AKAP12 mediates barrier functions of fibrotic scars during CNS repair

Jong Ho Cha, Hee Jun Wee, Ji Hae Seo, Bum Ju Ahn, Ji Hyeon Park, Jun Mo Yang, Sae Won Lee, Eun Hee Kim, Ok Hee Lee, Ji Hoe Heo, Hyo Jong Lee, Irwin H. Gelman, Ken Arai, Eng H. Lo, Kyu Won Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The repair process after CNS injury shows a well-organized cascade of three distinct stages: inflammation, new tissue formation, and remodeling. In the new tissue formation stage, various cells migrate and form the fibrotic scar surrounding the lesion site. The fibrotic scar is known as an obstacle for axonal regeneration in the remodeling stage. However, the role of the fibrotic scar in the new tissue formation stage remains largely unknown. We found that the number of A-kinase anchoring protein 12 (AKAP12)-positive cells in the fibrotic scar was increased over time, and the cells formed a structure which traps various immune cells. Furthermore, the AKAP12-positive cells strongly express junction proteins which enable the structure to function as a physical barrier. In in vivo validation, AKAP12 knock-out (KO) mice showed leakage from a lesion, resulting from an impaired structure with the loss of the junction complex. Consistently, focal brain injury in the AKAP12 KO mice led to extended inflammation and more severe tissue damage compared to the wild type (WT) mice. Accordingly, our results suggest that AKAP12-positive cells in the fibrotic scar may restrict excessive inflammation, demonstrating certain mechanisms that could underlie the beneficial actions of the fibrotic scar in the new tissue formation stage during the CNS repair process.

Original languageEnglish
Article numbere94695
JournalPloS one
Volume9
Issue number4
DOIs
Publication statusPublished - 2014 Apr 23

Fingerprint

protein kinases
Protein Kinases
Cicatrix
Repair
Phosphotransferases
Tissue
Proteins
inflammation
Cells
cells
Inflammation
Knockout Mice
lesions (animal)
mice
Architectural Accessibility
Brain Injuries
Regeneration
Brain
traps
tissues

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Cha, J. H., Wee, H. J., Seo, J. H., Ahn, B. J., Park, J. H., Yang, J. M., ... Kim, K. W. (2014). AKAP12 mediates barrier functions of fibrotic scars during CNS repair. PloS one, 9(4), [e94695]. https://doi.org/10.1371/journal.pone.0094695
Cha, Jong Ho ; Wee, Hee Jun ; Seo, Ji Hae ; Ahn, Bum Ju ; Park, Ji Hyeon ; Yang, Jun Mo ; Lee, Sae Won ; Kim, Eun Hee ; Lee, Ok Hee ; Heo, Ji Hoe ; Lee, Hyo Jong ; Gelman, Irwin H. ; Arai, Ken ; Lo, Eng H. ; Kim, Kyu Won. / AKAP12 mediates barrier functions of fibrotic scars during CNS repair. In: PloS one. 2014 ; Vol. 9, No. 4.
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abstract = "The repair process after CNS injury shows a well-organized cascade of three distinct stages: inflammation, new tissue formation, and remodeling. In the new tissue formation stage, various cells migrate and form the fibrotic scar surrounding the lesion site. The fibrotic scar is known as an obstacle for axonal regeneration in the remodeling stage. However, the role of the fibrotic scar in the new tissue formation stage remains largely unknown. We found that the number of A-kinase anchoring protein 12 (AKAP12)-positive cells in the fibrotic scar was increased over time, and the cells formed a structure which traps various immune cells. Furthermore, the AKAP12-positive cells strongly express junction proteins which enable the structure to function as a physical barrier. In in vivo validation, AKAP12 knock-out (KO) mice showed leakage from a lesion, resulting from an impaired structure with the loss of the junction complex. Consistently, focal brain injury in the AKAP12 KO mice led to extended inflammation and more severe tissue damage compared to the wild type (WT) mice. Accordingly, our results suggest that AKAP12-positive cells in the fibrotic scar may restrict excessive inflammation, demonstrating certain mechanisms that could underlie the beneficial actions of the fibrotic scar in the new tissue formation stage during the CNS repair process.",
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Cha, JH, Wee, HJ, Seo, JH, Ahn, BJ, Park, JH, Yang, JM, Lee, SW, Kim, EH, Lee, OH, Heo, JH, Lee, HJ, Gelman, IH, Arai, K, Lo, EH & Kim, KW 2014, 'AKAP12 mediates barrier functions of fibrotic scars during CNS repair', PloS one, vol. 9, no. 4, e94695. https://doi.org/10.1371/journal.pone.0094695

AKAP12 mediates barrier functions of fibrotic scars during CNS repair. / Cha, Jong Ho; Wee, Hee Jun; Seo, Ji Hae; Ahn, Bum Ju; Park, Ji Hyeon; Yang, Jun Mo; Lee, Sae Won; Kim, Eun Hee; Lee, Ok Hee; Heo, Ji Hoe; Lee, Hyo Jong; Gelman, Irwin H.; Arai, Ken; Lo, Eng H.; Kim, Kyu Won.

In: PloS one, Vol. 9, No. 4, e94695, 23.04.2014.

Research output: Contribution to journalArticle

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AU - Cha, Jong Ho

AU - Wee, Hee Jun

AU - Seo, Ji Hae

AU - Ahn, Bum Ju

AU - Park, Ji Hyeon

AU - Yang, Jun Mo

AU - Lee, Sae Won

AU - Kim, Eun Hee

AU - Lee, Ok Hee

AU - Heo, Ji Hoe

AU - Lee, Hyo Jong

AU - Gelman, Irwin H.

AU - Arai, Ken

AU - Lo, Eng H.

AU - Kim, Kyu Won

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Y1 - 2014/4/23

N2 - The repair process after CNS injury shows a well-organized cascade of three distinct stages: inflammation, new tissue formation, and remodeling. In the new tissue formation stage, various cells migrate and form the fibrotic scar surrounding the lesion site. The fibrotic scar is known as an obstacle for axonal regeneration in the remodeling stage. However, the role of the fibrotic scar in the new tissue formation stage remains largely unknown. We found that the number of A-kinase anchoring protein 12 (AKAP12)-positive cells in the fibrotic scar was increased over time, and the cells formed a structure which traps various immune cells. Furthermore, the AKAP12-positive cells strongly express junction proteins which enable the structure to function as a physical barrier. In in vivo validation, AKAP12 knock-out (KO) mice showed leakage from a lesion, resulting from an impaired structure with the loss of the junction complex. Consistently, focal brain injury in the AKAP12 KO mice led to extended inflammation and more severe tissue damage compared to the wild type (WT) mice. Accordingly, our results suggest that AKAP12-positive cells in the fibrotic scar may restrict excessive inflammation, demonstrating certain mechanisms that could underlie the beneficial actions of the fibrotic scar in the new tissue formation stage during the CNS repair process.

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Cha JH, Wee HJ, Seo JH, Ahn BJ, Park JH, Yang JM et al. AKAP12 mediates barrier functions of fibrotic scars during CNS repair. PloS one. 2014 Apr 23;9(4). e94695. https://doi.org/10.1371/journal.pone.0094695