Agglutination study of poly(allyl-α-D-glucopyranose/styrene) latex particles in the presence of Concanavalin A

Jung Yul Lee, In Woo Cheong, Jung Hyun Kim

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Abstract

Latex agglutination of the glucose-modified latex, which were synthesized by emulsion copolymerization of allyl-modified glucose and styrene monomers by specific binding interactions between Concanavalin A (Con A) and allyl-α-d-glucopyranose, were investigated. The surface of the glucose-modified latex was characterized by dye-partition method and the number of glucose was 1517 per latex particle. The average particle size and the polydispersity index of the latex were 78.3 and 1.005, respectively. Time-evolution adsorption behavior of various concentrations of the Con A and the consequent latex agglutination were studied by UV spectrophotometer at 540 nm and ζ-potential analyzer at the fixed latex concentration of 0.02 wt%. Specific binding between Con A and allyl-α-d-glucopyranose leaded the latex particles to coagulate by decreasing the electrostatic repulsion between the particles and mobility.

Original languageEnglish
Pages (from-to)203-208
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume41
Issue number2-3
DOIs
Publication statusPublished - 2005 Mar 15

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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