Abstract
Background: Though acral lentiginous melanoma (ALM) is a major type of malignant melanoma, no immunohistochemical study on this type of melanoma has been reported. Objective: The purpose of this study is to analysis the immunohistochemical findings of ALM using routinely used immune markers. Methods: An immunohistochemical study was performed on paraffin sections of 20 ALMs using S-100 protein, HMB-45, MART-1, vimentin, epithelial membrane antigen (EMA) and CAM 5.2. Results: S-100 protein (95%) was found to be a more sensitive marker than either HMB-45 (80%) or MART-1 (70%) for recognizing ALM. Melanin bleaching was useful for recognizing heavily pigmented ALM using both S-100 protein and HMB-45. The intensity of HMB-45 correlated well with the melanin content. However, there was no significant correlation between the intensity of S-100 protein and the melanin content. One and two out of 20 cases stained focally with EMA and CAM5.2, respectively, but these cases stained also with HMB-45 and/or S-100 protein. Conclusions: S-100 protein and HMB-45 were relatively sensitive markers for recognizing ALM. Despite the occasional positivity for the epithelial markers in ALM, all epithelial marker-positive cases stained also with HMB-45 and/or S-100 protein. Therefore, we recommend that the panel of antibodies used for recognizing ALM should contain at least S-100 protein and HMB-45.
| Original language | English |
|---|---|
| Pages (from-to) | 123-129 |
| Number of pages | 7 |
| Journal | International Journal of Dermatology |
| Volume | 42 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2003 Feb 1 |
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All Science Journal Classification (ASJC) codes
- Dermatology
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Acral lentiginous melanoma : An immunohistochemical study of 20 cases. / Kim, You Chan; Lee, Mingeol; Choe, Sung Whan; Lee, Min Cheol; Chung, Han Gil; Cho, Sang Ho.
In: International Journal of Dermatology, Vol. 42, No. 2, 01.02.2003, p. 123-129.Research output: Contribution to journal › Article
TY - JOUR
T1 - Acral lentiginous melanoma
T2 - An immunohistochemical study of 20 cases
AU - Kim, You Chan
AU - Lee, Mingeol
AU - Choe, Sung Whan
AU - Lee, Min Cheol
AU - Chung, Han Gil
AU - Cho, Sang Ho
PY - 2003/2/1
Y1 - 2003/2/1
N2 - Background: Though acral lentiginous melanoma (ALM) is a major type of malignant melanoma, no immunohistochemical study on this type of melanoma has been reported. Objective: The purpose of this study is to analysis the immunohistochemical findings of ALM using routinely used immune markers. Methods: An immunohistochemical study was performed on paraffin sections of 20 ALMs using S-100 protein, HMB-45, MART-1, vimentin, epithelial membrane antigen (EMA) and CAM 5.2. Results: S-100 protein (95%) was found to be a more sensitive marker than either HMB-45 (80%) or MART-1 (70%) for recognizing ALM. Melanin bleaching was useful for recognizing heavily pigmented ALM using both S-100 protein and HMB-45. The intensity of HMB-45 correlated well with the melanin content. However, there was no significant correlation between the intensity of S-100 protein and the melanin content. One and two out of 20 cases stained focally with EMA and CAM5.2, respectively, but these cases stained also with HMB-45 and/or S-100 protein. Conclusions: S-100 protein and HMB-45 were relatively sensitive markers for recognizing ALM. Despite the occasional positivity for the epithelial markers in ALM, all epithelial marker-positive cases stained also with HMB-45 and/or S-100 protein. Therefore, we recommend that the panel of antibodies used for recognizing ALM should contain at least S-100 protein and HMB-45.
AB - Background: Though acral lentiginous melanoma (ALM) is a major type of malignant melanoma, no immunohistochemical study on this type of melanoma has been reported. Objective: The purpose of this study is to analysis the immunohistochemical findings of ALM using routinely used immune markers. Methods: An immunohistochemical study was performed on paraffin sections of 20 ALMs using S-100 protein, HMB-45, MART-1, vimentin, epithelial membrane antigen (EMA) and CAM 5.2. Results: S-100 protein (95%) was found to be a more sensitive marker than either HMB-45 (80%) or MART-1 (70%) for recognizing ALM. Melanin bleaching was useful for recognizing heavily pigmented ALM using both S-100 protein and HMB-45. The intensity of HMB-45 correlated well with the melanin content. However, there was no significant correlation between the intensity of S-100 protein and the melanin content. One and two out of 20 cases stained focally with EMA and CAM5.2, respectively, but these cases stained also with HMB-45 and/or S-100 protein. Conclusions: S-100 protein and HMB-45 were relatively sensitive markers for recognizing ALM. Despite the occasional positivity for the epithelial markers in ALM, all epithelial marker-positive cases stained also with HMB-45 and/or S-100 protein. Therefore, we recommend that the panel of antibodies used for recognizing ALM should contain at least S-100 protein and HMB-45.
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UR - http://www.scopus.com/inward/citedby.url?scp=0038203473&partnerID=8YFLogxK
U2 - 10.1046/j.1365-4362.2003.01583.x
DO - 10.1046/j.1365-4362.2003.01583.x
M3 - Article
C2 - 12709000
AN - SCOPUS:0038203473
VL - 42
SP - 123
EP - 129
JO - International Journal of Dermatology
JF - International Journal of Dermatology
SN - 0011-9059
IS - 2
ER -