Agromyces soli sp. nov., isolated from farm soil

Myungjin Lee, Leonid N. Ten, Sung Geun Woo, Joonhong Park

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A Gram-positive, aerobic to microaerophilic, non-motile bacterial strain, designated MJ21 T, was isolated from farm soil and was characterized to determine its taxonomic position by using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain MJ21 T was placed within the genus Agromyces, and exhibited relatively high levels of similarity to Agromyces ulmi XIL01 T (97.8 %), Agromyces aurantiacus YIM 21741 T (97.1 %), Agromyces mediolanus JCM 3346 T (96.7 %), A. mediolanus JCM 1376 (99.1 %), A. mediolanus JCM 9632 (99.1 %), A. mediolanus JCM 9633 (98.9 %) and A. mediolanus JCM 9631 (96.5 %). Chemotaxonomic data also supported the classification of strain MJ21 T within the genus Agromyces. The new isolate contained MK-12 as the predominant menaquinone and rhamnose, galactose and xylose as cell-wall sugars. The major cellular fatty acids (>10 % of the total) were anteiso-C 15: 0, anteiso-C 17: 0 and iso-C 16: 0. Cell-wall amino acids were 2,4-diaminobutyric acid, glutamic acid, glycine and alanine. Diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and one unidentified phospholipid were detected as polar lipids. The DNA G+C content of strain MJ21 T was 73.4 mol%. However, levels of DNA-DNA relatedness between strain MJ21 T and the seven phylogenetically closest Agromyces strains ranged from 14 to 56 %, showing clearly that the new isolate represents a novel genomic species. Strain MJ21 T could be differentiated clearly from its phylogenetic neighbours on the basis of phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ21 T is considered to represent a novel species of the genus Agromyces, for which the name Agromyces soli sp. nov. is proposed. The type strain is MJ21 T (= KCTC 19549 T = JCM 16247 T).

Original languageEnglish
Pages (from-to)1286-1292
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume61
Issue number6
DOIs
Publication statusPublished - 2011 Jun 1

Fingerprint

Agromyces
Agromyces mediolanus
Soil
farm
DNA
farms
Cell Wall
new species
Ulmus
Vitamin K 2
Rhamnose
soil
Phosphatidylglycerols
strain analysis
Cardiolipins
Xylose
acid
Glycolipids
Base Composition
phospholipid

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Lee, Myungjin ; Ten, Leonid N. ; Woo, Sung Geun ; Park, Joonhong. / Agromyces soli sp. nov., isolated from farm soil. In: International Journal of Systematic and Evolutionary Microbiology. 2011 ; Vol. 61, No. 6. pp. 1286-1292.
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abstract = "A Gram-positive, aerobic to microaerophilic, non-motile bacterial strain, designated MJ21 T, was isolated from farm soil and was characterized to determine its taxonomic position by using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain MJ21 T was placed within the genus Agromyces, and exhibited relatively high levels of similarity to Agromyces ulmi XIL01 T (97.8 {\%}), Agromyces aurantiacus YIM 21741 T (97.1 {\%}), Agromyces mediolanus JCM 3346 T (96.7 {\%}), A. mediolanus JCM 1376 (99.1 {\%}), A. mediolanus JCM 9632 (99.1 {\%}), A. mediolanus JCM 9633 (98.9 {\%}) and A. mediolanus JCM 9631 (96.5 {\%}). Chemotaxonomic data also supported the classification of strain MJ21 T within the genus Agromyces. The new isolate contained MK-12 as the predominant menaquinone and rhamnose, galactose and xylose as cell-wall sugars. The major cellular fatty acids (>10 {\%} of the total) were anteiso-C 15: 0, anteiso-C 17: 0 and iso-C 16: 0. Cell-wall amino acids were 2,4-diaminobutyric acid, glutamic acid, glycine and alanine. Diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and one unidentified phospholipid were detected as polar lipids. The DNA G+C content of strain MJ21 T was 73.4 mol{\%}. However, levels of DNA-DNA relatedness between strain MJ21 T and the seven phylogenetically closest Agromyces strains ranged from 14 to 56 {\%}, showing clearly that the new isolate represents a novel genomic species. Strain MJ21 T could be differentiated clearly from its phylogenetic neighbours on the basis of phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ21 T is considered to represent a novel species of the genus Agromyces, for which the name Agromyces soli sp. nov. is proposed. The type strain is MJ21 T (= KCTC 19549 T = JCM 16247 T).",
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Agromyces soli sp. nov., isolated from farm soil. / Lee, Myungjin; Ten, Leonid N.; Woo, Sung Geun; Park, Joonhong.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 61, No. 6, 01.06.2011, p. 1286-1292.

Research output: Contribution to journalArticle

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N2 - A Gram-positive, aerobic to microaerophilic, non-motile bacterial strain, designated MJ21 T, was isolated from farm soil and was characterized to determine its taxonomic position by using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain MJ21 T was placed within the genus Agromyces, and exhibited relatively high levels of similarity to Agromyces ulmi XIL01 T (97.8 %), Agromyces aurantiacus YIM 21741 T (97.1 %), Agromyces mediolanus JCM 3346 T (96.7 %), A. mediolanus JCM 1376 (99.1 %), A. mediolanus JCM 9632 (99.1 %), A. mediolanus JCM 9633 (98.9 %) and A. mediolanus JCM 9631 (96.5 %). Chemotaxonomic data also supported the classification of strain MJ21 T within the genus Agromyces. The new isolate contained MK-12 as the predominant menaquinone and rhamnose, galactose and xylose as cell-wall sugars. The major cellular fatty acids (>10 % of the total) were anteiso-C 15: 0, anteiso-C 17: 0 and iso-C 16: 0. Cell-wall amino acids were 2,4-diaminobutyric acid, glutamic acid, glycine and alanine. Diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and one unidentified phospholipid were detected as polar lipids. The DNA G+C content of strain MJ21 T was 73.4 mol%. However, levels of DNA-DNA relatedness between strain MJ21 T and the seven phylogenetically closest Agromyces strains ranged from 14 to 56 %, showing clearly that the new isolate represents a novel genomic species. Strain MJ21 T could be differentiated clearly from its phylogenetic neighbours on the basis of phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ21 T is considered to represent a novel species of the genus Agromyces, for which the name Agromyces soli sp. nov. is proposed. The type strain is MJ21 T (= KCTC 19549 T = JCM 16247 T).

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