Acteoside and its aglycones protect primary cultures of rat cortical cells from glutamate-induced excitotoxicity

Kyung Ah Koo, Seung Hyun Kim, Tae Hwan Oh, Young Choong Kim

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

We have previously reported that acteoside isolated from the leaves of Callicarpa dichotoma has significant neuroprotective activity against glutamate-induced neurotoxicity in primary cultured rat cortical cells. To determine the essential structural moiety within this phenylethanoid glycoside needed to exert neuroprotective activity, acteoside was hydrolyzed with acid into its aglycones, caffeic acid and 3′,4′-dihydroxylphenylethanol. Caffeic acid and 3′,4′-dihydroxylphenylethanol also showed significant neuroprotective activities. Acteoside and its aglycones inhibited glutamate-induced intracellular Ca2+ influx resulting in overproduction of nitric oxide and reduced the formation of reactive oxygen species. These compounds preserved the mitochondrial membrane potential and the activities of antioxidative enzymes, such as superoxide dismutase, glutathione reductase and glutathione peroxidase reduced by glutamate. It was followed by the preservation of the level of glutathione and finally the inhibition of membrane lipid peroxidation.

Original languageEnglish
Pages (from-to)709-716
Number of pages8
JournalLife Sciences
Volume79
Issue number7
DOIs
Publication statusPublished - 2006 Jul 10

Fingerprint

Rats
Glutamic Acid
Callicarpa
Glutathione Reductase
Mitochondrial Membrane Potential
Membrane Lipids
Glycosides
Glutathione Peroxidase
Lipid Peroxidation
Superoxide Dismutase
Glutathione
Reactive Oxygen Species
Nitric Oxide
Membranes
Acids
Enzymes
acteoside
caffeic acid

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

@article{7bb3edc25fb04fba98ed426e2de0a64b,
title = "Acteoside and its aglycones protect primary cultures of rat cortical cells from glutamate-induced excitotoxicity",
abstract = "We have previously reported that acteoside isolated from the leaves of Callicarpa dichotoma has significant neuroprotective activity against glutamate-induced neurotoxicity in primary cultured rat cortical cells. To determine the essential structural moiety within this phenylethanoid glycoside needed to exert neuroprotective activity, acteoside was hydrolyzed with acid into its aglycones, caffeic acid and 3′,4′-dihydroxylphenylethanol. Caffeic acid and 3′,4′-dihydroxylphenylethanol also showed significant neuroprotective activities. Acteoside and its aglycones inhibited glutamate-induced intracellular Ca2+ influx resulting in overproduction of nitric oxide and reduced the formation of reactive oxygen species. These compounds preserved the mitochondrial membrane potential and the activities of antioxidative enzymes, such as superoxide dismutase, glutathione reductase and glutathione peroxidase reduced by glutamate. It was followed by the preservation of the level of glutathione and finally the inhibition of membrane lipid peroxidation.",
author = "Koo, {Kyung Ah} and Kim, {Seung Hyun} and Oh, {Tae Hwan} and Kim, {Young Choong}",
year = "2006",
month = "7",
day = "10",
doi = "10.1016/j.lfs.2006.02.019",
language = "English",
volume = "79",
pages = "709--716",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "7",

}

Acteoside and its aglycones protect primary cultures of rat cortical cells from glutamate-induced excitotoxicity. / Koo, Kyung Ah; Kim, Seung Hyun; Oh, Tae Hwan; Kim, Young Choong.

In: Life Sciences, Vol. 79, No. 7, 10.07.2006, p. 709-716.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Acteoside and its aglycones protect primary cultures of rat cortical cells from glutamate-induced excitotoxicity

AU - Koo, Kyung Ah

AU - Kim, Seung Hyun

AU - Oh, Tae Hwan

AU - Kim, Young Choong

PY - 2006/7/10

Y1 - 2006/7/10

N2 - We have previously reported that acteoside isolated from the leaves of Callicarpa dichotoma has significant neuroprotective activity against glutamate-induced neurotoxicity in primary cultured rat cortical cells. To determine the essential structural moiety within this phenylethanoid glycoside needed to exert neuroprotective activity, acteoside was hydrolyzed with acid into its aglycones, caffeic acid and 3′,4′-dihydroxylphenylethanol. Caffeic acid and 3′,4′-dihydroxylphenylethanol also showed significant neuroprotective activities. Acteoside and its aglycones inhibited glutamate-induced intracellular Ca2+ influx resulting in overproduction of nitric oxide and reduced the formation of reactive oxygen species. These compounds preserved the mitochondrial membrane potential and the activities of antioxidative enzymes, such as superoxide dismutase, glutathione reductase and glutathione peroxidase reduced by glutamate. It was followed by the preservation of the level of glutathione and finally the inhibition of membrane lipid peroxidation.

AB - We have previously reported that acteoside isolated from the leaves of Callicarpa dichotoma has significant neuroprotective activity against glutamate-induced neurotoxicity in primary cultured rat cortical cells. To determine the essential structural moiety within this phenylethanoid glycoside needed to exert neuroprotective activity, acteoside was hydrolyzed with acid into its aglycones, caffeic acid and 3′,4′-dihydroxylphenylethanol. Caffeic acid and 3′,4′-dihydroxylphenylethanol also showed significant neuroprotective activities. Acteoside and its aglycones inhibited glutamate-induced intracellular Ca2+ influx resulting in overproduction of nitric oxide and reduced the formation of reactive oxygen species. These compounds preserved the mitochondrial membrane potential and the activities of antioxidative enzymes, such as superoxide dismutase, glutathione reductase and glutathione peroxidase reduced by glutamate. It was followed by the preservation of the level of glutathione and finally the inhibition of membrane lipid peroxidation.

UR - http://www.scopus.com/inward/record.url?scp=33745278030&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745278030&partnerID=8YFLogxK

U2 - 10.1016/j.lfs.2006.02.019

DO - 10.1016/j.lfs.2006.02.019

M3 - Article

C2 - 16566948

AN - SCOPUS:33745278030

VL - 79

SP - 709

EP - 716

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 7

ER -