Age-related increase in LDL-cholesterol is associated with enhanced oxidative stress and disturbed sphingolipid metabolism

Saem Jung; Minjoo Kim; Hyo Jeong Ryu; Jey Sook Chae; Sang Hyun Lee; Jong Ho Lee

doi:10.1007/s11306-014-0669-3

Age-related increase in LDL-cholesterol is associated with enhanced oxidative stress and disturbed sphingolipid metabolism

Saem Jung, Minjoo Kim, Hyo Jeong Ryu, Jey Sook Chae, Sang Hyun Lee, Jong Ho Lee

Department of Food and Nutrition

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We investigated alterations in plasma metabolites associated with the age-related increase in circulating concentration of low-density lipoprotein (LDL)-cholesterol. The study included 602 healthy, nondiabetic subjects (aged 30–65 years). Among 393 individuals with LDL-cholesterol within the normal limit at baseline, 56 (14.2 %) developed high fasting LDL-cholesterol levels after 3 years. The 337 subjects that retained normal LDL-cholesterol were matched for age, gender, BMI, and fasting LDL-cholesterol to form the control group (n = 78). At the 3-year follow-up, the high-LDL group showed greater increases in serum total-cholesterol and LDL-cholesterol, plasma oxidized LDL, lipoprotein-associated phospholipase A₂ activity, and urinary 8-epi-prostaglandin F_2α than the control group after adjusting for baseline levels. The high-LDL group also showed significant decreases in sphingomyelin (SM) (d18:0/16:1) and phosphatidylcholine (PC) (18:0/20:4), which were associated with an increases on LDL-cholesterol, and significant increases in palmitic amide and lactosylceramide. Mean changes in the levels of SM (d18:0/16:1), C17 sphinganine, PC (18:0/20:4), and eight lysoPCs containing C16:1, C16:0, C17:0, C18:1, C18:0, C20:4, C20:3, and C22:6 were statistically different between control and high-LDL groups. Overall, the change in ox-LDL positively correlated with changes in LDL-cholesterol, Lp-PLA₂ activity, palmitic amide, oleamide, lysoPCs, and C17 sphinganine, and negatively correlated with changes in SM (d18:0/16:1) (r = −0.501, P < 0.001). An age-related increase in LDL-cholesterol is associated with enhanced oxidative stress and disturbed sphingolipid metabolism.

Original language	English
Pages (from-to)	40-49
Number of pages	10
Journal	Metabolomics
Volume	11
Issue number	1
DOIs	https://doi.org/10.1007/s11306-014-0669-3
Publication status	Published - 2014 Feb

Bibliographical note

Funding Information:
Sample collection and experiments were performed by S. J., M. K., H. J. R., J. S. C., S-H. L., and J. H. L. Data were analyzed by S. J., M. K, and J. H. L. J. H. L. provided the research funding and S. J., M. K., and J. H. L. wrote the manuscript. All of the authors read, commented on, and contributed to the submitted manuscript. No potential conflicts of interest relevant to this article were reported. The authors thank the research volunteers who participated in the studies described in this article and the technical assistants of Yonsei Center for Research Facilities, Yonsei University, for data generation of LC–MS (LTQ Orbitrap (micro-LC)). This research was supported by the Bio and Medical Technology Development Program (NRF-2006-2005306 and NRF-2012M3A9C4048762) and Mid-career Researcher Program (NRF-2010-0015017) of the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning, Republic of Korea.

Publisher Copyright:
© 2014, Springer Science+Business Media New York.

All Science Journal Classification (ASJC) codes

Endocrinology, Diabetes and Metabolism
Biochemistry
Clinical Biochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11306-014-0669-3

Cite this

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title = "Age-related increase in LDL-cholesterol is associated with enhanced oxidative stress and disturbed sphingolipid metabolism",

abstract = "We investigated alterations in plasma metabolites associated with the age-related increase in circulating concentration of low-density lipoprotein (LDL)-cholesterol. The study included 602 healthy, nondiabetic subjects (aged 30–65 years). Among 393 individuals with LDL-cholesterol within the normal limit at baseline, 56 (14.2 %) developed high fasting LDL-cholesterol levels after 3 years. The 337 subjects that retained normal LDL-cholesterol were matched for age, gender, BMI, and fasting LDL-cholesterol to form the control group (n = 78). At the 3-year follow-up, the high-LDL group showed greater increases in serum total-cholesterol and LDL-cholesterol, plasma oxidized LDL, lipoprotein-associated phospholipase A2 activity, and urinary 8-epi-prostaglandin F2α than the control group after adjusting for baseline levels. The high-LDL group also showed significant decreases in sphingomyelin (SM) (d18:0/16:1) and phosphatidylcholine (PC) (18:0/20:4), which were associated with an increases on LDL-cholesterol, and significant increases in palmitic amide and lactosylceramide. Mean changes in the levels of SM (d18:0/16:1), C17 sphinganine, PC (18:0/20:4), and eight lysoPCs containing C16:1, C16:0, C17:0, C18:1, C18:0, C20:4, C20:3, and C22:6 were statistically different between control and high-LDL groups. Overall, the change in ox-LDL positively correlated with changes in LDL-cholesterol, Lp-PLA2 activity, palmitic amide, oleamide, lysoPCs, and C17 sphinganine, and negatively correlated with changes in SM (d18:0/16:1) (r = −0.501, P < 0.001). An age-related increase in LDL-cholesterol is associated with enhanced oxidative stress and disturbed sphingolipid metabolism.",

author = "Saem Jung and Minjoo Kim and Ryu, {Hyo Jeong} and Chae, {Jey Sook} and Lee, {Sang Hyun} and Lee, {Jong Ho}",

note = "Funding Information: Sample collection and experiments were performed by S. J., M. K., H. J. R., J. S. C., S-H. L., and J. H. L. Data were analyzed by S. J., M. K, and J. H. L. J. H. L. provided the research funding and S. J., M. K., and J. H. L. wrote the manuscript. All of the authors read, commented on, and contributed to the submitted manuscript. No potential conflicts of interest relevant to this article were reported. The authors thank the research volunteers who participated in the studies described in this article and the technical assistants of Yonsei Center for Research Facilities, Yonsei University, for data generation of LC–MS (LTQ Orbitrap (micro-LC)). This research was supported by the Bio and Medical Technology Development Program (NRF-2006-2005306 and NRF-2012M3A9C4048762) and Mid-career Researcher Program (NRF-2010-0015017) of the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning, Republic of Korea. Publisher Copyright: {\textcopyright} 2014, Springer Science+Business Media New York.",

