Alpha B-crystallin prevents the arrhythmogenic effects of particulate matter isolated from ambient air by attenuating oxidative stress

Hyelim Park, Sanghoon Park, Hyunju Jeon, Byeong Wook Song, Jin Bae Kim, Chang Soo Kim, Hui Nam Pak, Ki Chul Hwang, Moon Hyoung Lee, Ji Hyung Chung, Boyoung Joung

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Ca2+/calmodulin-dependent protein kinase II (CaMKII) is activated by particulate matter (PM) isolated from ambient air and linked to prolonged repolarization and cardiac arrhythmia. We evaluated whether alpha B-crystallin (CryAB), a heat shock protein, could prevent the arrhythmogenic effects of PM by preventing CaMKII activation. CryAB was delivered into cardiac cells using a TAT-protein transduction domain (TAT-CryAB). ECGs were measured before and after tracheal exposure of diesel exhaust particles (DEP) and each intervention in adult Sprague-Dawley rats. After endotracheal exposure of DEP (200μg/mL for 30minutes, n=11), QT intervals were prolonged from 115±14ms to 144±20ms (p=0.03), and premature ventricular contractions were observed more frequently (0% vs. 44%) than control (n=5) and TAT-Cry (n=5). However, DEP-induced arrhythmia was not observed in TAT-CryAB (1mg/kg) pretreated rats (n=5). In optical mapping of Langendorff-perfused rat heats, compared with baseline, DEP infusion of 12.5μg/mL (n=12) increased apicobasal action potential duration (APD) differences from 2±6ms to 36±15ms (p<0.001), APD restitution slope from 0.26±0.07 to 1.19±0.11 (p<0.001) and ventricular tachycardia (VT) from 0% to 75% (p<0.001). DEP infusion easily induced spatially discordant alternans. However, the effects of DEP were prevented by TAT-CryAB (1mg/kg, n=9). In rat myocytes, while DEP increased reactive oxygen species (ROS) generation and phosphated CaMKII, TAT-CryAB prevented these effects. In conclusion, CryAB, a small heat shock protein, might prevent the arrhythmogenic effects of PM by attenuating ROS generation and CaMKII activation.

Original languageEnglish
Pages (from-to)267-275
Number of pages9
JournalToxicology and Applied Pharmacology
Volume266
Issue number2
DOIs
Publication statusPublished - 2013 Jan 5

Fingerprint

alpha-Crystallin B Chain
Vehicle Emissions
Oxidative stress
Particulate Matter
Oxidative Stress
Air
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Rats
Action Potentials
Cardiac Arrhythmias
Reactive Oxygen Species
Chemical activation
Small Heat-Shock Proteins
Ventricular Premature Complexes
Ventricular Tachycardia
Heat-Shock Proteins
Electrocardiography
Muscle Cells
Sprague Dawley Rats
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pharmacology

Cite this

Park, Hyelim ; Park, Sanghoon ; Jeon, Hyunju ; Song, Byeong Wook ; Kim, Jin Bae ; Kim, Chang Soo ; Pak, Hui Nam ; Hwang, Ki Chul ; Lee, Moon Hyoung ; Chung, Ji Hyung ; Joung, Boyoung. / Alpha B-crystallin prevents the arrhythmogenic effects of particulate matter isolated from ambient air by attenuating oxidative stress. In: Toxicology and Applied Pharmacology. 2013 ; Vol. 266, No. 2. pp. 267-275.
@article{70e342accb794216b25d46b087c86317,
title = "Alpha B-crystallin prevents the arrhythmogenic effects of particulate matter isolated from ambient air by attenuating oxidative stress",
abstract = "Ca2+/calmodulin-dependent protein kinase II (CaMKII) is activated by particulate matter (PM) isolated from ambient air and linked to prolonged repolarization and cardiac arrhythmia. We evaluated whether alpha B-crystallin (CryAB), a heat shock protein, could prevent the arrhythmogenic effects of PM by preventing CaMKII activation. CryAB was delivered into cardiac cells using a TAT-protein transduction domain (TAT-CryAB). ECGs were measured before and after tracheal exposure of diesel exhaust particles (DEP) and each intervention in adult Sprague-Dawley rats. After endotracheal exposure of DEP (200μg/mL for 30minutes, n=11), QT intervals were prolonged from 115±14ms to 144±20ms (p=0.03), and premature ventricular contractions were observed more frequently (0{\%} vs. 44{\%}) than control (n=5) and TAT-Cry (n=5). However, DEP-induced arrhythmia was not observed in TAT-CryAB (1mg/kg) pretreated rats (n=5). In optical mapping of Langendorff-perfused rat heats, compared with baseline, DEP infusion of 12.5μg/mL (n=12) increased apicobasal action potential duration (APD) differences from 2±6ms to 36±15ms (p<0.001), APD restitution slope from 0.26±0.07 to 1.19±0.11 (p<0.001) and ventricular tachycardia (VT) from 0{\%} to 75{\%} (p<0.001). DEP infusion easily induced spatially discordant alternans. However, the effects of DEP were prevented by TAT-CryAB (1mg/kg, n=9). In rat myocytes, while DEP increased reactive oxygen species (ROS) generation and phosphated CaMKII, TAT-CryAB prevented these effects. In conclusion, CryAB, a small heat shock protein, might prevent the arrhythmogenic effects of PM by attenuating ROS generation and CaMKII activation.",
author = "Hyelim Park and Sanghoon Park and Hyunju Jeon and Song, {Byeong Wook} and Kim, {Jin Bae} and Kim, {Chang Soo} and Pak, {Hui Nam} and Hwang, {Ki Chul} and Lee, {Moon Hyoung} and Chung, {Ji Hyung} and Boyoung Joung",
year = "2013",
month = "1",
day = "5",
doi = "10.1016/j.taap.2012.10.013",
language = "English",
volume = "266",
pages = "267--275",
journal = "Toxicology and Applied Pharmacology",
issn = "0041-008X",
publisher = "Academic Press Inc.",
number = "2",

