Activated microvessels express vascular endothelial growth factor and integrin α(v)β3 during focal cerebral ischemia

Takeo Abumiya, Jacinta Lucero, Jihoe Heo, Masafumi Tagaya, James A. Koziol, Brian R. Copeland, Gregory J. Del Zoppo

Research output: Contribution to journalArticle

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Abstract

Both vascular endothelial growth factor (VEGF) and integrin α(v)β3 play roles in angiogenesis. In noncerebral vascular systems, VEGF can induce endothelial integrin α(v)β3, expression. However, it is unknown whether VEGF, like integrin a(v)β3, appears in the initial response of microvessels to focal brain ischemia. Their coordinate expression in microvessels of the basal ganglia after middle cerebral artery occlusion (MCAO) in the nonhuman primate model was examined quantitatively. Cells incorporating deoxyuridine triphosphate (dUTP+) by the polymerase 1 reaction at 1 hour (n = 3), 2 hours (n = 3), and 7 days (n = 4) after MCAO defined the ischemic core (Ic) and peripheral regions. Both VEGF and integrin α(v)β3 were expressed by activated noncapillary (7.5- to 30.0-μm diameter) microvessels in the Ic region at 1 and 2 hours after MCAO. At 7 days after MCAO, the number of VEGF+, integrin α(v)β3 +, or proliferating cell nuclear antigen positive microvessels had decreased within the Ic region. The expressions of VEGF, integrin α(v)β3, and proliferating cell nuclear antigen were highly correlated on the same microvessels using hierarchical log-linear statistical models. Also, VEGF and subunit α(v) messenger ribonucleic acids were coexpressed on selected microvessels. Here, noncapillary microvessels are activated specifically early during a focal cerebral ischemic insult and rapidly express VEGF and integrin α(v)β3 together.

Original languageEnglish
Pages (from-to)1038-1050
Number of pages13
JournalJournal of Cerebral Blood Flow and Metabolism
Volume19
Issue number9
DOIs
Publication statusPublished - 1999 Jan 1

Fingerprint

Microvessels
Brain Ischemia
Integrins
Vascular Endothelial Growth Factor A
Middle Cerebral Artery Infarction
Proliferating Cell Nuclear Antigen
Statistical Models
Basal Ganglia
Primates
Blood Vessels
Linear Models
RNA

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Abumiya, Takeo ; Lucero, Jacinta ; Heo, Jihoe ; Tagaya, Masafumi ; Koziol, James A. ; Copeland, Brian R. ; Del Zoppo, Gregory J. / Activated microvessels express vascular endothelial growth factor and integrin α(v)β3 during focal cerebral ischemia. In: Journal of Cerebral Blood Flow and Metabolism. 1999 ; Vol. 19, No. 9. pp. 1038-1050.
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Activated microvessels express vascular endothelial growth factor and integrin α(v)β3 during focal cerebral ischemia. / Abumiya, Takeo; Lucero, Jacinta; Heo, Jihoe; Tagaya, Masafumi; Koziol, James A.; Copeland, Brian R.; Del Zoppo, Gregory J.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 19, No. 9, 01.01.1999, p. 1038-1050.

Research output: Contribution to journalArticle

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AU - Abumiya, Takeo

AU - Lucero, Jacinta

AU - Heo, Jihoe

AU - Tagaya, Masafumi

AU - Koziol, James A.

AU - Copeland, Brian R.

AU - Del Zoppo, Gregory J.

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