Allergenicity of recombinant troponin C from Tyrophagus putrescentiae

Kyoung Yong Jeong, Chung Ryul Kim, Sunjin Un, Myung Hee Yi, In Yong Lee, Jung Won Park, Chein Soo Hong, Tai Soon Yong

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: The storage mite, Tyrophagus putrescentiae, produces potent allergens, many of which have not been characterized. This study was undertaken to characterize the allergenicity of troponin C from T. putrescentiae. Methods: A cDNA encoding 17.7 kDa troponin C, with homology to cockroach allergen Bla g 6, was identified from T. putrescentiae-expressed sequence tags. Recombinant troponin C was expressed and IgE responses to the recombinant protein were assessed in the presence and absence of 10 mM CaCl2. Cross-reactivity between T. putrescentiae troponin C and Bla g 6 was tested using an inhibition ELISA. Results: Recombinant T. putrescentiae troponin C shares 62.7-85.5% homology with troponin C from various arthropods. Sera from 5 of 47 subjects in our study group (10.6%) showed IgE binding to the recombinant protein. Interestingly, addition of 10 mM CaCl2 increased the intensity of IgE binding approximately 2-fold. In an immune-inhibition ELISA with these sera, T. putrescetiae troponin C and Bla g 6 did not cross-react significantly. Conclusions: Troponin C is a new mite allergen with calcium-dependent IgE reactivity.

Original languageEnglish
Pages (from-to)207-213
Number of pages7
JournalInternational Archives of Allergy and Immunology
Volume151
Issue number3
DOIs
Publication statusPublished - 2010 Feb 1

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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