Aglycone of Rh4 inhibits melanin synthesis in B16 melanoma cells: Possible involvement of the protein kinase A pathway

Yun Mi Jeong, Won Keun Oh, Tien Lam Tran, Wang Kyun Kim, Sang Hyun Sung, Kyeol Bae, Suman Lee, Jong Hyuk Sung

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21 Citations (Scopus)


To our knowledge, there is no report that directly shows an inhibitory effect of ginsenoside on melanin synthesis in B16 melanoma cells. Hence, we investigated whether the aglycone of Rh4 (A-Rh4) inhibits melanin synthesis in B16 melanoma cells, and determined the mechanism of melanin inhibition. We isolated 12 ginsenoside compounds from leaves of Panax ginseng and tested them in B16 melanoma cells. It significantly reduced melanin content and tyrosinase activity under alpha-melanocyte stimulating hormone- and forskolinstimulated conditions. It significantly reduced the cyclic AMP (cAMP) level in B16 melanoma cells, and this might be responsible for the regulation down of MITF and tyrosinase. Phosphorylation of a downstream molecule, a cAMP response-element binding protein, was significantly decreased according to Western blotting and immunofluorescence assay. These data suggest that A-Rh 4 has an anti-melanogenic effect via the protein kinase A pathway.

Original languageEnglish
Pages (from-to)119-125
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Issue number1
Publication statusPublished - 2013 Feb 14


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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