Afterhyperpolarization induced by the activation of nicotinic acetylcholine receptors in pelvic ganglion neurons of male rats

Kyu Sang Park, Seung Kuy Cha, Min Jeong Kim, Na Hyun Kim, Joong Woo Lee, Seong Woo Jeong, In Deok Kong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The electrophysiological mechanism underlying afterhyperpolarization induced by the activation of the nicotinic acetylcholine receptor (nAChR) in male rat major pelvic ganglion neurons (MPG) was investigated using a gramicidin-perforated patch clamp and microscopic fluorescence measurement system. Acetylcholine (ACh) induced fast depolarization through the activation of nAChR, followed by a sustained hyperpolarization after the removal of ACh in a dose-dependent manner (10μM to 1mM). ACh increased both intracellular Ca2+ ([Ca2+]i) and Na+ concentrations ([Na+]i) in MPG neurons. The recovery of [Na+]i after the removal of ACh was markedly delayed by ouabain (100μM), an inhibitor of Na+/K+ ATPase. Pretreatment with ouabain blocked ACh-induced hyperpolarization by 67.2±5.4% (n=7). ACh-induced hyperpolarization was partially attenuated by either the chelation of [Ca2+]i with BAPTA/AM (20μM) or the blockade of small-conductance Ca2+-activated K+ channels by apamin (500nM). Taken together, the activation of nAChR increases [Na+]i and [Ca2+]i, which activates Na+/K+ ATPase and Ca2+-activated K+ channels, respectively. Consequently, hyperpolarization occurs after the activation of nAChR in the autonomic pelvic ganglia.

Original languageEnglish
Pages (from-to)167-171
Number of pages5
JournalNeuroscience Letters
Volume482
Issue number2
DOIs
Publication statusPublished - 2010 Sep 1

Fingerprint

Nicotinic Receptors
Ganglia
Acetylcholine
Neurons
Calcium-Activated Potassium Channels
Ouabain
Autonomic Ganglia
Apamin
Gramicidin
Fluorescence

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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title = "Afterhyperpolarization induced by the activation of nicotinic acetylcholine receptors in pelvic ganglion neurons of male rats",
abstract = "The electrophysiological mechanism underlying afterhyperpolarization induced by the activation of the nicotinic acetylcholine receptor (nAChR) in male rat major pelvic ganglion neurons (MPG) was investigated using a gramicidin-perforated patch clamp and microscopic fluorescence measurement system. Acetylcholine (ACh) induced fast depolarization through the activation of nAChR, followed by a sustained hyperpolarization after the removal of ACh in a dose-dependent manner (10μM to 1mM). ACh increased both intracellular Ca2+ ([Ca2+]i) and Na+ concentrations ([Na+]i) in MPG neurons. The recovery of [Na+]i after the removal of ACh was markedly delayed by ouabain (100μM), an inhibitor of Na+/K+ ATPase. Pretreatment with ouabain blocked ACh-induced hyperpolarization by 67.2±5.4{\%} (n=7). ACh-induced hyperpolarization was partially attenuated by either the chelation of [Ca2+]i with BAPTA/AM (20μM) or the blockade of small-conductance Ca2+-activated K+ channels by apamin (500nM). Taken together, the activation of nAChR increases [Na+]i and [Ca2+]i, which activates Na+/K+ ATPase and Ca2+-activated K+ channels, respectively. Consequently, hyperpolarization occurs after the activation of nAChR in the autonomic pelvic ganglia.",
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Afterhyperpolarization induced by the activation of nicotinic acetylcholine receptors in pelvic ganglion neurons of male rats. / Park, Kyu Sang; Cha, Seung Kuy; Kim, Min Jeong; Kim, Na Hyun; Lee, Joong Woo; Jeong, Seong Woo; Kong, In Deok.

In: Neuroscience Letters, Vol. 482, No. 2, 01.09.2010, p. 167-171.

Research output: Contribution to journalArticle

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T1 - Afterhyperpolarization induced by the activation of nicotinic acetylcholine receptors in pelvic ganglion neurons of male rats

AU - Park, Kyu Sang

AU - Cha, Seung Kuy

AU - Kim, Min Jeong

AU - Kim, Na Hyun

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AU - Jeong, Seong Woo

AU - Kong, In Deok

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