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doi = "10.1007/s11306-014-0669-3",

language = "English",

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T1 - Age-related increase in LDL-cholesterol is associated with enhanced oxidative stress and disturbed sphingolipid metabolism

AU - Jung, Saem

AU - Kim, Minjoo

AU - Ryu, Hyo Jeong

AU - Chae, Jey Sook

AU - Lee, Sang Hyun

AU - Lee, Jong Ho

N1 - Funding Information: Sample collection and experiments were performed by S. J., M. K., H. J. R., J. S. C., S-H. L., and J. H. L. Data were analyzed by S. J., M. K, and J. H. L. J. H. L. provided the research funding and S. J., M. K., and J. H. L. wrote the manuscript. All of the authors read, commented on, and contributed to the submitted manuscript. No potential conflicts of interest relevant to this article were reported. The authors thank the research volunteers who participated in the studies described in this article and the technical assistants of Yonsei Center for Research Facilities, Yonsei University, for data generation of LC–MS (LTQ Orbitrap (micro-LC)). This research was supported by the Bio and Medical Technology Development Program (NRF-2006-2005306 and NRF-2012M3A9C4048762) and Mid-career Researcher Program (NRF-2010-0015017) of the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning, Republic of Korea. Publisher Copyright: © 2014, Springer Science+Business Media New York.

PY - 2014/2

Y1 - 2014/2

N2 - We investigated alterations in plasma metabolites associated with the age-related increase in circulating concentration of low-density lipoprotein (LDL)-cholesterol. The study included 602 healthy, nondiabetic subjects (aged 30–65 years). Among 393 individuals with LDL-cholesterol within the normal limit at baseline, 56 (14.2 %) developed high fasting LDL-cholesterol levels after 3 years. The 337 subjects that retained normal LDL-cholesterol were matched for age, gender, BMI, and fasting LDL-cholesterol to form the control group (n = 78). At the 3-year follow-up, the high-LDL group showed greater increases in serum total-cholesterol and LDL-cholesterol, plasma oxidized LDL, lipoprotein-associated phospholipase A2 activity, and urinary 8-epi-prostaglandin F2α than the control group after adjusting for baseline levels. The high-LDL group also showed significant decreases in sphingomyelin (SM) (d18:0/16:1) and phosphatidylcholine (PC) (18:0/20:4), which were associated with an increases on LDL-cholesterol, and significant increases in palmitic amide and lactosylceramide. Mean changes in the levels of SM (d18:0/16:1), C17 sphinganine, PC (18:0/20:4), and eight lysoPCs containing C16:1, C16:0, C17:0, C18:1, C18:0, C20:4, C20:3, and C22:6 were statistically different between control and high-LDL groups. Overall, the change in ox-LDL positively correlated with changes in LDL-cholesterol, Lp-PLA2 activity, palmitic amide, oleamide, lysoPCs, and C17 sphinganine, and negatively correlated with changes in SM (d18:0/16:1) (r = −0.501, P < 0.001). An age-related increase in LDL-cholesterol is associated with enhanced oxidative stress and disturbed sphingolipid metabolism.

AB - We investigated alterations in plasma metabolites associated with the age-related increase in circulating concentration of low-density lipoprotein (LDL)-cholesterol. The study included 602 healthy, nondiabetic subjects (aged 30–65 years). Among 393 individuals with LDL-cholesterol within the normal limit at baseline, 56 (14.2 %) developed high fasting LDL-cholesterol levels after 3 years. The 337 subjects that retained normal LDL-cholesterol were matched for age, gender, BMI, and fasting LDL-cholesterol to form the control group (n = 78). At the 3-year follow-up, the high-LDL group showed greater increases in serum total-cholesterol and LDL-cholesterol, plasma oxidized LDL, lipoprotein-associated phospholipase A2 activity, and urinary 8-epi-prostaglandin F2α than the control group after adjusting for baseline levels. The high-LDL group also showed significant decreases in sphingomyelin (SM) (d18:0/16:1) and phosphatidylcholine (PC) (18:0/20:4), which were associated with an increases on LDL-cholesterol, and significant increases in palmitic amide and lactosylceramide. Mean changes in the levels of SM (d18:0/16:1), C17 sphinganine, PC (18:0/20:4), and eight lysoPCs containing C16:1, C16:0, C17:0, C18:1, C18:0, C20:4, C20:3, and C22:6 were statistically different between control and high-LDL groups. Overall, the change in ox-LDL positively correlated with changes in LDL-cholesterol, Lp-PLA2 activity, palmitic amide, oleamide, lysoPCs, and C17 sphinganine, and negatively correlated with changes in SM (d18:0/16:1) (r = −0.501, P < 0.001). An age-related increase in LDL-cholesterol is associated with enhanced oxidative stress and disturbed sphingolipid metabolism.

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Age-related increase in LDL-cholesterol is associated with enhanced oxidative stress and disturbed sphingolipid metabolism

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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