Aerosol-to-Hydrosol Sampling and Simultaneous Enrichment of Airborne Bacteria for Rapid Biosensing

Hyeong Rae Kim, Sanggwon An, Jungho Hwang

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Rapid monitoring of biological particulate matter (Bio-PM, bioaerosols) requires an enrichment technique for concentrating the Bio-PM dispersed in the air into a small volume of liquid. In this study, an electrostatic air sampler is employed to capture aerosolized test bacteria in a carrier liquid (aerosol-to-hydrosol (ATH) enrichment). Simultaneously, the captured bacteria are carried into a fluid channel for hydrosol-to-hydrosol (HTH) enrichment with Concanavalin A coated magnetic particles (CMPs). The ATH enrichment capacity of the air sampler was evaluated with an aerosol particle counter for the following test bacteria: Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Acinetobacter baumannii. Then, the HTH enrichment capacity for the ATH-collected sample was evaluated using the colony-counting method, scanning electron microscopy based image analysis, fluorescence microscopy, electrical current measurements, and real-time quantitative polymerase chain reaction (qPCR). The ATH and HTH enrichment capacities for the given operation conditions were up to 80 »000 and 14.9, respectively, resulting in a total enrichment capacity of up to 1.192 × 106. Given that air-to-liquid enrichment required to prepare detectable bacterial samples for real-time qPCR in field environments is of the order of at least 106, our method can be used to prepare a detectable sample from low-concentration airborne bacteria in the field and significantly reduce the time required for Bio-PM monitoring because of its enrichment capacity.

Original languageEnglish
Pages (from-to)2763-2771
Number of pages9
JournalACS Sensors
Volume5
Issue number9
DOIs
Publication statusPublished - 2020 Sep 25

Bibliographical note

Funding Information:
This work was supported by the Bio-Nano Health-Guard Research Center funded by the Ministry of Science, ICT & Future Planning (MSIP) of Korea as a Global Frontier Project (Grant Number HGUARD_2013M3A6B2078959).

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes

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