Allosteric interaction of a herpes simplex viral thymidine kinase with host DNA polymerase α in mouse LP1-1 cells

C. G. Kim, E. Y. Shim, J. E. Lee, Yeun Kyu Jang, C. G. Lee, S. D. Park

Research output: Contribution to journalArticle

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Abstract

A DNA polymerase α-associated multienzyme complex isolated from mouse LP1-1 cells transfected with the thymidine kinase gene of herpes simplex virus type I (1) showed activities of DNA polymerase α, topoisomerase II, and thymidine kinase (TK) in the complex. TK antiserum recognized a 43 kDa polypeptide only in the fraction of the multienzyme complex prepared from the LP1-1 cells but not that from L-M(TK - ) cells. In permeabilized cells, hydroxyurea did not show any inhibitory effect on either DNA polymerase or TK, whereas aphidicolin, novobiocin and TK antiserum inhibited both enzymes. These results provide evidence for the functional association and an allosteric interaction between the viral TK and host DNA polymerase α.

Original languageEnglish
Pages (from-to)651-657
Number of pages7
JournalBiochemistry and Molecular Biology International
Volume32
Issue number4
Publication statusPublished - 1994 Jan 1

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Herpes Simplex
Thymidine Kinase
DNA-Directed DNA Polymerase
Multienzyme Complexes
Immune Sera
DNA Polymerase II
Polynucleotide 5'-Hydroxyl-Kinase
Aphidicolin
Novobiocin
Type II DNA Topoisomerase
Hydroxyurea
Human Herpesvirus 1
Viruses
Genes
Peptides
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

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abstract = "A DNA polymerase α-associated multienzyme complex isolated from mouse LP1-1 cells transfected with the thymidine kinase gene of herpes simplex virus type I (1) showed activities of DNA polymerase α, topoisomerase II, and thymidine kinase (TK) in the complex. TK antiserum recognized a 43 kDa polypeptide only in the fraction of the multienzyme complex prepared from the LP1-1 cells but not that from L-M(TK - ) cells. In permeabilized cells, hydroxyurea did not show any inhibitory effect on either DNA polymerase or TK, whereas aphidicolin, novobiocin and TK antiserum inhibited both enzymes. These results provide evidence for the functional association and an allosteric interaction between the viral TK and host DNA polymerase α.",
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Allosteric interaction of a herpes simplex viral thymidine kinase with host DNA polymerase α in mouse LP1-1 cells. / Kim, C. G.; Shim, E. Y.; Lee, J. E.; Jang, Yeun Kyu; Lee, C. G.; Park, S. D.

In: Biochemistry and Molecular Biology International, Vol. 32, No. 4, 01.01.1994, p. 651-657.

Research output: Contribution to journalArticle

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