Alteration of Inflammatory Mediators in the Upper and Lower Airways under Chronic Intermittent Hypoxia: Preliminary Animal Study

Eun Jung Lee, Woon Heo, Joo Young Kim, Hyungchul Kim, Min Jung Kang, Bo Ra Kim, Ji Hyun Kim, Do Yang Park, Chang Hoon Kim, Joo Heon Yoon, Hyung Ju Cho

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Abstract

Purpose. We hypothesized that CIH may affect the upper airway immune system and aimed to verify whether CIH can induce airway inflammation in a murine obstructive sleep apnea (OSA) model. Methods. C57BL6 male mice were exposed to intermittent hypoxia (CIH group; 5 21% FiO 2 , 120 sec cycles, 12 h/d, n=6) or room air (Sham group, n=6) for up to 4 weeks in identical chambers. Nasal and lung tissues and lavage fluid were collected and analyzed by multiplex assay. Lung lavage fluid was also utilized for FACS analysis to determine eosinophil count. Results. We determined the protein level of 24 different cytokines, chemokines, and inflammatory mediators. Among various cytokines, levels of IL-1, IL-1β, IL-4, IL-6, and IL-13 were significantly elevated in nose or lung tissue from the CIH group. In addition, MCP-1 and periostin were elevated in nose and lung tissue and lavage fluid from the CIH group. Conclusions. CIH for 4 weeks altered the levels of inflammatory mediators in both the nose and lungs of mouse model. We suggest that the airway immune system may be deteriorated by CIH and allergic inflammation in the upper or lower airway could be worsened by sleep apnea.

Original languageEnglish
Article number4327237
JournalMediators of Inflammation
Volume2017
DOIs
Publication statusPublished - 2017 Jan 1

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All Science Journal Classification (ASJC) codes

  • Immunology
  • Cell Biology

Cite this

Lee, E. J., Heo, W., Kim, J. Y., Kim, H., Kang, M. J., Kim, B. R., Kim, J. H., Park, D. Y., Kim, C. H., Yoon, J. H., & Cho, H. J. (2017). Alteration of Inflammatory Mediators in the Upper and Lower Airways under Chronic Intermittent Hypoxia: Preliminary Animal Study. Mediators of Inflammation, 2017, [4327237]. https://doi.org/10.1155/2017/4327237