Agmatine protects cultured retinal ganglion cells from tumor necrosis factor-alpha-induced apoptosis

Samin Hong, chanyun kim, Jongeun Lee, Gong Je Seong

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Aims: We investigated the protective effects of agmatine against tumor necrosis factor (TNF)-α-induced apoptosis in transformed rat retinal ganglion cells (RGC-5 cell line). Main methods: The RGC-5 cells were exposed to 50 ng/mL TNF-α for 48 h with or without presence of 100 μM agmatine as indicated. Cell viability was determined by lactate dehydrogenase (LDH) assay. Double staining with Hoechst 33342 and propidium iodide for morphological analysis was performed. Subsequently, using annexin V assay, the proportion of cells actively undergoing apoptosis was determined. Key findings: After 48 h of exposure to 50 ng/mL TNF-α, 17.00% of RGC-5 cells were lost, as evident by LDH assay. TNF-α-induced RGC-5 cell death was reduced to 8.14% with 100 μM agmatine treatment. This observed cell loss was due to apoptotic cell death, as established by annexin V assay. Significance: Our results reveal that agmatine has neuroprotective effects against TNF-α-induced apoptosis in retinal ganglion cells in vitro.

Original languageEnglish
Pages (from-to)28-32
Number of pages5
JournalLife Sciences
Volume84
Issue number1-2
DOIs
Publication statusPublished - 2009 Jan 2

Fingerprint

Agmatine
Retinal Ganglion Cells
Cultured Cells
Tumor Necrosis Factor-alpha
Apoptosis
Assays
Annexin A5
Cell death
L-Lactate Dehydrogenase
Cell Death
Cells
Propidium
Neuroprotective Agents
Rats
Cell Survival
Staining and Labeling
Cell Line

All Science Journal Classification (ASJC) codes

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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abstract = "Aims: We investigated the protective effects of agmatine against tumor necrosis factor (TNF)-α-induced apoptosis in transformed rat retinal ganglion cells (RGC-5 cell line). Main methods: The RGC-5 cells were exposed to 50 ng/mL TNF-α for 48 h with or without presence of 100 μM agmatine as indicated. Cell viability was determined by lactate dehydrogenase (LDH) assay. Double staining with Hoechst 33342 and propidium iodide for morphological analysis was performed. Subsequently, using annexin V assay, the proportion of cells actively undergoing apoptosis was determined. Key findings: After 48 h of exposure to 50 ng/mL TNF-α, 17.00{\%} of RGC-5 cells were lost, as evident by LDH assay. TNF-α-induced RGC-5 cell death was reduced to 8.14{\%} with 100 μM agmatine treatment. This observed cell loss was due to apoptotic cell death, as established by annexin V assay. Significance: Our results reveal that agmatine has neuroprotective effects against TNF-α-induced apoptosis in retinal ganglion cells in vitro.",
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Agmatine protects cultured retinal ganglion cells from tumor necrosis factor-alpha-induced apoptosis. / Hong, Samin; kim, chanyun; Lee, Jongeun; Seong, Gong Je.

In: Life Sciences, Vol. 84, No. 1-2, 02.01.2009, p. 28-32.

Research output: Contribution to journalArticle

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