Adipose tissue-derived mesenchymal stem cells cultured at high density express IFN-β and TRAIL and suppress the growth of H460 human lung cancer cells

Pil Young Jung, Hoon Ryu, Kijong Rhee, Soonjae Hwang, Chang Gun Lee, Sun Yeong Gwon, Jiye Kim, Juwon Kim, Byungsu Yoo, Soonkoo Baik, Keumseok Bae, Young Woo Eom

Research output: Contribution to journalArticle

Abstract

Although mesenchymal stem cells (MSCs) have been reported to inhibit tumor growth, the mechanism controlling this tumor suppression function is unclear. Here, we report that high-density (40,000 cells/cm2) cultured adipose tissue-derived MSCs (40K-ASCs) expressed interferon (IFN)-β and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL); we also found that serum deprivation during cell culture induced the expression of IFN-β and TRAIL. In addition, the mRNA expression of IFN-β but not TRAIL, was increased during the washing step required for the transplantation of normal-density (5000 cells/cm2) cultured ASCs (5K-ASCs). When the human lung cancer cell line H460 was co-cultured with 40K-ASCs, necrotic cell death was dramatically increased in vitro. When ASCs were injected after four washes, both 5K-ASCs and 40K-ASCs substantially reduced tumor weight in H460-derived cancer animal models. These results suggest that serum deprivation during the culture of 40K-ASCs or during the washing step of 5K-ASCs can induce IFN-β and/or TRAIL expression, ultimately leading to the tumor suppression capability of ASCs.

LanguageEnglish
Pages202-210
Number of pages9
JournalCancer Letters
Volume440-441
DOIs
Publication statusPublished - 2019 Jan 1

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Interferon-beta
Mesenchymal Stromal Cells
Adipose Tissue
Lung Neoplasms
Growth
Cultured Cells
Neoplasms
Tumor Burden
Serum
Cell Death
Animal Models
Cell Culture Techniques
Tumor Necrosis Factor-alpha
Cell Count
Transplantation
Apoptosis
Ligands
Cell Line
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Jung, Pil Young ; Ryu, Hoon ; Rhee, Kijong ; Hwang, Soonjae ; Lee, Chang Gun ; Gwon, Sun Yeong ; Kim, Jiye ; Kim, Juwon ; Yoo, Byungsu ; Baik, Soonkoo ; Bae, Keumseok ; Eom, Young Woo. / Adipose tissue-derived mesenchymal stem cells cultured at high density express IFN-β and TRAIL and suppress the growth of H460 human lung cancer cells. In: Cancer Letters. 2019 ; Vol. 440-441. pp. 202-210.
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Adipose tissue-derived mesenchymal stem cells cultured at high density express IFN-β and TRAIL and suppress the growth of H460 human lung cancer cells. / Jung, Pil Young; Ryu, Hoon; Rhee, Kijong; Hwang, Soonjae; Lee, Chang Gun; Gwon, Sun Yeong; Kim, Jiye; Kim, Juwon; Yoo, Byungsu; Baik, Soonkoo; Bae, Keumseok; Eom, Young Woo.

In: Cancer Letters, Vol. 440-441, 01.01.2019, p. 202-210.

Research output: Contribution to journalArticle

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AU - Jung, Pil Young

AU - Ryu, Hoon

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AU - Hwang, Soonjae

AU - Lee, Chang Gun

AU - Gwon, Sun Yeong

AU - Kim, Jiye

AU - Kim, Juwon

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AU - Baik, Soonkoo

AU - Bae, Keumseok

AU - Eom, Young Woo

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