Airway surface liquid depth measured in ex vivo fragments of pig and human trachea: Dependence on Na+ and Cl- channel function

Yuanlin Song, Wan Namkung, Dennis W. Nielson, Jae Woo Lee, Walter E. Finkbeiner, A. S. Verkman

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The airway surface liquid (ASL) is the thin fluid layer lining the airways whose depth may be reduced in cystic fibrosis. Prior measurements of ASL depth have been made in airway epithelial cell cultures. Here, we established methodology to measure ASL depth to ∼1-μm accuracy in ex vivo fragments of freshly obtained human and pig tracheas. Airway fragments were mounted in chambers designed for perfusion of the basal surface and observation of the apical, fluorescently stained ASL by scanning confocal microscopy using a high numerical aperture lens immersed in perfluorocarbon. Measurement accuracy was verified using standards of specified fluid thickness. ASL depth in well-differentiated primary cultures of human nasal respiratory epithelium was 8.0 ± 0.5 μm (SE 10 cultures) under basal conditions, 8.4 ± 0.4 μm following ENaC inhibition by amiloride, and 14.5 ± 1.2 μm following CFTR stimulation by cAMP agonists. ASL depth in human trachea was 7.0 ± 0.7 μm under basal conditions, 11.0 ± 1.7 μm following amiloride, 17.0 ± 3.4 μm following cAMP agonists, and 7.1 ± 0.5 μm after CFTR inhibition. Similar results were found in pig trachea. This study provides the first direct measurements of ASL depth in intact human airways and indicates the involvement of ENaC sodium channels and CFTR chloride channels in determining ASL depth. We suggest that CF lung disease may be caused by the inability of CFTR-deficient airways to increase their ASL depth transiently following secretory stimuli that in non-CF airways produce transient increases in ASL depth.

Original languageEnglish
Pages (from-to)L1131-L1140
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume297
Issue number6
DOIs
Publication statusPublished - 2009 Dec 1

Fingerprint

Trachea
Swine
Amiloride
Fluorocarbons
Respiratory Mucosa
Chloride Channels
Nasal Mucosa
Sodium Channels
Cystic Fibrosis
Confocal Microscopy
Lenses
Lung Diseases
Cell Culture Techniques
Perfusion
Epithelial Cells
Observation

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

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title = "Airway surface liquid depth measured in ex vivo fragments of pig and human trachea: Dependence on Na+ and Cl- channel function",
abstract = "The airway surface liquid (ASL) is the thin fluid layer lining the airways whose depth may be reduced in cystic fibrosis. Prior measurements of ASL depth have been made in airway epithelial cell cultures. Here, we established methodology to measure ASL depth to ∼1-μm accuracy in ex vivo fragments of freshly obtained human and pig tracheas. Airway fragments were mounted in chambers designed for perfusion of the basal surface and observation of the apical, fluorescently stained ASL by scanning confocal microscopy using a high numerical aperture lens immersed in perfluorocarbon. Measurement accuracy was verified using standards of specified fluid thickness. ASL depth in well-differentiated primary cultures of human nasal respiratory epithelium was 8.0 ± 0.5 μm (SE 10 cultures) under basal conditions, 8.4 ± 0.4 μm following ENaC inhibition by amiloride, and 14.5 ± 1.2 μm following CFTR stimulation by cAMP agonists. ASL depth in human trachea was 7.0 ± 0.7 μm under basal conditions, 11.0 ± 1.7 μm following amiloride, 17.0 ± 3.4 μm following cAMP agonists, and 7.1 ± 0.5 μm after CFTR inhibition. Similar results were found in pig trachea. This study provides the first direct measurements of ASL depth in intact human airways and indicates the involvement of ENaC sodium channels and CFTR chloride channels in determining ASL depth. We suggest that CF lung disease may be caused by the inability of CFTR-deficient airways to increase their ASL depth transiently following secretory stimuli that in non-CF airways produce transient increases in ASL depth.",
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Airway surface liquid depth measured in ex vivo fragments of pig and human trachea : Dependence on Na+ and Cl- channel function. / Song, Yuanlin; Namkung, Wan; Nielson, Dennis W.; Lee, Jae Woo; Finkbeiner, Walter E.; Verkman, A. S.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 297, No. 6, 01.12.2009, p. L1131-L1140.

Research output: Contribution to journalArticle

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T2 - Dependence on Na+ and Cl- channel function

AU - Song, Yuanlin

AU - Namkung, Wan

AU - Nielson, Dennis W.

AU - Lee, Jae Woo

AU - Finkbeiner, Walter E.

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