Acral lentiginous melanoma: An immunohistochemical study of 20 cases

You Chan Kim, Mingeol Lee, Sung Whan Choe, Min Cheol Lee, Han Gil Chung, Sang Ho Cho

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: Though acral lentiginous melanoma (ALM) is a major type of malignant melanoma, no immunohistochemical study on this type of melanoma has been reported. Objective: The purpose of this study is to analysis the immunohistochemical findings of ALM using routinely used immune markers. Methods: An immunohistochemical study was performed on paraffin sections of 20 ALMs using S-100 protein, HMB-45, MART-1, vimentin, epithelial membrane antigen (EMA) and CAM 5.2. Results: S-100 protein (95%) was found to be a more sensitive marker than either HMB-45 (80%) or MART-1 (70%) for recognizing ALM. Melanin bleaching was useful for recognizing heavily pigmented ALM using both S-100 protein and HMB-45. The intensity of HMB-45 correlated well with the melanin content. However, there was no significant correlation between the intensity of S-100 protein and the melanin content. One and two out of 20 cases stained focally with EMA and CAM5.2, respectively, but these cases stained also with HMB-45 and/or S-100 protein. Conclusions: S-100 protein and HMB-45 were relatively sensitive markers for recognizing ALM. Despite the occasional positivity for the epithelial markers in ALM, all epithelial marker-positive cases stained also with HMB-45 and/or S-100 protein. Therefore, we recommend that the panel of antibodies used for recognizing ALM should contain at least S-100 protein and HMB-45.

Original languageEnglish
Pages (from-to)123-129
Number of pages7
JournalInternational Journal of Dermatology
Volume42
Issue number2
DOIs
Publication statusPublished - 2003 Feb 1

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S100 Proteins
Melanoma
Extremities
Melanins
Mucin-1
Vimentin
Paraffin
Biomarkers
Antibodies

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Kim, You Chan ; Lee, Mingeol ; Choe, Sung Whan ; Lee, Min Cheol ; Chung, Han Gil ; Cho, Sang Ho. / Acral lentiginous melanoma : An immunohistochemical study of 20 cases. In: International Journal of Dermatology. 2003 ; Vol. 42, No. 2. pp. 123-129.
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abstract = "Background: Though acral lentiginous melanoma (ALM) is a major type of malignant melanoma, no immunohistochemical study on this type of melanoma has been reported. Objective: The purpose of this study is to analysis the immunohistochemical findings of ALM using routinely used immune markers. Methods: An immunohistochemical study was performed on paraffin sections of 20 ALMs using S-100 protein, HMB-45, MART-1, vimentin, epithelial membrane antigen (EMA) and CAM 5.2. Results: S-100 protein (95{\%}) was found to be a more sensitive marker than either HMB-45 (80{\%}) or MART-1 (70{\%}) for recognizing ALM. Melanin bleaching was useful for recognizing heavily pigmented ALM using both S-100 protein and HMB-45. The intensity of HMB-45 correlated well with the melanin content. However, there was no significant correlation between the intensity of S-100 protein and the melanin content. One and two out of 20 cases stained focally with EMA and CAM5.2, respectively, but these cases stained also with HMB-45 and/or S-100 protein. Conclusions: S-100 protein and HMB-45 were relatively sensitive markers for recognizing ALM. Despite the occasional positivity for the epithelial markers in ALM, all epithelial marker-positive cases stained also with HMB-45 and/or S-100 protein. Therefore, we recommend that the panel of antibodies used for recognizing ALM should contain at least S-100 protein and HMB-45.",
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Acral lentiginous melanoma : An immunohistochemical study of 20 cases. / Kim, You Chan; Lee, Mingeol; Choe, Sung Whan; Lee, Min Cheol; Chung, Han Gil; Cho, Sang Ho.

In: International Journal of Dermatology, Vol. 42, No. 2, 01.02.2003, p. 123-129.

Research output: Contribution to journalArticle

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T2 - An immunohistochemical study of 20 cases

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AU - Lee, Mingeol

AU - Choe, Sung Whan

AU - Lee, Min Cheol

AU - Chung, Han Gil

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N2 - Background: Though acral lentiginous melanoma (ALM) is a major type of malignant melanoma, no immunohistochemical study on this type of melanoma has been reported. Objective: The purpose of this study is to analysis the immunohistochemical findings of ALM using routinely used immune markers. Methods: An immunohistochemical study was performed on paraffin sections of 20 ALMs using S-100 protein, HMB-45, MART-1, vimentin, epithelial membrane antigen (EMA) and CAM 5.2. Results: S-100 protein (95%) was found to be a more sensitive marker than either HMB-45 (80%) or MART-1 (70%) for recognizing ALM. Melanin bleaching was useful for recognizing heavily pigmented ALM using both S-100 protein and HMB-45. The intensity of HMB-45 correlated well with the melanin content. However, there was no significant correlation between the intensity of S-100 protein and the melanin content. One and two out of 20 cases stained focally with EMA and CAM5.2, respectively, but these cases stained also with HMB-45 and/or S-100 protein. Conclusions: S-100 protein and HMB-45 were relatively sensitive markers for recognizing ALM. Despite the occasional positivity for the epithelial markers in ALM, all epithelial marker-positive cases stained also with HMB-45 and/or S-100 protein. Therefore, we recommend that the panel of antibodies used for recognizing ALM should contain at least S-100 protein and HMB-45.

AB - Background: Though acral lentiginous melanoma (ALM) is a major type of malignant melanoma, no immunohistochemical study on this type of melanoma has been reported. Objective: The purpose of this study is to analysis the immunohistochemical findings of ALM using routinely used immune markers. Methods: An immunohistochemical study was performed on paraffin sections of 20 ALMs using S-100 protein, HMB-45, MART-1, vimentin, epithelial membrane antigen (EMA) and CAM 5.2. Results: S-100 protein (95%) was found to be a more sensitive marker than either HMB-45 (80%) or MART-1 (70%) for recognizing ALM. Melanin bleaching was useful for recognizing heavily pigmented ALM using both S-100 protein and HMB-45. The intensity of HMB-45 correlated well with the melanin content. However, there was no significant correlation between the intensity of S-100 protein and the melanin content. One and two out of 20 cases stained focally with EMA and CAM5.2, respectively, but these cases stained also with HMB-45 and/or S-100 protein. Conclusions: S-100 protein and HMB-45 were relatively sensitive markers for recognizing ALM. Despite the occasional positivity for the epithelial markers in ALM, all epithelial marker-positive cases stained also with HMB-45 and/or S-100 protein. Therefore, we recommend that the panel of antibodies used for recognizing ALM should contain at least S-100 protein and HMB-45.

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