Akt is involved in the inhibition of cell proliferation by EGF

Hoo Jeon Soung, Woo Jeong Jeong, Jae Young Cho, Kee Ho Lee, Kang Yell Choi

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Abstract

Axin is a negative regulator of the Wnt/β-catenin pathway and is involved in the regulation of axis formation and proliferation. Involvement of Axin in the regulation of other signaling pathways is poorly understood. In this study, we investigated the involvement of Akt in growth regulation by Axin in L929 fibroblasts stimulated by EGF. Akt activity was increased by EGF treatment and Ras activation, respectively. Both the EGF-and Ras-induced Akt activations were abolished by Axin induction, as revealed by both Western blot and immunocytochemical analyses. The proliferation and Akt activation induced by EGF were decreased by Axin induction, and the effects of EGF were abolished by treatment of an Akt-specific inhibitor. Therefore, Axin inhibits EGF-induced proliferation of L929 fibroblasts by blocking Akt activation.

Original languageEnglish
Pages (from-to)491-498
Number of pages8
JournalExperimental and Molecular Medicine
Volume39
Issue number4
Publication statusPublished - 2007 Aug 31

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Genetics

Cite this

Soung, H. J., Jeong, W. J., Cho, J. Y., Lee, K. H., & Choi, K. Y. (2007). Akt is involved in the inhibition of cell proliferation by EGF. Experimental and Molecular Medicine, 39(4), 491-498.