Allergenicity of recombinant profilins from Japanese hop, Humulus japonicus

K. Y. Jeong, I. S. Han, S. Y. Choi, J. H. Lee, J. S. Lee, C. S. Hong, J. W. Park

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Pollen from Japanese hop, Humulus japonicus, is a major cause of pollinosis in Korea. Profilin (15 kDa) from Humulus scandens has been associated with strong allergenicity in allergic Chinese patients. Profilin has also been detected in pollen extract from Korean Japanese hop by proteomic analysis and immunoglobulin (Ig) E immunoblotting. However, the allergenicity of allergens isolated from Japanese hop has not been investigated in Korean individuals. This study was undertaken to produce recombinant profilin from Japanese hop and evaluate its allergenicity. Methods: Complementary DNA sequences encoding 2 isoallergens were cloned by reverse transcription polymerase chain reaction and their recombinant proteins expressed in Escherichia coli. The IgE-binding reactivities of the recombinant allergens were assessed by enzymelinked immunosorbent assay. Results: The deduced amino acid sequences of the H japonicus profilins were 68.7% to 80.2% homologous with profilins from mugwort (Art v 4), ragweed (Amb a 14), and birch (Bet v 2). Two isoallergens of profilin from H japonicus were 78.2% identical. Notably, the cDNA sequences of these 2 isoallergens were 98.5% (AY268422) and 98.7% (AY268424) identical to those of H scandens. Serum samples from Japanese hop-sensitized individuals showed 12.9% IgE reactivity to both of the recombinant profilin isoallergens from H japonicus, indicating that profilin may not be an allergenically dominant component of Japanese hop pollen. The recombinant profilins showed only 0% to 9.3% inhibition of the crude extract. Conclusions: Two isoallergens of profilin that are highly conserved with those of mugwort, ragweed, and birch were identified in H japonicus. Profilins from Japanese hop pollen may play a minor role in the pathogenesis of pollinosis in Koreans.

Original languageEnglish
Pages (from-to)345-350
Number of pages6
JournalJournal of Investigational Allergology and Clinical Immunology
Volume23
Issue number5
Publication statusPublished - 2013 Aug 12

Fingerprint

Profilins
Humulus
Pollen
Immunoglobulin E
Ambrosia
Artemisia
Betula
Seasonal Allergic Rhinitis
Allergens
Complementary DNA
Immunosorbents
Art
Korea
Complex Mixtures
Recombinant Proteins
Immunoblotting
Proteomics
Reverse Transcription
Amino Acid Sequence

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Jeong, K. Y., Han, I. S., Choi, S. Y., Lee, J. H., Lee, J. S., Hong, C. S., & Park, J. W. (2013). Allergenicity of recombinant profilins from Japanese hop, Humulus japonicus. Journal of Investigational Allergology and Clinical Immunology, 23(5), 345-350.
Jeong, K. Y. ; Han, I. S. ; Choi, S. Y. ; Lee, J. H. ; Lee, J. S. ; Hong, C. S. ; Park, J. W. / Allergenicity of recombinant profilins from Japanese hop, Humulus japonicus. In: Journal of Investigational Allergology and Clinical Immunology. 2013 ; Vol. 23, No. 5. pp. 345-350.
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abstract = "Pollen from Japanese hop, Humulus japonicus, is a major cause of pollinosis in Korea. Profilin (15 kDa) from Humulus scandens has been associated with strong allergenicity in allergic Chinese patients. Profilin has also been detected in pollen extract from Korean Japanese hop by proteomic analysis and immunoglobulin (Ig) E immunoblotting. However, the allergenicity of allergens isolated from Japanese hop has not been investigated in Korean individuals. This study was undertaken to produce recombinant profilin from Japanese hop and evaluate its allergenicity. Methods: Complementary DNA sequences encoding 2 isoallergens were cloned by reverse transcription polymerase chain reaction and their recombinant proteins expressed in Escherichia coli. The IgE-binding reactivities of the recombinant allergens were assessed by enzymelinked immunosorbent assay. Results: The deduced amino acid sequences of the H japonicus profilins were 68.7{\%} to 80.2{\%} homologous with profilins from mugwort (Art v 4), ragweed (Amb a 14), and birch (Bet v 2). Two isoallergens of profilin from H japonicus were 78.2{\%} identical. Notably, the cDNA sequences of these 2 isoallergens were 98.5{\%} (AY268422) and 98.7{\%} (AY268424) identical to those of H scandens. Serum samples from Japanese hop-sensitized individuals showed 12.9{\%} IgE reactivity to both of the recombinant profilin isoallergens from H japonicus, indicating that profilin may not be an allergenically dominant component of Japanese hop pollen. The recombinant profilins showed only 0{\%} to 9.3{\%} inhibition of the crude extract. Conclusions: Two isoallergens of profilin that are highly conserved with those of mugwort, ragweed, and birch were identified in H japonicus. Profilins from Japanese hop pollen may play a minor role in the pathogenesis of pollinosis in Koreans.",
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Allergenicity of recombinant profilins from Japanese hop, Humulus japonicus. / Jeong, K. Y.; Han, I. S.; Choi, S. Y.; Lee, J. H.; Lee, J. S.; Hong, C. S.; Park, J. W.

