Activator protein-1 mediates docosahexaenoic acid-induced apoptosis of human gastric cancer cells

Sun En Lee, Joo Weon Lim, Hye Young Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

27 Citations (Scopus)

Abstract

Docosahexaenoic acid (DHA) shows anti-inflammatory and/or anticancer effects in some cells. Activator protein-1 (AP-1) regulates cellular proliferation and apoptosis. Although recent studies demonstrate the association between gastric cancer risk and DHA, the exact molecular mechanism has not been clarified. We investigated whether AP-1 mediates DHA-induced apoptosis of gastric cancer cells. We found that DHA induced cell death and DNA fragmentation in parallel with the activation of extracellular signal-regulated kinases (ERK) and c-Jun N-terminal kinases (JNK) as well as AP-1. DHA increased the protein levels of p53, cytochrome c, and Bax in gastric cancer cells. DHA-induced DNA fragmentation and protein levels of p53, cytochrome c, and Bax were inhibited in the cells transfected with c-jun dominant-negative mutant (TAM67). Because JNK and ERK are upstream signaling for AP-1 activation, we suggest that DHA-induced activation of AP-1 may mediate apoptosis of gastric cancer cells by inducing the expression of apoptotic genes in gastric cancer cells.

Original languageEnglish
Title of host publicationNatural Compounds and Their Role in Apoptotic Cell Signaling Pathways
PublisherBlackwell Publishing Inc.
Pages163-169
Number of pages7
ISBN (Print)9781573317375
DOIs
Publication statusPublished - 2009 Jan 1

Publication series

NameAnnals of the New York Academy of Sciences
Volume1171
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Fingerprint

Docosahexaenoic Acids
Transcription Factor AP-1
Stomach Neoplasms
Cells
Apoptosis
Chemical activation
Extracellular Signal-Regulated MAP Kinases
DNA Fragmentation
Cytochromes c
JNK Mitogen-Activated Protein Kinases
DNA
Cell death
Protein
Cancer
Proteins
Cell Death
Anti-Inflammatory Agents
Phosphotransferases
Genes
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Lee, S. E., Lim, J. W., & Kim, H. Y. (2009). Activator protein-1 mediates docosahexaenoic acid-induced apoptosis of human gastric cancer cells. In Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways (pp. 163-169). (Annals of the New York Academy of Sciences; Vol. 1171). Blackwell Publishing Inc.. https://doi.org/10.1111/j.1749-6632.2009.04716.x
Lee, Sun En ; Lim, Joo Weon ; Kim, Hye Young. / Activator protein-1 mediates docosahexaenoic acid-induced apoptosis of human gastric cancer cells. Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Blackwell Publishing Inc., 2009. pp. 163-169 (Annals of the New York Academy of Sciences).
@inproceedings{bf1b4699ec8c43b9bfa4922c9d27c354,
title = "Activator protein-1 mediates docosahexaenoic acid-induced apoptosis of human gastric cancer cells",
abstract = "Docosahexaenoic acid (DHA) shows anti-inflammatory and/or anticancer effects in some cells. Activator protein-1 (AP-1) regulates cellular proliferation and apoptosis. Although recent studies demonstrate the association between gastric cancer risk and DHA, the exact molecular mechanism has not been clarified. We investigated whether AP-1 mediates DHA-induced apoptosis of gastric cancer cells. We found that DHA induced cell death and DNA fragmentation in parallel with the activation of extracellular signal-regulated kinases (ERK) and c-Jun N-terminal kinases (JNK) as well as AP-1. DHA increased the protein levels of p53, cytochrome c, and Bax in gastric cancer cells. DHA-induced DNA fragmentation and protein levels of p53, cytochrome c, and Bax were inhibited in the cells transfected with c-jun dominant-negative mutant (TAM67). Because JNK and ERK are upstream signaling for AP-1 activation, we suggest that DHA-induced activation of AP-1 may mediate apoptosis of gastric cancer cells by inducing the expression of apoptotic genes in gastric cancer cells.",
author = "Lee, {Sun En} and Lim, {Joo Weon} and Kim, {Hye Young}",
year = "2009",
month = "1",
day = "1",
doi = "10.1111/j.1749-6632.2009.04716.x",
language = "English",
isbn = "9781573317375",
series = "Annals of the New York Academy of Sciences",
publisher = "Blackwell Publishing Inc.",
pages = "163--169",
booktitle = "Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways",

