Age-dependent alterations in serum cytokines, peripheral blood mononuclear cell cytokine production, natural killer cell activity, and prostaglandin F

Dong Hee Lee, Minkyung Kim, Minjoo Kim, Young Ju Lee, Hye Jin Yoo, Sang Hyun Lee, Jong Ho Lee

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Abstract

This study aimed to determine age-dependent alterations in serum cytokines, peripheral blood mononuclear cell (PBMC) cytokine production, natural killer (NK) cell activity, and urinary 8-epi-prostaglandin F (PGF). Nine hundred eighty-seven healthy and nonobese subjects were divided into five age groups: 20–34 (group 1), 35–44 (group 2), 45–54 (group 3), 55–64 (group 4), and 65–80 (group 5) years of age. After adjusting for BMI, sex, and smoking and drinking status, serum interferon (IFN)-γ levels decreased in groups 3, 4, and 5 compared with those in groups 1 and 2. Production of IFN-γ by unstimulated PBMCs was lower in the older groups (groups 4 and 5) than in the younger groups (groups 1 and 3). Serum interleukin (IL)-12 was lower in group 5 than in groups 1 and 2. In contrast, both serum and PBMC IL-6 were higher in group 5 than in groups 1, 2, and 3. Urinary 8-epi-PGF increased in group 3 compared with that in group 1 and further increased in group 5. Multiple linear regression analysis revealed that serum IFN-γ levels were negatively affected by age, and NK cell activity at a ratio of E:T = 5:1 was positively affected by PBMC IFN-γ. This study shows the age-related reductions in serum and PBMC IFN-γ and serum IL-12 and age-related increases in serum and PBMC IL-6 and oxidative stress in healthy nonobese subjects. Additionally, circulating IL-6 levels may be a better marker of the chronic low-grade inflammatory activity associated with aging than systemic levels of high-sensitivity C-reactive protein, TNF-α, and IL-1β.

Original languageEnglish
Pages (from-to)1009-1016
Number of pages8
JournalImmunologic Research
Volume65
Issue number5
DOIs
Publication statusPublished - 2017 Oct 1

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Dinoprost
Natural Killer Cells
Blood Cells
Cytokines
Interferons
Serum
Interleukin-6
Interleukin-12
Healthy Volunteers
Interleukin-1
C-Reactive Protein
Drinking
Linear Models
Oxidative Stress
Age Groups
Smoking
Regression Analysis

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Lee, Dong Hee ; Kim, Minkyung ; Kim, Minjoo ; Lee, Young Ju ; Yoo, Hye Jin ; Lee, Sang Hyun ; Lee, Jong Ho. / Age-dependent alterations in serum cytokines, peripheral blood mononuclear cell cytokine production, natural killer cell activity, and prostaglandin F In: Immunologic Research. 2017 ; Vol. 65, No. 5. pp. 1009-1016.
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abstract = "This study aimed to determine age-dependent alterations in serum cytokines, peripheral blood mononuclear cell (PBMC) cytokine production, natural killer (NK) cell activity, and urinary 8-epi-prostaglandin F2α (PGF2α). Nine hundred eighty-seven healthy and nonobese subjects were divided into five age groups: 20–34 (group 1), 35–44 (group 2), 45–54 (group 3), 55–64 (group 4), and 65–80 (group 5) years of age. After adjusting for BMI, sex, and smoking and drinking status, serum interferon (IFN)-γ levels decreased in groups 3, 4, and 5 compared with those in groups 1 and 2. Production of IFN-γ by unstimulated PBMCs was lower in the older groups (groups 4 and 5) than in the younger groups (groups 1 and 3). Serum interleukin (IL)-12 was lower in group 5 than in groups 1 and 2. In contrast, both serum and PBMC IL-6 were higher in group 5 than in groups 1, 2, and 3. Urinary 8-epi-PGF2α increased in group 3 compared with that in group 1 and further increased in group 5. Multiple linear regression analysis revealed that serum IFN-γ levels were negatively affected by age, and NK cell activity at a ratio of E:T = 5:1 was positively affected by PBMC IFN-γ. This study shows the age-related reductions in serum and PBMC IFN-γ and serum IL-12 and age-related increases in serum and PBMC IL-6 and oxidative stress in healthy nonobese subjects. Additionally, circulating IL-6 levels may be a better marker of the chronic low-grade inflammatory activity associated with aging than systemic levels of high-sensitivity C-reactive protein, TNF-α, and IL-1β.",
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Age-dependent alterations in serum cytokines, peripheral blood mononuclear cell cytokine production, natural killer cell activity, and prostaglandin F . / Lee, Dong Hee; Kim, Minkyung; Kim, Minjoo; Lee, Young Ju; Yoo, Hye Jin; Lee, Sang Hyun; Lee, Jong Ho.

In: Immunologic Research, Vol. 65, No. 5, 01.10.2017, p. 1009-1016.

Research output: Contribution to journalArticle

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