Airway reconstruction with carrier-free cell sheets composed of autologous nasal squamous epithelium

Jaseok P. Koo, Chang-Hoon Kim, Jeung Gweon Lee, Kyung Su Kim, Joo Heon Yoon

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

OBJECTIVES/HYPOTHESIS: Although skin has been the most effective graft material for reconstructing the airway lumen, the use of squamous epithelium has many problems. If autologous airway squamous epithelium could differentiate into mucociliary epithelium after in vivo grafting, it could be an answer to these problems. In this study, we wanted to examine whether carrier-free nasal epithelial cell sheets composed of autologous squamous epithelium could be used as a substitute for skin in airway luminal reconstruction in three maxillectomy patients. STUDY DESIGN: In vitro biochemical experiments with in vivo applications. METHODS: We cultured nasal squamous epithelium from three maxillary cancer patients prior to maxillecotmy. These squamous cell sheets were grafted on the forearm free flap, and, after maxillectomy, the surgical defect was reconstructed with a prefabricated myocutaneous radial forearm free flap with the cultured nasal squamous epithelium. At 1 and 3 month intervals after the reconstructive surgery, the cultured cell grafted area was investigated with histologic phenotype, comparing the skin grafted area. RESULTS: The autologous nasal squamous epithelial cell sheet differentiated into mucociliary epithelium without the crust or mucus stagnation that is usually observed in cases in which skin graft is used for airway reconstruction. CONCLUSIONS: We suggest that autologous cultured nasal squamous epithelium, which differentiates into mucociliary epithelium after in vivo grafting, can be used as a clinically relevant substitute for skin graft in airway luminal reconstruction.

Original languageEnglish
Pages (from-to)1750-1755
Number of pages6
JournalLaryngoscope
Volume117
Issue number10
DOIs
Publication statusPublished - 2007 Oct 1

Fingerprint

Nasal Mucosa
Epithelium
Artificial Skin
Free Tissue Flaps
Epithelial Cells
Transplants
Nose
Forearm
Skin
Reconstructive Surgical Procedures
Mucus
Cultured Cells
Phenotype
Neoplasms

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Koo, Jaseok P. ; Kim, Chang-Hoon ; Lee, Jeung Gweon ; Kim, Kyung Su ; Yoon, Joo Heon. / Airway reconstruction with carrier-free cell sheets composed of autologous nasal squamous epithelium. In: Laryngoscope. 2007 ; Vol. 117, No. 10. pp. 1750-1755.
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Airway reconstruction with carrier-free cell sheets composed of autologous nasal squamous epithelium. / Koo, Jaseok P.; Kim, Chang-Hoon; Lee, Jeung Gweon; Kim, Kyung Su; Yoon, Joo Heon.

In: Laryngoscope, Vol. 117, No. 10, 01.10.2007, p. 1750-1755.

Research output: Contribution to journalArticle

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