / Kim, Mi Seong; Shin, Dong Min; Kim, Min Seuk.

In: Biochemical and Biophysical Research Communications, Vol. 496, No. 2, 05.02.2018, p. 693-699.

Research output: Contribution to journalArticle

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