In order to elucidate the relations between the amount of surface functional groups and interaction forces, BSA adsorption experiments were performed, using highly carboxylated PS/PMAA microspheres as well as conventional ones. Two kinds of interaction forces were considered in this study, hydrogen bonding and hydrophobic interactions, while ionic interactions were assumed to be small or constant. Hydrophobic interactions were dominant in the low Nc (number density of surface carboxyl groups) region, below 1 carboxyl group nm-2, and were relatively sensitive to pH, while hydrogen bonding was dominant in the high Nc region, above 2 carboxyl groups nm-2, irrespective of pH. The transition region between these two interaction forces was 1 ∼ 2 carboxyl groups nm-2 on the surface of a microsphere. When comparing the two extremes of hydrophobic interaction and hydrogen bonding, the latter was stronger, which was different from earlier studies. It can be explained that the conventional carboxylated microspheres do not have enough carboxyl groups on their surfaces. Adsorption constant K diminished as Nc increased, which means that the affinity itself is reduced by hydrogen bonding. Adsorbed layer thicknesses calculated from the Cm (adsorbed amount in equilibrium) were 4.66 ∼ 7.08 nm; this means that the BSA molecules exist between the side-on and end-on mode.
Bibliographical noteFunding Information:
Funding for this research was provided by the Korea Science and Engineering Foundation (KOSEF), Project Number 91-03-00-04.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Surfaces, Coatings and Films
- Colloid and Surface Chemistry