AIMP1/p43 downregulates TGF-β signaling via stabilization of smurf2

Yeon Sook Lee, Jung Min Han, Sung Hwa Son, Jin Woo Choi, Eun Ju Jeon, Suk Chul Bae, Young In Park, Sunghoon Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

AIMP1 (also known as p43) is a factor associated with a macromolecular aminoacyl-tRNA synthetase (ARS) complex but also plays diverse regulatory roles in various physiological processes. Here, we report that AIMP1 negatively regulates TGF-β signaling via stabilization of Smurf2. TGF-β-dependent phosphorylation and nuclear localization of R-Smads, induction of target genes, and growth arrest were increased in AIMP1-deficient or -suppressed cells. In AIMP1-deficient or suppressed cells, the Smurf2 level was decreased. Various binding assays demonstrated the direction interaction of the C-terminal region of AIMP1 directly with the Smad7-binding region of Smurf2. The association of Smurf2 with Smad7 and its ubiquitination were inhibited by AIMP1, thereby protecting its autocatalytic degradation stimulated by Smad7. Thus, this work suggests the novel activity of AIMP1 as a component of negative feedback loop of TGF-β signaling.

Original languageEnglish
Pages (from-to)395-400
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume371
Issue number3
DOIs
Publication statusPublished - 2008 Jul 4

Fingerprint

Down-Regulation
Stabilization
Physiological Phenomena
Amino Acyl-tRNA Synthetases
Phosphorylation
Ubiquitination
Assays
Genes
Association reactions
Feedback
Degradation
Growth
Direction compound

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Lee, Yeon Sook ; Han, Jung Min ; Son, Sung Hwa ; Choi, Jin Woo ; Jeon, Eun Ju ; Bae, Suk Chul ; Park, Young In ; Kim, Sunghoon. / AIMP1/p43 downregulates TGF-β signaling via stabilization of smurf2. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 371, No. 3. pp. 395-400.
@article{098d9da0284740b6b5501cfec7d21de4,
title = "AIMP1/p43 downregulates TGF-β signaling via stabilization of smurf2",
abstract = "AIMP1 (also known as p43) is a factor associated with a macromolecular aminoacyl-tRNA synthetase (ARS) complex but also plays diverse regulatory roles in various physiological processes. Here, we report that AIMP1 negatively regulates TGF-β signaling via stabilization of Smurf2. TGF-β-dependent phosphorylation and nuclear localization of R-Smads, induction of target genes, and growth arrest were increased in AIMP1-deficient or -suppressed cells. In AIMP1-deficient or suppressed cells, the Smurf2 level was decreased. Various binding assays demonstrated the direction interaction of the C-terminal region of AIMP1 directly with the Smad7-binding region of Smurf2. The association of Smurf2 with Smad7 and its ubiquitination were inhibited by AIMP1, thereby protecting its autocatalytic degradation stimulated by Smad7. Thus, this work suggests the novel activity of AIMP1 as a component of negative feedback loop of TGF-β signaling.",
author = "Lee, {Yeon Sook} and Han, {Jung Min} and Son, {Sung Hwa} and Choi, {Jin Woo} and Jeon, {Eun Ju} and Bae, {Suk Chul} and Park, {Young In} and Sunghoon Kim",
year = "2008",
month = "7",
day = "4",
doi = "10.1016/j.bbrc.2008.04.099",
language = "English",
volume = "371",
pages = "395--400",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

AIMP1/p43 downregulates TGF-β signaling via stabilization of smurf2. / Lee, Yeon Sook; Han, Jung Min; Son, Sung Hwa; Choi, Jin Woo; Jeon, Eun Ju; Bae, Suk Chul; Park, Young In; Kim, Sunghoon.

In: Biochemical and Biophysical Research Communications, Vol. 371, No. 3, 04.07.2008, p. 395-400.

Research output: Contribution to journalArticle

TY - JOUR

T1 - AIMP1/p43 downregulates TGF-β signaling via stabilization of smurf2

AU - Lee, Yeon Sook

AU - Han, Jung Min

AU - Son, Sung Hwa

AU - Choi, Jin Woo

AU - Jeon, Eun Ju

AU - Bae, Suk Chul

AU - Park, Young In

AU - Kim, Sunghoon

PY - 2008/7/4

Y1 - 2008/7/4

N2 - AIMP1 (also known as p43) is a factor associated with a macromolecular aminoacyl-tRNA synthetase (ARS) complex but also plays diverse regulatory roles in various physiological processes. Here, we report that AIMP1 negatively regulates TGF-β signaling via stabilization of Smurf2. TGF-β-dependent phosphorylation and nuclear localization of R-Smads, induction of target genes, and growth arrest were increased in AIMP1-deficient or -suppressed cells. In AIMP1-deficient or suppressed cells, the Smurf2 level was decreased. Various binding assays demonstrated the direction interaction of the C-terminal region of AIMP1 directly with the Smad7-binding region of Smurf2. The association of Smurf2 with Smad7 and its ubiquitination were inhibited by AIMP1, thereby protecting its autocatalytic degradation stimulated by Smad7. Thus, this work suggests the novel activity of AIMP1 as a component of negative feedback loop of TGF-β signaling.

AB - AIMP1 (also known as p43) is a factor associated with a macromolecular aminoacyl-tRNA synthetase (ARS) complex but also plays diverse regulatory roles in various physiological processes. Here, we report that AIMP1 negatively regulates TGF-β signaling via stabilization of Smurf2. TGF-β-dependent phosphorylation and nuclear localization of R-Smads, induction of target genes, and growth arrest were increased in AIMP1-deficient or -suppressed cells. In AIMP1-deficient or suppressed cells, the Smurf2 level was decreased. Various binding assays demonstrated the direction interaction of the C-terminal region of AIMP1 directly with the Smad7-binding region of Smurf2. The association of Smurf2 with Smad7 and its ubiquitination were inhibited by AIMP1, thereby protecting its autocatalytic degradation stimulated by Smad7. Thus, this work suggests the novel activity of AIMP1 as a component of negative feedback loop of TGF-β signaling.

UR - http://www.scopus.com/inward/record.url?scp=43749097417&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43749097417&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2008.04.099

DO - 10.1016/j.bbrc.2008.04.099

M3 - Article

VL - 371

SP - 395

EP - 400

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -