Abstract
An all-purpose multifunctional optical microscope based on digital holographic microscopy (DHM) is proposed, which enables DHM to be practically suitable for bioimaging applications. With our simple suggested digital signal-processing scheme, we have demonstrated that various optical images, including pure amplitude, pure phase, dark-field, phase-contrast, and differential interference contrast images, can be obtained from a single DHM measurement. Because these images obtained with our method are essentially the same as those obtainable with conventional optical imaging instruments, biologists can easily analyze them with their previous knowledge and experience.
| Original language | English |
|---|---|
| Article number | 087001 |
| Journal | Optical Engineering |
| Volume | 47 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2008 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Atomic and Molecular Physics, and Optics
- Engineering(all)
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All-in-one multifunctional optical microscope with a single holographic measurement. / Liu, Cheng; Bae, Yoon Sung; Yang, Wenzhong; Kim, Dug Young.
In: Optical Engineering, Vol. 47, No. 8, 087001, 01.12.2008.Research output: Contribution to journal › Article
TY - JOUR
T1 - All-in-one multifunctional optical microscope with a single holographic measurement
AU - Liu, Cheng
AU - Bae, Yoon Sung
AU - Yang, Wenzhong
AU - Kim, Dug Young
PY - 2008/12/1
Y1 - 2008/12/1
N2 - An all-purpose multifunctional optical microscope based on digital holographic microscopy (DHM) is proposed, which enables DHM to be practically suitable for bioimaging applications. With our simple suggested digital signal-processing scheme, we have demonstrated that various optical images, including pure amplitude, pure phase, dark-field, phase-contrast, and differential interference contrast images, can be obtained from a single DHM measurement. Because these images obtained with our method are essentially the same as those obtainable with conventional optical imaging instruments, biologists can easily analyze them with their previous knowledge and experience.
AB - An all-purpose multifunctional optical microscope based on digital holographic microscopy (DHM) is proposed, which enables DHM to be practically suitable for bioimaging applications. With our simple suggested digital signal-processing scheme, we have demonstrated that various optical images, including pure amplitude, pure phase, dark-field, phase-contrast, and differential interference contrast images, can be obtained from a single DHM measurement. Because these images obtained with our method are essentially the same as those obtainable with conventional optical imaging instruments, biologists can easily analyze them with their previous knowledge and experience.
UR - http://www.scopus.com/inward/record.url?scp=77957077519&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77957077519&partnerID=8YFLogxK
U2 - 10.1117/1.2968708
DO - 10.1117/1.2968708
M3 - Article
AN - SCOPUS:77957077519
VL - 47
JO - Optical Engineering
JF - Optical Engineering
SN - 0091-3286
IS - 8
M1 - 087001
ER -