Abstract
We have previously reported that acteoside isolated from the leaves of Callicarpa dichotoma has significant neuroprotective activity against glutamate-induced neurotoxicity in primary cultured rat cortical cells. To determine the essential structural moiety within this phenylethanoid glycoside needed to exert neuroprotective activity, acteoside was hydrolyzed with acid into its aglycones, caffeic acid and 3′,4′-dihydroxylphenylethanol. Caffeic acid and 3′,4′-dihydroxylphenylethanol also showed significant neuroprotective activities. Acteoside and its aglycones inhibited glutamate-induced intracellular Ca2+ influx resulting in overproduction of nitric oxide and reduced the formation of reactive oxygen species. These compounds preserved the mitochondrial membrane potential and the activities of antioxidative enzymes, such as superoxide dismutase, glutathione reductase and glutathione peroxidase reduced by glutamate. It was followed by the preservation of the level of glutathione and finally the inhibition of membrane lipid peroxidation.
| Original language | English |
|---|---|
| Pages (from-to) | 709-716 |
| Number of pages | 8 |
| Journal | Life Sciences |
| Volume | 79 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2006 Jul 10 |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)
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Acteoside and its aglycones protect primary cultures of rat cortical cells from glutamate-induced excitotoxicity. / Koo, Kyung Ah; Kim, Seung Hyun; Oh, Tae Hwan; Kim, Young Choong.
In: Life Sciences, Vol. 79, No. 7, 10.07.2006, p. 709-716.Research output: Contribution to journal › Article
TY - JOUR
T1 - Acteoside and its aglycones protect primary cultures of rat cortical cells from glutamate-induced excitotoxicity
AU - Koo, Kyung Ah
AU - Kim, Seung Hyun
AU - Oh, Tae Hwan
AU - Kim, Young Choong
PY - 2006/7/10
Y1 - 2006/7/10
N2 - We have previously reported that acteoside isolated from the leaves of Callicarpa dichotoma has significant neuroprotective activity against glutamate-induced neurotoxicity in primary cultured rat cortical cells. To determine the essential structural moiety within this phenylethanoid glycoside needed to exert neuroprotective activity, acteoside was hydrolyzed with acid into its aglycones, caffeic acid and 3′,4′-dihydroxylphenylethanol. Caffeic acid and 3′,4′-dihydroxylphenylethanol also showed significant neuroprotective activities. Acteoside and its aglycones inhibited glutamate-induced intracellular Ca2+ influx resulting in overproduction of nitric oxide and reduced the formation of reactive oxygen species. These compounds preserved the mitochondrial membrane potential and the activities of antioxidative enzymes, such as superoxide dismutase, glutathione reductase and glutathione peroxidase reduced by glutamate. It was followed by the preservation of the level of glutathione and finally the inhibition of membrane lipid peroxidation.
AB - We have previously reported that acteoside isolated from the leaves of Callicarpa dichotoma has significant neuroprotective activity against glutamate-induced neurotoxicity in primary cultured rat cortical cells. To determine the essential structural moiety within this phenylethanoid glycoside needed to exert neuroprotective activity, acteoside was hydrolyzed with acid into its aglycones, caffeic acid and 3′,4′-dihydroxylphenylethanol. Caffeic acid and 3′,4′-dihydroxylphenylethanol also showed significant neuroprotective activities. Acteoside and its aglycones inhibited glutamate-induced intracellular Ca2+ influx resulting in overproduction of nitric oxide and reduced the formation of reactive oxygen species. These compounds preserved the mitochondrial membrane potential and the activities of antioxidative enzymes, such as superoxide dismutase, glutathione reductase and glutathione peroxidase reduced by glutamate. It was followed by the preservation of the level of glutathione and finally the inhibition of membrane lipid peroxidation.
UR - http://www.scopus.com/inward/record.url?scp=33745278030&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745278030&partnerID=8YFLogxK
U2 - 10.1016/j.lfs.2006.02.019
DO - 10.1016/j.lfs.2006.02.019
M3 - Article
C2 - 16566948
AN - SCOPUS:33745278030
VL - 79
SP - 709
EP - 716
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 7
ER -