Agmatine pretreatment protects retinal ganglion cells (RGC-5 cell line) from oxidative stress in vitro

Iizuka Yoko, Samin Hong, Chan Yun Kim, Seung Kab Kim, Gong Je Seong

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Agmatine, 2-(4-aminobutyl)guanidine, has been reported to have neuroprotective effects against various neuronal damages. In this study it was investigated whether agmatine pretreatment rescues the retinal ganglion cells from oxidative injury in vitro. After differentiation of transformed rat retinal ganglion cells (RGC-5 cell line) with staurosporine, agmatine (0.0 to 100.0 μM) pretreatment was performed for 2 hours. Subsequently, they were exposed to hydrogen peroxide (0.0 to 2.5 mM) as an oxidative stress. Cell viability was monitored for up to 48 hours with the lactate dehydrogenase (LDH) assay and apoptosis was examined by the terminal deoxynucleotide transferase-mediated terminal uridine deoxynucleotidyl transferase nick end-labeling (TUNEL) method. As a result, differentiated RGC-5 cells were found to have decreased viability after addition of hydrogen peroxide in a dose-dependent manner. This hydrogen peroxide induced cytotoxicity caused apoptosis characterized by DNA fragmentation. Agmatine pretreatment not only increased cell viability but also attenuated DNA fragmentation. In conclusion, agmatine pretreatment demonstrated neuroprotective effects against oxidative stress induced by hydrogen peroxide in differentiated RGC-5 cells in vitro. This suggests a novel therapeutic strategy rescuing retinal ganglion cells from death caused by oxidative injury.

Original languageEnglish
Pages (from-to)245-250
Number of pages6
JournalBiocell
Volume32
Issue number3
Publication statusPublished - 2008 Dec 1

Fingerprint

Agmatine
Retinal Ganglion Cells
Oxidative Stress
Cell Line
Hydrogen Peroxide
Neuroprotective Agents
DNA Fragmentation
Cell Survival
Apoptosis
Staurosporine
DNA Nucleotidylexotransferase
Uridine
Wounds and Injuries
Transferases
L-Lactate Dehydrogenase
In Vitro Techniques
Cell Death

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Yoko, Iizuka ; Hong, Samin ; Kim, Chan Yun ; Kim, Seung Kab ; Seong, Gong Je. / Agmatine pretreatment protects retinal ganglion cells (RGC-5 cell line) from oxidative stress in vitro. In: Biocell. 2008 ; Vol. 32, No. 3. pp. 245-250.
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Agmatine pretreatment protects retinal ganglion cells (RGC-5 cell line) from oxidative stress in vitro. / Yoko, Iizuka; Hong, Samin; Kim, Chan Yun; Kim, Seung Kab; Seong, Gong Je.

In: Biocell, Vol. 32, No. 3, 01.12.2008, p. 245-250.

Research output: Contribution to journalArticle

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