Alginate hydrogel embedding poly(D,L-lactide-co-glycolide) porous scaffold disks for cartilage tissue engineering

Sung Wook Choi, Seung Kwan Moon, Ji Yeon Chu, Hye Won Lee, Tae Joon Park, Jung Hyun Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Small poly(D,L-lactide-co-glycolide) scaffold disks (2 mm in diameter) with a bimodal porous structure of 237.5±34.2 μm for large pores and 9.2±3.9 μm for micropores were prepared by gas-forming and controlled-pre-cipitation methods. Chondrocyte-seeded scaffold disks were dispersed in an alginate solution containing culture media. The dispersion was transferred into a Teflon mold with a specific shape, eventually forming an alginate hydrogel (approximately 1 cm in dimension) that contains cell/disk aggregates after crosslinking using CaCl 2. In vitro assessment of the alginate hydrogel showed significant increases in DNA and glycosaminoglycan content after 21 days, suggesting that chondrocyte still proliferated on the small scaffold disks in the alginate hydrogel despite its large dimensions. Moreover, in vivo immunohistochemical analysis of the alginate hydrogel demonstrated that chon-drocytes secreted native extracellular matrix proteins (e.g., collagen, proteoglycan). We believe that our approach of using alginate hydrogel containing cell/disk aggregates with a shape fitted to the target site could provide a versatile platform to engineer large and thick tissues and/or organs.

Original languageEnglish
Pages (from-to)447-452
Number of pages6
JournalMacromolecular Research
Volume20
Issue number5
DOIs
Publication statusPublished - 2012 May 1

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All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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