Adipose-derived stem cell-containing hyaluronic acid/alginate hydrogel improves vocal fold wound healing

Young Mo Kim, Se H. Oh, Jeong Seok Choi, Songyi Lee, Jeong C. Ra, Jin H. Lee, JaeYoul Lim

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Objectives/Hypothesis The purpose of this study was to investigate the regenerative efficacy of an injectable hyaluronic acid/mildly cross-linked alginate hydrogel (HA/ALG hydrogel) containing human adipose-derived mesenchymal stem cells (hAdMSCs) for vocal fold (VF) wound healing. Study Design Animal research. Methods HA/ALG hydrogel containing hAdMSCs was injected into the VFs of rabbits immediately after direct injury. Endoscopic evaluations were performed at 1 and 3 months after injury, and functional evaluations of mucosal vibration and viscoelastic properties were carried out posteuthanization at 3 months after injury. Histopathologic and immunohistochemical evaluations of extracellular matrix (ECM) components and of hepatocyte growth factor (HGF) activity were conducted in injured VFs. The engraftment of implanted hAdMSCs was investigated by detecting fluorescent-labeled cells. Results The administration of hAdMSCs and hAdMSCs in HA/ALG hydrogel produced better macroscopic morphologies than the injection of phosphate-buffered saline (PBS). Histologic evaluations revealed that treatment with hAdMSCs in HA/ALG produced more favorable ECM changes than hAdMSC. In particular, the treatment of hAdMSCs in HA/ALG hydrogel ameliorated excessive deposition of collagen type I and increased HGF activity in regenerating VFs. hAdMSCs in HA/ALG-treated VFs also exhibited functional improvements in viscoelastic properties. hAdMSCs in HA/ALG remained viable in recipient VFs at 1 month after transplantation, and some were observed to be fostered to differentiate into fibroblasts. Conclusions The findings of the present study suggest that HA/ALG hydrogel is a promising biomaterial for prolonging the retention time of stem cells in VFs and for promotion of VF wound healing.

Original languageEnglish
Pages (from-to)64-72
Number of pages9
JournalLaryngoscope
Volume124
Issue number3
DOIs
Publication statusPublished - 2014 Mar 1

Fingerprint

Vocal Cords
Hydrogel
Hyaluronic Acid
Mesenchymal Stromal Cells
Wound Healing
Stem Cells
Hepatocyte Growth Factor
Extracellular Matrix
Wounds and Injuries
alginic acid
Injections
Biocompatible Materials
Collagen Type I
Vibration
Fibroblasts
Transplantation
Phosphates
Rabbits

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Kim, Young Mo ; Oh, Se H. ; Choi, Jeong Seok ; Lee, Songyi ; Ra, Jeong C. ; Lee, Jin H. ; Lim, JaeYoul. / Adipose-derived stem cell-containing hyaluronic acid/alginate hydrogel improves vocal fold wound healing. In: Laryngoscope. 2014 ; Vol. 124, No. 3. pp. 64-72.
@article{6ab8097656794fc889ad00875d34adf6,
title = "Adipose-derived stem cell-containing hyaluronic acid/alginate hydrogel improves vocal fold wound healing",
abstract = "Objectives/Hypothesis The purpose of this study was to investigate the regenerative efficacy of an injectable hyaluronic acid/mildly cross-linked alginate hydrogel (HA/ALG hydrogel) containing human adipose-derived mesenchymal stem cells (hAdMSCs) for vocal fold (VF) wound healing. Study Design Animal research. Methods HA/ALG hydrogel containing hAdMSCs was injected into the VFs of rabbits immediately after direct injury. Endoscopic evaluations were performed at 1 and 3 months after injury, and functional evaluations of mucosal vibration and viscoelastic properties were carried out posteuthanization at 3 months after injury. Histopathologic and immunohistochemical evaluations of extracellular matrix (ECM) components and of hepatocyte growth factor (HGF) activity were conducted in injured VFs. The engraftment of implanted hAdMSCs was investigated by detecting fluorescent-labeled cells. Results The administration of hAdMSCs and hAdMSCs in HA/ALG hydrogel produced better macroscopic morphologies than the injection of phosphate-buffered saline (PBS). Histologic evaluations revealed that treatment with hAdMSCs in HA/ALG produced more favorable ECM changes than hAdMSC. In particular, the treatment of hAdMSCs in HA/ALG hydrogel ameliorated excessive deposition of collagen type I and increased HGF activity in regenerating VFs. hAdMSCs in HA/ALG-treated VFs also exhibited functional improvements in viscoelastic properties. hAdMSCs in HA/ALG remained viable in recipient VFs at 1 month after transplantation, and some were observed to be fostered to differentiate into fibroblasts. Conclusions The findings of the present study suggest that HA/ALG hydrogel is a promising biomaterial for prolonging the retention time of stem cells in VFs and for promotion of VF wound healing.",
author = "Kim, {Young Mo} and Oh, {Se H.} and Choi, {Jeong Seok} and Songyi Lee and Ra, {Jeong C.} and Lee, {Jin H.} and JaeYoul Lim",
year = "2014",
month = "3",
day = "1",
doi = "10.1002/lary.24405",
language = "English",
volume = "124",
pages = "64--72",
journal = "Laryngoscope",
issn = "0023-852X",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

Adipose-derived stem cell-containing hyaluronic acid/alginate hydrogel improves vocal fold wound healing. / Kim, Young Mo; Oh, Se H.; Choi, Jeong Seok; Lee, Songyi; Ra, Jeong C.; Lee, Jin H.; Lim, JaeYoul.

