Advances and challenges in neurochemical profiling of biological samples using mass spectrometry coupled with separation methods

Wonwoong Lee, Na Hyun Park, Yongchan Lee, Ki Hyun Kim, Jongki Hong

Research output: Contribution to journalReview article

Abstract

Neurochemicals are mainly distributed in the central nervous system, and related to various neurological functions and disorders. Because neurochemicals are biosynthesized and metabolized through multiple pathways, it is difficult to understand the overall nervous system or to diagnose neurological disorders based on certain neurochemical levels. Therefore, to learn more about the nervous system and neurological disorders, metabolomics approaches should be used to profile the metabolic pathways of neurochemicals. To date, the neurochemical profiling has been pursued by various methods, especially including mass spectrometry (MS) coupled with conventional chromatographic techniques, which can sensitively and selectively determine numerous neurochemicals in biological samples. Nevertheless, the development of chromatographic MS-based analytical platforms to profile neurochemicals in biological samples has faced several challenges. This review was organized to help characterize neurochemicals using descriptions of the basic procedures (sample preparation and chromatographic MS detection). Moreover, we describe clinical applications and performance evaluation of these techniques.

Original languageEnglish
Pages (from-to)159-168
Number of pages10
JournalTrAC - Trends in Analytical Chemistry
Volume106
DOIs
Publication statusPublished - 2018 Sep 1

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Neurology
Mass spectrometry

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy

Cite this

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title = "Advances and challenges in neurochemical profiling of biological samples using mass spectrometry coupled with separation methods",
abstract = "Neurochemicals are mainly distributed in the central nervous system, and related to various neurological functions and disorders. Because neurochemicals are biosynthesized and metabolized through multiple pathways, it is difficult to understand the overall nervous system or to diagnose neurological disorders based on certain neurochemical levels. Therefore, to learn more about the nervous system and neurological disorders, metabolomics approaches should be used to profile the metabolic pathways of neurochemicals. To date, the neurochemical profiling has been pursued by various methods, especially including mass spectrometry (MS) coupled with conventional chromatographic techniques, which can sensitively and selectively determine numerous neurochemicals in biological samples. Nevertheless, the development of chromatographic MS-based analytical platforms to profile neurochemicals in biological samples has faced several challenges. This review was organized to help characterize neurochemicals using descriptions of the basic procedures (sample preparation and chromatographic MS detection). Moreover, we describe clinical applications and performance evaluation of these techniques.",
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Advances and challenges in neurochemical profiling of biological samples using mass spectrometry coupled with separation methods. / Lee, Wonwoong; Park, Na Hyun; Lee, Yongchan; Kim, Ki Hyun; Hong, Jongki.

In: TrAC - Trends in Analytical Chemistry, Vol. 106, 01.09.2018, p. 159-168.

Research output: Contribution to journalReview article

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