TY - JOUR
T1 - Alteration of expression of Ca2+ signaling proteins and adaptation of Ca2+ signaling in SERCA2+/- mouse parotid acini
AU - Choi, Jong Hoon
AU - Jo, Hae
AU - Hong, Jeong Hee
AU - Lee, Syng Ill
AU - Shin, Dong Min
PY - 2008/4
Y1 - 2008/4
N2 - Purpose: The sarco/endoplasmic reticulum Ca2+-ATPase (SERCA), encoded by ATP2A2, is an essential component for G-protein coupled receptor (GPCR)-dependent Ca2+ signaling. However whether the changes in Ca2+ signaling and Ca2+ signaling proteins in parotid acinor cells are affected by a partial loss of SERCA2 are not known. Materials and Methods: In SERCA2+/- mouse parotid gland acinar cells, Ca2+ signaling, expression levels of Ca2+ signaling proteins, and amylase secretion were investigated. Results: SERCA2+/- mice showed decreased SERCA2 expression and an upregulation of the plasma membrane Ca2+ ATPase. A partial loss of SERCA2 changed the expression level of 1, 4, 5-tris-inositolphosphate receptors (IP3Rs), but the localization and activities of IP3Rs were not altered. In SERCA2+/- mice, muscarinic stimulation resulted in greater amylase release, and the expression of synaptotagimin was increased compared to wild type mice. Conclusion. These results suggest that a partial loss of SERCA2 affects the expression and activity of Ca2+ signaling proteins in the parotid gland acini, however, overall Ca2+ signaling is unchanged.
AB - Purpose: The sarco/endoplasmic reticulum Ca2+-ATPase (SERCA), encoded by ATP2A2, is an essential component for G-protein coupled receptor (GPCR)-dependent Ca2+ signaling. However whether the changes in Ca2+ signaling and Ca2+ signaling proteins in parotid acinor cells are affected by a partial loss of SERCA2 are not known. Materials and Methods: In SERCA2+/- mouse parotid gland acinar cells, Ca2+ signaling, expression levels of Ca2+ signaling proteins, and amylase secretion were investigated. Results: SERCA2+/- mice showed decreased SERCA2 expression and an upregulation of the plasma membrane Ca2+ ATPase. A partial loss of SERCA2 changed the expression level of 1, 4, 5-tris-inositolphosphate receptors (IP3Rs), but the localization and activities of IP3Rs were not altered. In SERCA2+/- mice, muscarinic stimulation resulted in greater amylase release, and the expression of synaptotagimin was increased compared to wild type mice. Conclusion. These results suggest that a partial loss of SERCA2 affects the expression and activity of Ca2+ signaling proteins in the parotid gland acini, however, overall Ca2+ signaling is unchanged.
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U2 - 10.3349/ymj.2008.49.2.311
DO - 10.3349/ymj.2008.49.2.311
M3 - Article
C2 - 18452270
AN - SCOPUS:44649173927
VL - 49
SP - 311
EP - 321
JO - Yonsei Medical Journal
JF - Yonsei Medical Journal
SN - 0513-5796
IS - 2
ER -