Abstract
Purpose: The sarco/endoplasmic reticulum Ca 2+ -ATPase (SERCA), encoded by ATP2A2, is an essential component for G-protein coupled receptor (GPCR)-dependent Ca 2+ signaling. However whether the changes in Ca 2+ signaling and Ca 2+ signaling proteins in parotid acinor cells are affected by a partial loss of SERCA2 are not known. Materials and Methods: In SERCA2 +/- mouse parotid gland acinar cells, Ca 2+ signaling, expression levels of Ca 2+ signaling proteins, and amylase secretion were investigated. Results: SERCA2 +/- mice showed decreased SERCA2 expression and an upregulation of the plasma membrane Ca 2+ ATPase. A partial loss of SERCA2 changed the expression level of 1, 4, 5-tris-inositolphosphate receptors (IP 3 Rs), but the localization and activities of IP 3 Rs were not altered. In SERCA2 +/- mice, muscarinic stimulation resulted in greater amylase release, and the expression of synaptotagimin was increased compared to wild type mice. Conclusion. These results suggest that a partial loss of SERCA2 affects the expression and activity of Ca 2+ signaling proteins in the parotid gland acini, however, overall Ca 2+ signaling is unchanged.
| Original language | English |
|---|---|
| Pages (from-to) | 311-321 |
| Number of pages | 11 |
| Journal | Yonsei medical journal |
| Volume | 49 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2008 Apr 1 |
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All Science Journal Classification (ASJC) codes
- Medicine(all)
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Alteration of expression of Ca 2+ signaling proteins and adaptation of Ca 2+ signaling in SERCA2 +/- mouse parotid acini . / Choi, Jong Hoon; Jo, Hae; Hong, Jeong Hee; Lee, Syng Ill; Shin, DongMin.
In: Yonsei medical journal, Vol. 49, No. 2, 01.04.2008, p. 311-321.Research output: Contribution to journal › Article
TY - JOUR
T1 - Alteration of expression of Ca 2+ signaling proteins and adaptation of Ca 2+ signaling in SERCA2 +/- mouse parotid acini
AU - Choi, Jong Hoon
AU - Jo, Hae
AU - Hong, Jeong Hee
AU - Lee, Syng Ill
AU - Shin, DongMin
PY - 2008/4/1
Y1 - 2008/4/1
N2 - Purpose: The sarco/endoplasmic reticulum Ca 2+ -ATPase (SERCA), encoded by ATP2A2, is an essential component for G-protein coupled receptor (GPCR)-dependent Ca 2+ signaling. However whether the changes in Ca 2+ signaling and Ca 2+ signaling proteins in parotid acinor cells are affected by a partial loss of SERCA2 are not known. Materials and Methods: In SERCA2 +/- mouse parotid gland acinar cells, Ca 2+ signaling, expression levels of Ca 2+ signaling proteins, and amylase secretion were investigated. Results: SERCA2 +/- mice showed decreased SERCA2 expression and an upregulation of the plasma membrane Ca 2+ ATPase. A partial loss of SERCA2 changed the expression level of 1, 4, 5-tris-inositolphosphate receptors (IP 3 Rs), but the localization and activities of IP 3 Rs were not altered. In SERCA2 +/- mice, muscarinic stimulation resulted in greater amylase release, and the expression of synaptotagimin was increased compared to wild type mice. Conclusion. These results suggest that a partial loss of SERCA2 affects the expression and activity of Ca 2+ signaling proteins in the parotid gland acini, however, overall Ca 2+ signaling is unchanged.
AB - Purpose: The sarco/endoplasmic reticulum Ca 2+ -ATPase (SERCA), encoded by ATP2A2, is an essential component for G-protein coupled receptor (GPCR)-dependent Ca 2+ signaling. However whether the changes in Ca 2+ signaling and Ca 2+ signaling proteins in parotid acinor cells are affected by a partial loss of SERCA2 are not known. Materials and Methods: In SERCA2 +/- mouse parotid gland acinar cells, Ca 2+ signaling, expression levels of Ca 2+ signaling proteins, and amylase secretion were investigated. Results: SERCA2 +/- mice showed decreased SERCA2 expression and an upregulation of the plasma membrane Ca 2+ ATPase. A partial loss of SERCA2 changed the expression level of 1, 4, 5-tris-inositolphosphate receptors (IP 3 Rs), but the localization and activities of IP 3 Rs were not altered. In SERCA2 +/- mice, muscarinic stimulation resulted in greater amylase release, and the expression of synaptotagimin was increased compared to wild type mice. Conclusion. These results suggest that a partial loss of SERCA2 affects the expression and activity of Ca 2+ signaling proteins in the parotid gland acini, however, overall Ca 2+ signaling is unchanged.
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U2 - 10.3349/ymj.2008.49.2.311
DO - 10.3349/ymj.2008.49.2.311
M3 - Article
VL - 49
SP - 311
EP - 321
JO - Yonsei Medical Journal
JF - Yonsei Medical Journal
SN - 0513-5796
IS - 2
ER -