Adipose tissue-derived mesenchymal stem cells cultured at high density express IFN-β and suppress the growth of MCF-7 human breast cancer cells

Hoon Ryu, Ji Eun Oh, Ki Jong Rhee, Soon Koo Baik, Jiye Kim, Seong Joon Kang, Joon Hyung Sohn, Eunhee Choi, Ha Cheol Shin, Yong Man Kim, Hyun Soo Kim, Keum Seok Bae, Young Woo Eom

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Although it has been reported that mesenchymal stem cells (MSCs) suppress tumor growth in vitro and in vivo, little is known about the underlying molecular mechanisms. We found that type I interferon is expressed in adipose tissue-derived stem cells (ASCs) cultured at high density, and ASCs and their conditioned medium (ASC-CM) suppress the growth of MCF-7 cells in vitro. Growth inhibition was amplified by glucose deprivation that resulted from high density culture of ASCs after 3. days. The cytotoxic effect of the ASC-CM obtained from high density culture of ASCs was neutralized by anti-IFN-β antibody. STAT1 was phosphorylated in MCF-7 cells treated with ASC-CM, and JAK1/JAK2 inhibitor treatment decreased STAT1 phosphorylation. The cytotoxic effect of ASC-CM was reduced especially by JAK1 inhibitors in MCF-7 cells. Our findings suggest that ASCs cultured at high density express type I interferons, which suppresses tumor growth via STAT1 activation resulting from IFN-β secretion in MCF-7 breast cancer cells.

Original languageEnglish
Pages (from-to)220-227
Number of pages8
JournalCancer Letters
Volume352
Issue number2
DOIs
Publication statusPublished - 2014 Oct 1

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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