Adipose tissue-derived mesenchymal stem cells cultured at high density express IFN-β and TRAIL and suppress the growth of H460 human lung cancer cells

Pil Young Jung, Hoon Ryu, Ki Jong Rhee, Soonjae Hwang, Chang Gun Lee, Sun Yeong Gwon, Jiye Kim, Juwon Kim, Byung Su Yoo, Soon Koo Baik, Keum Seok Bae, Young Woo Eom

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Although mesenchymal stem cells (MSCs) have been reported to inhibit tumor growth, the mechanism controlling this tumor suppression function is unclear. Here, we report that high-density (40,000 cells/cm2) cultured adipose tissue-derived MSCs (40K-ASCs) expressed interferon (IFN)-β and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL); we also found that serum deprivation during cell culture induced the expression of IFN-β and TRAIL. In addition, the mRNA expression of IFN-β but not TRAIL, was increased during the washing step required for the transplantation of normal-density (5000 cells/cm2) cultured ASCs (5K-ASCs). When the human lung cancer cell line H460 was co-cultured with 40K-ASCs, necrotic cell death was dramatically increased in vitro. When ASCs were injected after four washes, both 5K-ASCs and 40K-ASCs substantially reduced tumor weight in H460-derived cancer animal models. These results suggest that serum deprivation during the culture of 40K-ASCs or during the washing step of 5K-ASCs can induce IFN-β and/or TRAIL expression, ultimately leading to the tumor suppression capability of ASCs.

Original languageEnglish
Pages (from-to)202-210
Number of pages9
JournalCancer Letters
Volume440-441
DOIs
Publication statusPublished - 2019 Jan

Bibliographical note

Funding Information:
The authors thank Editage ( www.editage.co.kr ) for English language editing. This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute , funded by the Ministry of Health & Welfare, Republic of Korea (grant number HI17C1365 ); the Basic Science Research program (grant number NRF-2017R1C1B5017842 ); and the Small Grant for Exploratory Research (SGER) Program (grant numbers NRF-2017R1D1A1A02018088 , -2017R1D1A1A02019212 ) through the National Research Foundation of Korea, funded by the Korean government (Ministry of Education) .

Publisher Copyright:
© 2018 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Adipose tissue-derived mesenchymal stem cells cultured at high density express IFN-β and TRAIL and suppress the growth of H460 human lung cancer cells'. Together they form a unique fingerprint.

Cite this