Alteration of hTERT full-length variant expression level showed different gene expression profiles and genomic copy number changes in breast cancer

SunYoung Rha, Hei C. Jeung, Woo I. Yang, Jin J. Kim, Tae J. Oh, Sung W. An, Hyuncheol Chung

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We analyzed hTERT splicing patterns with respect to telomerase activity in breast cancer. Using a cDNA microarray in 22 cell lines, we observed the difference in expression profiling based on the different levels of full-length variant expression with 71 selected genes. Using 33 known genes that act with the telomerase complex, we performed unsupervised clustering with all cell lines, and found a clustering tendency related to the full-length variant expression level. Using array-based CGH, highly altered genomic copy number changes were found more often in MCF-7 (159 genes) than in MDA-MB-231 (109 genes) and MDA-MB-435 (49 genes), suggesting more genomic changes in MCF-7 cells. On comparing MCF-7 with MDA-MB231 and MDA-MB-435 cell lines, we identified 8 genes with different copy numbers, including dystroglycan, which is located in the p12-21.2 area of chromosome 3. In conclusion, alterations in the level of the full-length variant of hTERT showed different gene expression profiles and genomic copy number changes in breast cancer, which require further study into their cause-and-effect relationship.

Original languageEnglish
Pages (from-to)749-755
Number of pages7
JournalOncology Reports
Volume15
Issue number4
Publication statusPublished - 2006 Apr 1

Fingerprint

Transcriptome
Breast Neoplasms
Genes
Telomerase
Cell Line
Cluster Analysis
Dystroglycans
Chromosomes, Human, Pair 3
MCF-7 Cells
Oligonucleotide Array Sequence Analysis

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

@article{c29cc485ed734190b51c134100c0f7d1,
title = "Alteration of hTERT full-length variant expression level showed different gene expression profiles and genomic copy number changes in breast cancer",
abstract = "We analyzed hTERT splicing patterns with respect to telomerase activity in breast cancer. Using a cDNA microarray in 22 cell lines, we observed the difference in expression profiling based on the different levels of full-length variant expression with 71 selected genes. Using 33 known genes that act with the telomerase complex, we performed unsupervised clustering with all cell lines, and found a clustering tendency related to the full-length variant expression level. Using array-based CGH, highly altered genomic copy number changes were found more often in MCF-7 (159 genes) than in MDA-MB-231 (109 genes) and MDA-MB-435 (49 genes), suggesting more genomic changes in MCF-7 cells. On comparing MCF-7 with MDA-MB231 and MDA-MB-435 cell lines, we identified 8 genes with different copy numbers, including dystroglycan, which is located in the p12-21.2 area of chromosome 3. In conclusion, alterations in the level of the full-length variant of hTERT showed different gene expression profiles and genomic copy number changes in breast cancer, which require further study into their cause-and-effect relationship.",
author = "SunYoung Rha and Jeung, {Hei C.} and Yang, {Woo I.} and Kim, {Jin J.} and Oh, {Tae J.} and An, {Sung W.} and Hyuncheol Chung",
year = "2006",
month = "4",
day = "1",
language = "English",
volume = "15",
pages = "749--755",
journal = "Oncology Reports",
issn = "1021-335X",
publisher = "Spandidos Publications",
number = "4",

}

Alteration of hTERT full-length variant expression level showed different gene expression profiles and genomic copy number changes in breast cancer. / Rha, SunYoung; Jeung, Hei C.; Yang, Woo I.; Kim, Jin J.; Oh, Tae J.; An, Sung W.; Chung, Hyuncheol.

In: Oncology Reports, Vol. 15, No. 4, 01.04.2006, p. 749-755.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Alteration of hTERT full-length variant expression level showed different gene expression profiles and genomic copy number changes in breast cancer

AU - Rha, SunYoung

AU - Jeung, Hei C.

AU - Yang, Woo I.

AU - Kim, Jin J.

AU - Oh, Tae J.

AU - An, Sung W.

AU - Chung, Hyuncheol

PY - 2006/4/1

Y1 - 2006/4/1

N2 - We analyzed hTERT splicing patterns with respect to telomerase activity in breast cancer. Using a cDNA microarray in 22 cell lines, we observed the difference in expression profiling based on the different levels of full-length variant expression with 71 selected genes. Using 33 known genes that act with the telomerase complex, we performed unsupervised clustering with all cell lines, and found a clustering tendency related to the full-length variant expression level. Using array-based CGH, highly altered genomic copy number changes were found more often in MCF-7 (159 genes) than in MDA-MB-231 (109 genes) and MDA-MB-435 (49 genes), suggesting more genomic changes in MCF-7 cells. On comparing MCF-7 with MDA-MB231 and MDA-MB-435 cell lines, we identified 8 genes with different copy numbers, including dystroglycan, which is located in the p12-21.2 area of chromosome 3. In conclusion, alterations in the level of the full-length variant of hTERT showed different gene expression profiles and genomic copy number changes in breast cancer, which require further study into their cause-and-effect relationship.

AB - We analyzed hTERT splicing patterns with respect to telomerase activity in breast cancer. Using a cDNA microarray in 22 cell lines, we observed the difference in expression profiling based on the different levels of full-length variant expression with 71 selected genes. Using 33 known genes that act with the telomerase complex, we performed unsupervised clustering with all cell lines, and found a clustering tendency related to the full-length variant expression level. Using array-based CGH, highly altered genomic copy number changes were found more often in MCF-7 (159 genes) than in MDA-MB-231 (109 genes) and MDA-MB-435 (49 genes), suggesting more genomic changes in MCF-7 cells. On comparing MCF-7 with MDA-MB231 and MDA-MB-435 cell lines, we identified 8 genes with different copy numbers, including dystroglycan, which is located in the p12-21.2 area of chromosome 3. In conclusion, alterations in the level of the full-length variant of hTERT showed different gene expression profiles and genomic copy number changes in breast cancer, which require further study into their cause-and-effect relationship.

UR - http://www.scopus.com/inward/record.url?scp=33746406891&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746406891&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 749

EP - 755

JO - Oncology Reports

JF - Oncology Reports

SN - 1021-335X

IS - 4

ER -