Alteration of payload in extracellular vesicles by crosstalk with mesenchymal stem cells from different origin

Dong Jun Park; Jeong Eun Park; Tae Hoon Kong; Young Joon Seo

doi:10.1186/s12951-021-00890-9

Alteration of payload in extracellular vesicles by crosstalk with mesenchymal stem cells from different origin

Dong Jun Park, Jeong Eun Park, Tae Hoon Kong, Young Joon Seo

Otorhinolaryngology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Background: The application of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) requires customized materials to target disease or cell damage. We hypothesized that EVs exert different inflammatory effects on one recipient cell, although stem cells of different origins in humans have similar payloads. Results: Here, the payload of EVs released by crosstalk between MSCs and human middle ear epithelial cells (HMEECs) extracted from adipose tissue, bone marrow and tonsils significantly increased the level of anti-inflammatory factors. EVs derived from the co-culture medium decreased TNF-α, COX-2, IL-1β, and IL-6 levels to approximately zero within 3 h in HMEECs. Expression of miR-638 and amyloid-β A4 precursor protein-binding family A member 2 was analyzed using microarrays and gene ontology analysis, respectively. Conclusions: In conclusion, stem cells of different origins have different payloads through crosstalk with recipient-specific cells. Inducing specific factors in EVs by co-culture with MSCs could be valuable in regenerative medicine. Graphical abstract: [Figure not available: see fulltext.].

Original language	English
Article number	148
Journal	Journal of Nanobiotechnology
Volume	19
Issue number	1
DOIs	https://doi.org/10.1186/s12951-021-00890-9
Publication status	Published - 2021 Dec

Bibliographical note

Funding Information:
This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) under Grant HI19C1334; and supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education (Grant: 2021R1I1A1A01040273). This work also supported by the National Research Foundation of Korea (NRF) funded by the Korean government (MIST) under Grant NRF-2020R1A2C1009789.

Funding Information:
This study was funded by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2020R1A2C1009789).

Publisher Copyright:
© 2021, The Author(s).

All Science Journal Classification (ASJC) codes

Bioengineering
Medicine (miscellaneous)
Molecular Medicine
Biomedical Engineering
Applied Microbiology and Biotechnology
Pharmaceutical Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1186/s12951-021-00890-9

Cite this

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abstract = "Background: The application of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) requires customized materials to target disease or cell damage. We hypothesized that EVs exert different inflammatory effects on one recipient cell, although stem cells of different origins in humans have similar payloads. Results: Here, the payload of EVs released by crosstalk between MSCs and human middle ear epithelial cells (HMEECs) extracted from adipose tissue, bone marrow and tonsils significantly increased the level of anti-inflammatory factors. EVs derived from the co-culture medium decreased TNF-α, COX-2, IL-1β, and IL-6 levels to approximately zero within 3 h in HMEECs. Expression of miR-638 and amyloid-β A4 precursor protein-binding family A member 2 was analyzed using microarrays and gene ontology analysis, respectively. Conclusions: In conclusion, stem cells of different origins have different payloads through crosstalk with recipient-specific cells. Inducing specific factors in EVs by co-culture with MSCs could be valuable in regenerative medicine. Graphical abstract: [Figure not available: see fulltext.].",

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note = "Funding Information: This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) under Grant HI19C1334; and supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education (Grant: 2021R1I1A1A01040273). This work also supported by the National Research Foundation of Korea (NRF) funded by the Korean government (MIST) under Grant NRF-2020R1A2C1009789. Funding Information: This study was funded by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2020R1A2C1009789). Publisher Copyright: {\textcopyright} 2021, The Author(s).",

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N1 - Funding Information: This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) under Grant HI19C1334; and supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education (Grant: 2021R1I1A1A01040273). This work also supported by the National Research Foundation of Korea (NRF) funded by the Korean government (MIST) under Grant NRF-2020R1A2C1009789. Funding Information: This study was funded by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2020R1A2C1009789). Publisher Copyright: © 2021, The Author(s).

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N2 - Background: The application of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) requires customized materials to target disease or cell damage. We hypothesized that EVs exert different inflammatory effects on one recipient cell, although stem cells of different origins in humans have similar payloads. Results: Here, the payload of EVs released by crosstalk between MSCs and human middle ear epithelial cells (HMEECs) extracted from adipose tissue, bone marrow and tonsils significantly increased the level of anti-inflammatory factors. EVs derived from the co-culture medium decreased TNF-α, COX-2, IL-1β, and IL-6 levels to approximately zero within 3 h in HMEECs. Expression of miR-638 and amyloid-β A4 precursor protein-binding family A member 2 was analyzed using microarrays and gene ontology analysis, respectively. Conclusions: In conclusion, stem cells of different origins have different payloads through crosstalk with recipient-specific cells. Inducing specific factors in EVs by co-culture with MSCs could be valuable in regenerative medicine. Graphical abstract: [Figure not available: see fulltext.].

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Alteration of payload in extracellular vesicles by crosstalk with mesenchymal stem cells from different origin

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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