Acquisition of human alveolar bone-derived stromal cells using minimally irrigated implant osteotomy

In vitro and in vivo evaluations

Jung Chul Park, Jane C. Kim, Yong Tae Kim, Seong Ho Choi, Kyoo Sung Cho, Gun Il Im, Byung Soo Kim, Chang Sung Kim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objectives Implant osteotomy yields a substantial amount of bone in the form of bone chips entrapped within drill flutes, and can provide a promising cell source for tissue engineering. The aims of this study were to isolate human alveolar bone-derived stromal cells (hABCs) obtained during implant osteotomy, and to evaluate osteogenic differentiation capacity of hABCs. Material and methods Bone chips were obtained by minimally irrigated implant drilling technique from 10 human donors. Isolated cells were studied with respect to their colony-forming efficiency, surface marker expression by immunofluorescence staining, fluorescence-activated cell sorting analysis and self-renewal potency. To verify the differentiation activity, in vitro osteogenic and adipogenic gene expressions were evaluated by reverse transcription-polymerase chain reaction, and in vitro formation of mineralized nodule and adipocytes was also evaluated. In vivo bone-forming activity was assessed by ectopic transplantation in immunocompromised mice (n = 5). Results Human alveolar bone-derived stromal cells population with characteristics of mesenchymal stem cells was present in the isolated cells. Upon hABC transplantation, significant ectopic bone formation was induced with the characteristics of fully matured bone tissue. Conclusion The data support the feasibility of using hABCs as a source of stem cells for dentoalveolar bone tissue reconstruction. The cell source has an advantage that the hABCs can be easily acquired during implant surgery.

Original languageEnglish
Pages (from-to)495-505
Number of pages11
JournalJournal of Clinical Periodontology
Volume39
Issue number5
DOIs
Publication statusPublished - 2012 May 1

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Stromal Cells
Osteotomy
Bone and Bones
In Vitro Techniques
Mandrillus
Cell Transplantation
Population Characteristics
Tissue Engineering
Mesenchymal Stromal Cells
Adipocytes
Osteogenesis
Reverse Transcription
Fluorescent Antibody Technique
Flow Cytometry
Stem Cells
Transplantation
Staining and Labeling
Gene Expression
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

Park, Jung Chul ; Kim, Jane C. ; Kim, Yong Tae ; Choi, Seong Ho ; Cho, Kyoo Sung ; Im, Gun Il ; Kim, Byung Soo ; Kim, Chang Sung. / Acquisition of human alveolar bone-derived stromal cells using minimally irrigated implant osteotomy : In vitro and in vivo evaluations. In: Journal of Clinical Periodontology. 2012 ; Vol. 39, No. 5. pp. 495-505.
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Acquisition of human alveolar bone-derived stromal cells using minimally irrigated implant osteotomy : In vitro and in vivo evaluations. / Park, Jung Chul; Kim, Jane C.; Kim, Yong Tae; Choi, Seong Ho; Cho, Kyoo Sung; Im, Gun Il; Kim, Byung Soo; Kim, Chang Sung.

In: Journal of Clinical Periodontology, Vol. 39, No. 5, 01.05.2012, p. 495-505.

Research output: Contribution to journalArticle

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T1 - Acquisition of human alveolar bone-derived stromal cells using minimally irrigated implant osteotomy

T2 - In vitro and in vivo evaluations

AU - Park, Jung Chul

AU - Kim, Jane C.

AU - Kim, Yong Tae

AU - Choi, Seong Ho

AU - Cho, Kyoo Sung

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AU - Kim, Byung Soo

AU - Kim, Chang Sung

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N2 - Objectives Implant osteotomy yields a substantial amount of bone in the form of bone chips entrapped within drill flutes, and can provide a promising cell source for tissue engineering. The aims of this study were to isolate human alveolar bone-derived stromal cells (hABCs) obtained during implant osteotomy, and to evaluate osteogenic differentiation capacity of hABCs. Material and methods Bone chips were obtained by minimally irrigated implant drilling technique from 10 human donors. Isolated cells were studied with respect to their colony-forming efficiency, surface marker expression by immunofluorescence staining, fluorescence-activated cell sorting analysis and self-renewal potency. To verify the differentiation activity, in vitro osteogenic and adipogenic gene expressions were evaluated by reverse transcription-polymerase chain reaction, and in vitro formation of mineralized nodule and adipocytes was also evaluated. In vivo bone-forming activity was assessed by ectopic transplantation in immunocompromised mice (n = 5). Results Human alveolar bone-derived stromal cells population with characteristics of mesenchymal stem cells was present in the isolated cells. Upon hABC transplantation, significant ectopic bone formation was induced with the characteristics of fully matured bone tissue. Conclusion The data support the feasibility of using hABCs as a source of stem cells for dentoalveolar bone tissue reconstruction. The cell source has an advantage that the hABCs can be easily acquired during implant surgery.

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