Purpose. We hypothesized that CIH may affect the upper airway immune system and aimed to verify whether CIH can induce airway inflammation in a murine obstructive sleep apnea (OSA) model. Methods. C57BL6 male mice were exposed to intermittent hypoxia (CIH group; 5 21% FiO2, 120 sec cycles, 12 h/d, n=6) or room air (Sham group, n=6) for up to 4 weeks in identical chambers. Nasal and lung tissues and lavage fluid were collected and analyzed by multiplex assay. Lung lavage fluid was also utilized for FACS analysis to determine eosinophil count. Results. We determined the protein level of 24 different cytokines, chemokines, and inflammatory mediators. Among various cytokines, levels of IL-1, IL-1β, IL-4, IL-6, and IL-13 were significantly elevated in nose or lung tissue from the CIH group. In addition, MCP-1 and periostin were elevated in nose and lung tissue and lavage fluid from the CIH group. Conclusions. CIH for 4 weeks altered the levels of inflammatory mediators in both the nose and lungs of mouse model. We suggest that the airway immune system may be deteriorated by CIH and allergic inflammation in the upper or lower airway could be worsened by sleep apnea.
Bibliographical noteFunding Information:
This research was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1D1A1A02062156 and NRF-2013R1A1A 1010151) to Hyung-Ju Cho. This study was supported by a faculty research grant from Yonsei University College of Medicine for 2016 (6-2016-0061) and a new faculty research seed money grant from Yonsei University College of Medicine for 2017 (2017-32-0047). This research was also supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2016M3A9D5A01952414) awarded to Chang-Hoon Kim.
© 2017 Eun Jung Lee et al.
All Science Journal Classification (ASJC) codes
- Cell Biology