Alteration of gut microbiota in carbapenem-resistant enterobacteriaceae carriers during fecal microbiota transplantation according to decolonization periods

Jin Jae Lee, Dongeun Yong, Ki Tae Suk, Dong Joon Kim, Heung Jeong Woo, Seung Soon Lee, Bong Soo Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Fecal microbiota transplantation (FMT) has been suggested as an alternative therapeutic option to decolonize carbapenem-resistant Enterobacteriaceae (CRE). However, the analysis of gut microbiota alteration in CRE carriers during FMT is still limited. Here, gut microbiota changes in CRE carriers were evaluated during FMT according to decolonization periods. The decolonization of 10 CRE carriers was evaluated after FMT, using serial consecutive rectal swab cultures. Alterations of gut microbiota before and after FMT (56 serial samples) were analyzed using high-throughput sequencing. The decolonization rates of CRE carriers were 40%, 50%, and 90% within 1, 3 and 5 months after initial FMT, respectively. Gut microbiota significantly changed after FMT (p = 0.003). Microbiota alteration was different between the early decolonization carriers (EDC) and late decolonization carriers (LDC). Microbiota convergence in carriers to donors was detected in EDC within 4 weeks, and keystone genera within the Bacteroidetes were found in the gut microbiota of EDC before FMT. The relative abundance of Klebsiella was lower in EDC than in LDC, before and after FMT. Our results indicate that FMT is a potential option for CRE decolonization. The gut microbiota of CRE carriers could be used to predict decolonization timing after FMT, and determine repeated FMT necessity.

Original languageEnglish
Article number352
Pages (from-to)1-15
Number of pages15
JournalMicroorganisms
Volume9
Issue number2
DOIs
Publication statusPublished - 2021 Feb

Bibliographical note

Funding Information:
Funding: This research was funded by Basic Science Research Program (2019R1I1A3A01060465 and 2020R1A6A1A03043026) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Bio & Medical Technology Development Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2017M3A9F3043837).

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Virology
  • Microbiology (medical)

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