Airborne allergens induce protease activated receptor-2-mediated production of inflammatory cytokines in human gingival epithelium

Ga Yeon Son, Aran Son, Yu Mi Yang, Wonse Park, Inik Chang, Jae Ho Lee, DongMin Shin

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Abstract

Objective In reaching the airways inhaled allergens pass through and contact with the oral mucosa. Although they are often responsible for initiating asthmatic attacks, it is unknown whether airborne allergens can also trigger chronic inflammation of gingival epithelial cells leading to chronic periodontitis. In this study, we investigated the inflammatory responses of human gingival epithelial cells (HGECs) to airborne allergens, particularly German cockroach extract (GCE) with a focus on calcium signaling. Design HGECs isolated from healthy donors were stimulated with GCE. Intracellular Ca 2+ concentration ([Ca 2+ ] i ) was measured with Fura-2-acetoxymethyl ester (Fura-2/AM) staining. Expression of inflammatory cytokines interleukin (IL)-8, IL-1β, IL-6, and NOD-like receptor family, pyridine domain-containing (NLRP) 3 was analyzed using reverse transcription-polymerase chain reaction (RT-PCR). Results GCE promoted increase in the [Ca 2+ ] i in a dose-dependent manner. Depletion of endoplasmic reticulum (ER) Ca 2+ by the ER Ca 2+ ATPase inhibitor thapsigargin (Tg) but not the depletion of extracellular Ca 2+ abolished the GCE-induced increase in [Ca 2+ ] i . Treatment of phospholipase C (PLC) inhibitor (U73122) or 1,4,5-trisinositolphosphate (IP 3 ) receptor inhibitor (2-APB) also prevented GCE-induced increase in [Ca 2+ ] i . Protease activated receptor (PAR)-2 activation mainly mediated the GCE-induced increase in [Ca 2+ ] i and enhanced the expression of IL-8, NLRP3, IL-1β, and IL-6 in HGECs. Conclusions GCE activates PAR-2, which can induce PLC/IP 3 -dependent Ca 2+ signaling pathway, ultimately triggering inflammation via the production of pro-inflammatory cytokines such as IL-1β, IL-6, IL-8, and NLRP 3 in HGECs.

Original languageEnglish
Pages (from-to)138-143
Number of pages6
JournalArchives of Oral Biology
Volume61
DOIs
Publication statusPublished - 2016 Jan 1

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All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

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