In: Journal of Investigational Allergology and Clinical Immunology, Vol. 23, No. 5, 12.08.2013, p. 345-350.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Allergenicity of recombinant profilins from Japanese hop, Humulus japonicus

AU - Jeong, K. Y.

AU - Han, I. S.

AU - Choi, S. Y.

AU - Lee, J. H.

AU - Lee, J. S.

AU - Hong, C. S.

AU - Park, J. W.

PY - 2013/8/12

Y1 - 2013/8/12

N2 - Pollen from Japanese hop, Humulus japonicus, is a major cause of pollinosis in Korea. Profilin (15 kDa) from Humulus scandens has been associated with strong allergenicity in allergic Chinese patients. Profilin has also been detected in pollen extract from Korean Japanese hop by proteomic analysis and immunoglobulin (Ig) E immunoblotting. However, the allergenicity of allergens isolated from Japanese hop has not been investigated in Korean individuals. This study was undertaken to produce recombinant profilin from Japanese hop and evaluate its allergenicity. Methods: Complementary DNA sequences encoding 2 isoallergens were cloned by reverse transcription polymerase chain reaction and their recombinant proteins expressed in Escherichia coli. The IgE-binding reactivities of the recombinant allergens were assessed by enzymelinked immunosorbent assay. Results: The deduced amino acid sequences of the H japonicus profilins were 68.7% to 80.2% homologous with profilins from mugwort (Art v 4), ragweed (Amb a 14), and birch (Bet v 2). Two isoallergens of profilin from H japonicus were 78.2% identical. Notably, the cDNA sequences of these 2 isoallergens were 98.5% (AY268422) and 98.7% (AY268424) identical to those of H scandens. Serum samples from Japanese hop-sensitized individuals showed 12.9% IgE reactivity to both of the recombinant profilin isoallergens from H japonicus, indicating that profilin may not be an allergenically dominant component of Japanese hop pollen. The recombinant profilins showed only 0% to 9.3% inhibition of the crude extract. Conclusions: Two isoallergens of profilin that are highly conserved with those of mugwort, ragweed, and birch were identified in H japonicus. Profilins from Japanese hop pollen may play a minor role in the pathogenesis of pollinosis in Koreans.

AB - Pollen from Japanese hop, Humulus japonicus, is a major cause of pollinosis in Korea. Profilin (15 kDa) from Humulus scandens has been associated with strong allergenicity in allergic Chinese patients. Profilin has also been detected in pollen extract from Korean Japanese hop by proteomic analysis and immunoglobulin (Ig) E immunoblotting. However, the allergenicity of allergens isolated from Japanese hop has not been investigated in Korean individuals. This study was undertaken to produce recombinant profilin from Japanese hop and evaluate its allergenicity. Methods: Complementary DNA sequences encoding 2 isoallergens were cloned by reverse transcription polymerase chain reaction and their recombinant proteins expressed in Escherichia coli. The IgE-binding reactivities of the recombinant allergens were assessed by enzymelinked immunosorbent assay. Results: The deduced amino acid sequences of the H japonicus profilins were 68.7% to 80.2% homologous with profilins from mugwort (Art v 4), ragweed (Amb a 14), and birch (Bet v 2). Two isoallergens of profilin from H japonicus were 78.2% identical. Notably, the cDNA sequences of these 2 isoallergens were 98.5% (AY268422) and 98.7% (AY268424) identical to those of H scandens. Serum samples from Japanese hop-sensitized individuals showed 12.9% IgE reactivity to both of the recombinant profilin isoallergens from H japonicus, indicating that profilin may not be an allergenically dominant component of Japanese hop pollen. The recombinant profilins showed only 0% to 9.3% inhibition of the crude extract. Conclusions: Two isoallergens of profilin that are highly conserved with those of mugwort, ragweed, and birch were identified in H japonicus. Profilins from Japanese hop pollen may play a minor role in the pathogenesis of pollinosis in Koreans.

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