Allergenic characterization of a novel allergen, homologous to chymotrypsin, from German cockroach

Kyoung Yong Jeong, Mina Son, Jae Hyun Lee, Chein Soo Hong, Jung Won Park

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purpose: Cockroach feces are known to be rich in IgE-reactive components. Various protease allergens were identified by proteomic analysis of German cockroach fecal extract in a previous study. In this study, we characterized a novel allergen, a chymotrypsin-like serine protease. Methods: A cDNA sequence homologous to chymotrypsin was obtained by analysis of German cockroach expressed sequence tag (EST) clones. The recombinant chymotrypsins from the German cockroach and house dust mite (Der f 6) were expressed in Escherichia coli using the pEXP5NT/TOPO vector system, and their allergenicity was investigated by ELISA. Results: The deduced amino acid sequence of German cockroach chymotrypsin showed 32.7 to 43.1% identity with mite group 3 (trypsin) and group 6 (chymotrypsin) allergens. Sera from 8 of 28 German cockroach allergy subjects (28.6%) showed IgE binding to the recombinant protein. IgE binding to the recombinant cockroach chymotrypsin was inhibited by house dust mite chymotrypsin Der f 6, while it minimally inhibited the German cockroach whole body extract. Conclusions: A novel allergen homologous to chymotrypsin was identified from the German cockroach and was cross-reactive with Der f 6.

Original languageEnglish
Pages (from-to)283-289
Number of pages7
JournalAllergy, Asthma and Immunology Research
Volume7
Issue number3
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

Blattellidae
Chymotrypsin
Allergens
Immunoglobulin E
Pyroglyphidae
Cockroaches
Chymases
Mites
Expressed Sequence Tags
Serine Proteases
Sequence Homology
Recombinant Proteins
Feces
Proteomics
Trypsin
Amino Acid Sequence
Hypersensitivity
Peptide Hydrolases
Complementary DNA
Clone Cells

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pulmonary and Respiratory Medicine

Cite this

Jeong, Kyoung Yong ; Son, Mina ; Lee, Jae Hyun ; Hong, Chein Soo ; Park, Jung Won. / Allergenic characterization of a novel allergen, homologous to chymotrypsin, from German cockroach. In: Allergy, Asthma and Immunology Research. 2015 ; Vol. 7, No. 3. pp. 283-289.
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abstract = "Purpose: Cockroach feces are known to be rich in IgE-reactive components. Various protease allergens were identified by proteomic analysis of German cockroach fecal extract in a previous study. In this study, we characterized a novel allergen, a chymotrypsin-like serine protease. Methods: A cDNA sequence homologous to chymotrypsin was obtained by analysis of German cockroach expressed sequence tag (EST) clones. The recombinant chymotrypsins from the German cockroach and house dust mite (Der f 6) were expressed in Escherichia coli using the pEXP5NT/TOPO vector system, and their allergenicity was investigated by ELISA. Results: The deduced amino acid sequence of German cockroach chymotrypsin showed 32.7 to 43.1{\%} identity with mite group 3 (trypsin) and group 6 (chymotrypsin) allergens. Sera from 8 of 28 German cockroach allergy subjects (28.6{\%}) showed IgE binding to the recombinant protein. IgE binding to the recombinant cockroach chymotrypsin was inhibited by house dust mite chymotrypsin Der f 6, while it minimally inhibited the German cockroach whole body extract. Conclusions: A novel allergen homologous to chymotrypsin was identified from the German cockroach and was cross-reactive with Der f 6.",
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Allergenic characterization of a novel allergen, homologous to chymotrypsin, from German cockroach. / Jeong, Kyoung Yong; Son, Mina; Lee, Jae Hyun; Hong, Chein Soo; Park, Jung Won.

In: Allergy, Asthma and Immunology Research, Vol. 7, No. 3, 01.01.2015, p. 283-289.

Research output: Contribution to journalArticle

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