An all-purpose multifunctional optical microscope based on digital holographic microscopy (DHM) is proposed, which enables DHM to be practically suitable for bioimaging applications. With our simple suggested digital signal-processing scheme, we have demonstrated that various optical images, including pure amplitude, pure phase, dark-field, phase-contrast, and differential interference contrast images, can be obtained from a single DHM measurement. Because these images obtained with our method are essentially the same as those obtainable with conventional optical imaging instruments, biologists can easily analyze them with their previous knowledge and experience.
Bibliographical noteFunding Information:
This work was supported by the Creative Research Initiatives (CRI) Program of the Korea Science and Engineering Foundation (KOSEF), Ministry of Science and Technology (MOST).
All Science Journal Classification (ASJC) codes
- Atomic and Molecular Physics, and Optics