Activation of G proteins by aluminum fluoride enhances RANKLmediated osteoclastogenesis

Boryung Park, Yu Mi Yang, Byung Jai Choi, Min Seuk Kim, Dong Min Shin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Receptor activator of NF-κB ligand (RANKL)-induced osteoclastogenesis is accompanied by intracellular Ca2+ mobilization in a form of oscillations, which plays essential roles by activating sequentially Ca 2+ /calmodulin-dependent protein kinase, calcineurin and NFATc1, necessary in the osteoclast differentiation. However, it is not known whether Ca2+ mobilization which is evoked in RANKL-independent way induces to differentiate into osteoclasts. In present study, we investigated Ca 2+ mobilization induced by aluminum fluoride (AlF4 .), a G-protein activator, with or without RANKL and the effects of AlF4 . on the osteoclastogenesis in primary cultured mouse bone marrow-derived macrophages (BMMs). We show here that AlF4 . induces intracellular Ca2+ concentration ([Ca2+]i) oscillations, which is dependent on extracellular Ca2+ influx. Notably, co-stimulation of AlF4 . with RANKL resulted in enhanced NFATc1 expression and formation of tartrate-resistant acid phosphatase (TRAP) positive multinucleated cells. Additionally, we confirmed that mitogen-activated protein kinase (MAPK) is also activated by AlF4 .. Taken together, these results demonstrate that G-protein would be a novel modulator responsible for [Ca2+]i oscillations and MAPK activation which lead to enhancement of RANKL-mediated osteoclastogenesis.

Original languageEnglish
Pages (from-to)427-433
Number of pages7
JournalKorean Journal of Physiology and Pharmacology
Volume17
Issue number5
DOIs
Publication statusPublished - 2013 Oct 1

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GTP-Binding Proteins
Osteogenesis
Osteoclasts
Mitogen-Activated Protein Kinases
Calcium-Calmodulin-Dependent Protein Kinases
Calcineurin
Macrophages
aluminum fluoride
tetrafluoroaluminate
Ligands

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pharmacology

Cite this

Park, Boryung ; Yang, Yu Mi ; Choi, Byung Jai ; Kim, Min Seuk ; Shin, Dong Min. / Activation of G proteins by aluminum fluoride enhances RANKLmediated osteoclastogenesis. In: Korean Journal of Physiology and Pharmacology. 2013 ; Vol. 17, No. 5. pp. 427-433.
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Activation of G proteins by aluminum fluoride enhances RANKLmediated osteoclastogenesis. / Park, Boryung; Yang, Yu Mi; Choi, Byung Jai; Kim, Min Seuk; Shin, Dong Min.

In: Korean Journal of Physiology and Pharmacology, Vol. 17, No. 5, 01.10.2013, p. 427-433.

Research output: Contribution to journalArticle

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