Akt is involved in the inhibition of cell proliferation by EGF

Hoo Jeon Soung, Woo Jeong Jeong, Jae Young Cho, Kee Ho Lee, Kang Yell Choi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Axin is a negative regulator of the Wnt/β-catenin pathway and is involved in the regulation of axis formation and proliferation. Involvement of Axin in the regulation of other signaling pathways is poorly understood. In this study, we investigated the involvement of Akt in growth regulation by Axin in L929 fibroblasts stimulated by EGF. Akt activity was increased by EGF treatment and Ras activation, respectively. Both the EGF-and Ras-induced Akt activations were abolished by Axin induction, as revealed by both Western blot and immunocytochemical analyses. The proliferation and Akt activation induced by EGF were decreased by Axin induction, and the effects of EGF were abolished by treatment of an Akt-specific inhibitor. Therefore, Axin inhibits EGF-induced proliferation of L929 fibroblasts by blocking Akt activation.

Original languageEnglish
Pages (from-to)491-498
Number of pages8
JournalExperimental and Molecular Medicine
Volume39
Issue number4
Publication statusPublished - 2007 Aug 31

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Cell proliferation
Epidermal Growth Factor
Cell Proliferation
Chemical activation
Fibroblasts
Catenins
Wnt Signaling Pathway
Western Blotting
Growth

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Genetics

Cite this

Soung, H. J., Jeong, W. J., Cho, J. Y., Lee, K. H., & Choi, K. Y. (2007). Akt is involved in the inhibition of cell proliferation by EGF. Experimental and Molecular Medicine, 39(4), 491-498.
Soung, Hoo Jeon ; Jeong, Woo Jeong ; Cho, Jae Young ; Lee, Kee Ho ; Choi, Kang Yell. / Akt is involved in the inhibition of cell proliferation by EGF. In: Experimental and Molecular Medicine. 2007 ; Vol. 39, No. 4. pp. 491-498.
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Soung, HJ, Jeong, WJ, Cho, JY, Lee, KH & Choi, KY 2007, 'Akt is involved in the inhibition of cell proliferation by EGF', Experimental and Molecular Medicine, vol. 39, no. 4, pp. 491-498.

Akt is involved in the inhibition of cell proliferation by EGF. / Soung, Hoo Jeon; Jeong, Woo Jeong; Cho, Jae Young; Lee, Kee Ho; Choi, Kang Yell.

In: Experimental and Molecular Medicine, Vol. 39, No. 4, 31.08.2007, p. 491-498.

Research output: Contribution to journalArticle

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T1 - Akt is involved in the inhibition of cell proliferation by EGF

AU - Soung, Hoo Jeon

AU - Jeong, Woo Jeong

AU - Cho, Jae Young

AU - Lee, Kee Ho

AU - Choi, Kang Yell

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AB - Axin is a negative regulator of the Wnt/β-catenin pathway and is involved in the regulation of axis formation and proliferation. Involvement of Axin in the regulation of other signaling pathways is poorly understood. In this study, we investigated the involvement of Akt in growth regulation by Axin in L929 fibroblasts stimulated by EGF. Akt activity was increased by EGF treatment and Ras activation, respectively. Both the EGF-and Ras-induced Akt activations were abolished by Axin induction, as revealed by both Western blot and immunocytochemical analyses. The proliferation and Akt activation induced by EGF were decreased by Axin induction, and the effects of EGF were abolished by treatment of an Akt-specific inhibitor. Therefore, Axin inhibits EGF-induced proliferation of L929 fibroblasts by blocking Akt activation.

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