In: Laryngoscope, Vol. 124, No. 3, 01.03.2014, p. 64-72.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Adipose-derived stem cell-containing hyaluronic acid/alginate hydrogel improves vocal fold wound healing

AU - Kim, Young Mo

AU - Oh, Se H.

AU - Choi, Jeong Seok

AU - Lee, Songyi

AU - Ra, Jeong C.

AU - Lee, Jin H.

AU - Lim, JaeYoul

PY - 2014/3/1

Y1 - 2014/3/1

N2 - Objectives/Hypothesis The purpose of this study was to investigate the regenerative efficacy of an injectable hyaluronic acid/mildly cross-linked alginate hydrogel (HA/ALG hydrogel) containing human adipose-derived mesenchymal stem cells (hAdMSCs) for vocal fold (VF) wound healing. Study Design Animal research. Methods HA/ALG hydrogel containing hAdMSCs was injected into the VFs of rabbits immediately after direct injury. Endoscopic evaluations were performed at 1 and 3 months after injury, and functional evaluations of mucosal vibration and viscoelastic properties were carried out posteuthanization at 3 months after injury. Histopathologic and immunohistochemical evaluations of extracellular matrix (ECM) components and of hepatocyte growth factor (HGF) activity were conducted in injured VFs. The engraftment of implanted hAdMSCs was investigated by detecting fluorescent-labeled cells. Results The administration of hAdMSCs and hAdMSCs in HA/ALG hydrogel produced better macroscopic morphologies than the injection of phosphate-buffered saline (PBS). Histologic evaluations revealed that treatment with hAdMSCs in HA/ALG produced more favorable ECM changes than hAdMSC. In particular, the treatment of hAdMSCs in HA/ALG hydrogel ameliorated excessive deposition of collagen type I and increased HGF activity in regenerating VFs. hAdMSCs in HA/ALG-treated VFs also exhibited functional improvements in viscoelastic properties. hAdMSCs in HA/ALG remained viable in recipient VFs at 1 month after transplantation, and some were observed to be fostered to differentiate into fibroblasts. Conclusions The findings of the present study suggest that HA/ALG hydrogel is a promising biomaterial for prolonging the retention time of stem cells in VFs and for promotion of VF wound healing.

AB - Objectives/Hypothesis The purpose of this study was to investigate the regenerative efficacy of an injectable hyaluronic acid/mildly cross-linked alginate hydrogel (HA/ALG hydrogel) containing human adipose-derived mesenchymal stem cells (hAdMSCs) for vocal fold (VF) wound healing. Study Design Animal research. Methods HA/ALG hydrogel containing hAdMSCs was injected into the VFs of rabbits immediately after direct injury. Endoscopic evaluations were performed at 1 and 3 months after injury, and functional evaluations of mucosal vibration and viscoelastic properties were carried out posteuthanization at 3 months after injury. Histopathologic and immunohistochemical evaluations of extracellular matrix (ECM) components and of hepatocyte growth factor (HGF) activity were conducted in injured VFs. The engraftment of implanted hAdMSCs was investigated by detecting fluorescent-labeled cells. Results The administration of hAdMSCs and hAdMSCs in HA/ALG hydrogel produced better macroscopic morphologies than the injection of phosphate-buffered saline (PBS). Histologic evaluations revealed that treatment with hAdMSCs in HA/ALG produced more favorable ECM changes than hAdMSC. In particular, the treatment of hAdMSCs in HA/ALG hydrogel ameliorated excessive deposition of collagen type I and increased HGF activity in regenerating VFs. hAdMSCs in HA/ALG-treated VFs also exhibited functional improvements in viscoelastic properties. hAdMSCs in HA/ALG remained viable in recipient VFs at 1 month after transplantation, and some were observed to be fostered to differentiate into fibroblasts. Conclusions The findings of the present study suggest that HA/ALG hydrogel is a promising biomaterial for prolonging the retention time of stem cells in VFs and for promotion of VF wound healing.

UR - http://www.scopus.com/inward/record.url?scp=84894483220&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84894483220&partnerID=8YFLogxK

U2 - 10.1002/lary.24405

DO - 10.1002/lary.24405

M3 - Article

VL - 124

SP - 64

EP - 72

JO - Laryngoscope

JF - Laryngoscope

SN - 0023-852X

IS - 3

ER -