Activation of MEK-ERK by heregulin-β1 promotes the development of cardiomyocytes derived from ES cells

Hoe Suk Kim, Jin Won Cho, Kyoko Hidaka, Takayuki Morisaki

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33 Citations (Scopus)

Abstract

We have previously shown that heregulin-beta1 (HRG-β1) was involved in the development and survival of cardiomyocytes derived from embryonic stem (ES) cells. This study was conducted to investigate the intracellular signal mechanisms by which HRG-β1 stimulates cardiogenesis in ES cells. The treatment with ErbB receptor inhibitor decreased the population of cardiomyocytes and transcripts levels of cardiac genes (Nkx2.5, β-MHC, cTnI, and MLC2a). The phosphorylation of ERK and development of cardiomyocytes by treatment with HRG-β1 was suppressed upon treatment with MEK1 inhibitor. Furthermore, cardiomyocytes and level of MHC protein were significantly increased by overexpression of wild type MEK1 or constitutive active MEK1, but not dominant negative MEK1. These results suggest that HRG-β1 promotes the development of cardiomyocytes predominantly by activation of MEK-ERK.

Original languageEnglish
Pages (from-to)732-738
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume361
Issue number3
DOIs
Publication statusPublished - 2007 Sep 28

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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