}

Alpha B-crystallin prevents the arrhythmogenic effects of particulate matter isolated from ambient air by attenuating oxidative stress. / Park, Hyelim; Park, Sanghoon; Jeon, Hyunju; Song, Byeong Wook; Kim, Jin Bae; Kim, Chang Soo; Pak, Hui Nam; Hwang, Ki Chul; Lee, Moon Hyoung; Chung, Ji Hyung; Joung, Boyoung.

In: Toxicology and Applied Pharmacology, Vol. 266, No. 2, 05.01.2013, p. 267-275.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Alpha B-crystallin prevents the arrhythmogenic effects of particulate matter isolated from ambient air by attenuating oxidative stress

AU - Park, Hyelim

AU - Park, Sanghoon

AU - Jeon, Hyunju

AU - Song, Byeong Wook

AU - Kim, Jin Bae

AU - Kim, Chang Soo

AU - Pak, Hui Nam

AU - Hwang, Ki Chul

AU - Lee, Moon Hyoung

AU - Chung, Ji Hyung

AU - Joung, Boyoung

PY - 2013/1/5

Y1 - 2013/1/5

N2 - Ca2+/calmodulin-dependent protein kinase II (CaMKII) is activated by particulate matter (PM) isolated from ambient air and linked to prolonged repolarization and cardiac arrhythmia. We evaluated whether alpha B-crystallin (CryAB), a heat shock protein, could prevent the arrhythmogenic effects of PM by preventing CaMKII activation. CryAB was delivered into cardiac cells using a TAT-protein transduction domain (TAT-CryAB). ECGs were measured before and after tracheal exposure of diesel exhaust particles (DEP) and each intervention in adult Sprague-Dawley rats. After endotracheal exposure of DEP (200μg/mL for 30minutes, n=11), QT intervals were prolonged from 115±14ms to 144±20ms (p=0.03), and premature ventricular contractions were observed more frequently (0% vs. 44%) than control (n=5) and TAT-Cry (n=5). However, DEP-induced arrhythmia was not observed in TAT-CryAB (1mg/kg) pretreated rats (n=5). In optical mapping of Langendorff-perfused rat heats, compared with baseline, DEP infusion of 12.5μg/mL (n=12) increased apicobasal action potential duration (APD) differences from 2±6ms to 36±15ms (p<0.001), APD restitution slope from 0.26±0.07 to 1.19±0.11 (p<0.001) and ventricular tachycardia (VT) from 0% to 75% (p<0.001). DEP infusion easily induced spatially discordant alternans. However, the effects of DEP were prevented by TAT-CryAB (1mg/kg, n=9). In rat myocytes, while DEP increased reactive oxygen species (ROS) generation and phosphated CaMKII, TAT-CryAB prevented these effects. In conclusion, CryAB, a small heat shock protein, might prevent the arrhythmogenic effects of PM by attenuating ROS generation and CaMKII activation.

AB - Ca2+/calmodulin-dependent protein kinase II (CaMKII) is activated by particulate matter (PM) isolated from ambient air and linked to prolonged repolarization and cardiac arrhythmia. We evaluated whether alpha B-crystallin (CryAB), a heat shock protein, could prevent the arrhythmogenic effects of PM by preventing CaMKII activation. CryAB was delivered into cardiac cells using a TAT-protein transduction domain (TAT-CryAB). ECGs were measured before and after tracheal exposure of diesel exhaust particles (DEP) and each intervention in adult Sprague-Dawley rats. After endotracheal exposure of DEP (200μg/mL for 30minutes, n=11), QT intervals were prolonged from 115±14ms to 144±20ms (p=0.03), and premature ventricular contractions were observed more frequently (0% vs. 44%) than control (n=5) and TAT-Cry (n=5). However, DEP-induced arrhythmia was not observed in TAT-CryAB (1mg/kg) pretreated rats (n=5). In optical mapping of Langendorff-perfused rat heats, compared with baseline, DEP infusion of 12.5μg/mL (n=12) increased apicobasal action potential duration (APD) differences from 2±6ms to 36±15ms (p<0.001), APD restitution slope from 0.26±0.07 to 1.19±0.11 (p<0.001) and ventricular tachycardia (VT) from 0% to 75% (p<0.001). DEP infusion easily induced spatially discordant alternans. However, the effects of DEP were prevented by TAT-CryAB (1mg/kg, n=9). In rat myocytes, while DEP increased reactive oxygen species (ROS) generation and phosphated CaMKII, TAT-CryAB prevented these effects. In conclusion, CryAB, a small heat shock protein, might prevent the arrhythmogenic effects of PM by attenuating ROS generation and CaMKII activation.

UR - http://www.scopus.com/inward/record.url?scp=84871839836&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871839836&partnerID=8YFLogxK

U2 - 10.1016/j.taap.2012.10.013

DO - 10.1016/j.taap.2012.10.013

M3 - Article

C2 - 23153557

AN - SCOPUS:84871839836

VL - 266

SP - 267

EP - 275

JO - Toxicology and Applied Pharmacology

JF - Toxicology and Applied Pharmacology

SN - 0041-008X

IS - 2

ER -