AFM/EC nano probing of single cells and organelles

R. Fasching, W. H. Ryu, S. J. Bai, J. S. Park, T. Fabian, J. Moseley, A. Grossman, F. Prinz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We have developed a single cell/organelle platform to provide experimental access and control over the electrochemical reactions involved in metabolic pathways of biological microorganisms. As sensing elements we have been using unique needle shaped Atomic Force Microscope (AFM) probes with integrated single or dual electrochemical (EC) nano electrodes. The probe system is designed for local probing of the cytosolic cell environment and cell organelles using amperometric, potentiometric and impedance spectroscopic methods. The sharpness of the probe tip is less than 30nm and allows for a reliable penetration of cell membranes. Reliable immobilization of living single cells and full control of the chemical micro environment close to the single cell has been achieved by introducing a open micro channel systems. Furthermore, this probing set up significantly reduces parasitic electrical capacitances and allows for current detection in sub pico-ampere regime at high signal band widths. Fabrication strategies of the AFM/EC nano probe system as well as open channel micro fluidic systems for single cell/organelle probing will be introduced. Further, the results of using this platform to explore in situ energy conversion reactions in single algae cells and chloroplast and their potential for bio energy sources will be presented and discussed.)

Original languageEnglish
Title of host publicationThe 6th IEEE Conference on SENSORS, IEEE SENSORS 2007
Pages699-702
Number of pages4
DOIs
Publication statusPublished - 2007 Dec 1
Event6th IEEE Conference on SENSORS, IEEE SENSORS 2007 - Atlanta, GA, United States
Duration: 2007 Oct 282007 Oct 31

Publication series

NameProceedings of IEEE Sensors

Other

Other6th IEEE Conference on SENSORS, IEEE SENSORS 2007
CountryUnited States
CityAtlanta, GA
Period07/10/2807/10/31

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Cite this

Fasching, R., Ryu, W. H., Bai, S. J., Park, J. S., Fabian, T., Moseley, J., Grossman, A., & Prinz, F. (2007). AFM/EC nano probing of single cells and organelles. In The 6th IEEE Conference on SENSORS, IEEE SENSORS 2007 (pp. 699-702). [4388495] (Proceedings of IEEE Sensors). https://doi.org/10.1109/ICSENS.2007.4388495