Purpose: The sarco/endoplasmic reticulum Ca 2+ -ATPase (SERCA), encoded by ATP2A2, is an essential component for G-protein coupled receptor (GPCR)-dependent Ca 2+ signaling. However whether the changes in Ca 2+ signaling and Ca 2+ signaling proteins in parotid acinor cells are affected by a partial loss of SERCA2 are not known. Materials and Methods: In SERCA2 +/- mouse parotid gland acinar cells, Ca 2+ signaling, expression levels of Ca 2+ signaling proteins, and amylase secretion were investigated. Results: SERCA2 +/- mice showed decreased SERCA2 expression and an upregulation of the plasma membrane Ca 2+ ATPase. A partial loss of SERCA2 changed the expression level of 1, 4, 5-tris-inositolphosphate receptors (IP 3 Rs), but the localization and activities of IP 3 Rs were not altered. In SERCA2 +/- mice, muscarinic stimulation resulted in greater amylase release, and the expression of synaptotagimin was increased compared to wild type mice. Conclusion. These results suggest that a partial loss of SERCA2 affects the expression and activity of Ca 2+ signaling proteins in the parotid gland acini, however, overall Ca 2+ signaling is unchanged.
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