}

Lee, SE, Lim, JW & Kim, HY 2009, Activator protein-1 mediates docosahexaenoic acid-induced apoptosis of human gastric cancer cells. in Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Annals of the New York Academy of Sciences, vol. 1171, Blackwell Publishing Inc., pp. 163-169. https://doi.org/10.1111/j.1749-6632.2009.04716.x

Activator protein-1 mediates docosahexaenoic acid-induced apoptosis of human gastric cancer cells. / Lee, Sun En; Lim, Joo Weon; Kim, Hye Young.

Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Blackwell Publishing Inc., 2009. p. 163-169 (Annals of the New York Academy of Sciences; Vol. 1171).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Activator protein-1 mediates docosahexaenoic acid-induced apoptosis of human gastric cancer cells

AU - Lee, Sun En

AU - Lim, Joo Weon

AU - Kim, Hye Young

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Docosahexaenoic acid (DHA) shows anti-inflammatory and/or anticancer effects in some cells. Activator protein-1 (AP-1) regulates cellular proliferation and apoptosis. Although recent studies demonstrate the association between gastric cancer risk and DHA, the exact molecular mechanism has not been clarified. We investigated whether AP-1 mediates DHA-induced apoptosis of gastric cancer cells. We found that DHA induced cell death and DNA fragmentation in parallel with the activation of extracellular signal-regulated kinases (ERK) and c-Jun N-terminal kinases (JNK) as well as AP-1. DHA increased the protein levels of p53, cytochrome c, and Bax in gastric cancer cells. DHA-induced DNA fragmentation and protein levels of p53, cytochrome c, and Bax were inhibited in the cells transfected with c-jun dominant-negative mutant (TAM67). Because JNK and ERK are upstream signaling for AP-1 activation, we suggest that DHA-induced activation of AP-1 may mediate apoptosis of gastric cancer cells by inducing the expression of apoptotic genes in gastric cancer cells.

AB - Docosahexaenoic acid (DHA) shows anti-inflammatory and/or anticancer effects in some cells. Activator protein-1 (AP-1) regulates cellular proliferation and apoptosis. Although recent studies demonstrate the association between gastric cancer risk and DHA, the exact molecular mechanism has not been clarified. We investigated whether AP-1 mediates DHA-induced apoptosis of gastric cancer cells. We found that DHA induced cell death and DNA fragmentation in parallel with the activation of extracellular signal-regulated kinases (ERK) and c-Jun N-terminal kinases (JNK) as well as AP-1. DHA increased the protein levels of p53, cytochrome c, and Bax in gastric cancer cells. DHA-induced DNA fragmentation and protein levels of p53, cytochrome c, and Bax were inhibited in the cells transfected with c-jun dominant-negative mutant (TAM67). Because JNK and ERK are upstream signaling for AP-1 activation, we suggest that DHA-induced activation of AP-1 may mediate apoptosis of gastric cancer cells by inducing the expression of apoptotic genes in gastric cancer cells.

UR - http://www.scopus.com/inward/record.url?scp=69149090288&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69149090288&partnerID=8YFLogxK

U2 - 10.1111/j.1749-6632.2009.04716.x

DO - 10.1111/j.1749-6632.2009.04716.x

M3 - Conference contribution

SN - 9781573317375

T3 - Annals of the New York Academy of Sciences

SP - 163

EP - 169

BT - Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways

PB - Blackwell Publishing Inc.

ER -

Lee SE, Lim JW, Kim HY. Activator protein-1 mediates docosahexaenoic acid-induced apoptosis of human gastric cancer cells. In Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Blackwell Publishing Inc. 2009. p. 163-169. (Annals of the New York Academy of Sciences). https://doi.org/10.1111/j.1749-6632.2009.04716.x