TY - JOUR
T1 - Activated microvessels express vascular endothelial growth factor and integrin α(v)β3 during focal cerebral ischemia
AU - Abumiya, Takeo
AU - Lucero, Jacinta
AU - Heo, Ji Hoe
AU - Tagaya, Masafumi
AU - Koziol, James A.
AU - Copeland, Brian R.
AU - Del Zoppo, Gregory J.
PY - 1999
Y1 - 1999
N2 - Both vascular endothelial growth factor (VEGF) and integrin α(v)β3 play roles in angiogenesis. In noncerebral vascular systems, VEGF can induce endothelial integrin α(v)β3, expression. However, it is unknown whether VEGF, like integrin a(v)β3, appears in the initial response of microvessels to focal brain ischemia. Their coordinate expression in microvessels of the basal ganglia after middle cerebral artery occlusion (MCAO) in the nonhuman primate model was examined quantitatively. Cells incorporating deoxyuridine triphosphate (dUTP+) by the polymerase 1 reaction at 1 hour (n = 3), 2 hours (n = 3), and 7 days (n = 4) after MCAO defined the ischemic core (Ic) and peripheral regions. Both VEGF and integrin α(v)β3 were expressed by activated noncapillary (7.5- to 30.0-μm diameter) microvessels in the Ic region at 1 and 2 hours after MCAO. At 7 days after MCAO, the number of VEGF+, integrin α(v)β3+, or proliferating cell nuclear antigen positive microvessels had decreased within the Ic region. The expressions of VEGF, integrin α(v)β3, and proliferating cell nuclear antigen were highly correlated on the same microvessels using hierarchical log-linear statistical models. Also, VEGF and subunit α(v) messenger ribonucleic acids were coexpressed on selected microvessels. Here, noncapillary microvessels are activated specifically early during a focal cerebral ischemic insult and rapidly express VEGF and integrin α(v)β3 together.
AB - Both vascular endothelial growth factor (VEGF) and integrin α(v)β3 play roles in angiogenesis. In noncerebral vascular systems, VEGF can induce endothelial integrin α(v)β3, expression. However, it is unknown whether VEGF, like integrin a(v)β3, appears in the initial response of microvessels to focal brain ischemia. Their coordinate expression in microvessels of the basal ganglia after middle cerebral artery occlusion (MCAO) in the nonhuman primate model was examined quantitatively. Cells incorporating deoxyuridine triphosphate (dUTP+) by the polymerase 1 reaction at 1 hour (n = 3), 2 hours (n = 3), and 7 days (n = 4) after MCAO defined the ischemic core (Ic) and peripheral regions. Both VEGF and integrin α(v)β3 were expressed by activated noncapillary (7.5- to 30.0-μm diameter) microvessels in the Ic region at 1 and 2 hours after MCAO. At 7 days after MCAO, the number of VEGF+, integrin α(v)β3+, or proliferating cell nuclear antigen positive microvessels had decreased within the Ic region. The expressions of VEGF, integrin α(v)β3, and proliferating cell nuclear antigen were highly correlated on the same microvessels using hierarchical log-linear statistical models. Also, VEGF and subunit α(v) messenger ribonucleic acids were coexpressed on selected microvessels. Here, noncapillary microvessels are activated specifically early during a focal cerebral ischemic insult and rapidly express VEGF and integrin α(v)β3 together.
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U2 - 10.1097/00004647-199909000-00012
DO - 10.1097/00004647-199909000-00012
M3 - Article
C2 - 10478656
AN - SCOPUS:0033496252
VL - 19
SP - 1038
EP - 1050
JO - Journal of Cerebral Blood Flow and Metabolism
JF - Journal of Cerebral Blood Flow and Metabolism
SN - 0271-678X
IS - 9
ER -