AIMP1 negatively regulates adipogenesis by inhibiting PPARγ

Jong Hyun Kim, Jung Ho Lee, Min Chul Park, Ina Yoon, Kibom Kim, Minji Lee, Hueng Sik Choi, Jung Min Han, Sunghoon Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Adipogenesis is known to be controlled by the concerted actions of transcription factors and co-regulators. However, little is known about the mechanism of regulation of the transcription factors that control adipogenesis. In addition, the adipogenic roles of translational factors remain unclear. Here, we show that aminoacyl tRNA synthetaseinteracting multifunctional protein 1 (AIMP1, also known as p43), an auxiliary factor that is associated with a macromolecular tRNA synthetase complex, negatively regulates adipogenesis through a direct interaction with the DNA-binding domain of peroxisome proliferator-activated receptor γ (PPARγ). We found that AIMP1 expression increases during adipocyte differentiation. Adipogenesis is augmented in AIMP1-deficient cells, as compared with control cells. AIMP1 exhibits high affinity for active PPARγ and interacts with the DNA-binding domain of PPARγ, thereby inhibiting its transcriptional activity. Thus, AIMP1 appears to function as a novel inhibitor of PPARγ that regulates adipocyte differentiation by preventing the transcriptional activation of PPARγ.

Original languageEnglish
Pages (from-to)4483-4493
Number of pages11
JournalJournal of cell science
Volume127
Issue number20
DOIs
Publication statusPublished - 2014 Jan 1

All Science Journal Classification (ASJC) codes

  • Cell Biology

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    Kim, J. H., Lee, J. H., Park, M. C., Yoon, I., Kim, K., Lee, M., Choi, H. S., Han, J. M., & Kim, S. (2014). AIMP1 negatively regulates adipogenesis by inhibiting PPARγ. Journal of cell science, 127(20), 4483-4493. https://doi.org/10.1242/jcs.154930