Background: The application of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) requires customized materials to target disease or cell damage. We hypothesized that EVs exert different inflammatory effects on one recipient cell, although stem cells of different origins in humans have similar payloads. Results: Here, the payload of EVs released by crosstalk between MSCs and human middle ear epithelial cells (HMEECs) extracted from adipose tissue, bone marrow and tonsils significantly increased the level of anti-inflammatory factors. EVs derived from the co-culture medium decreased TNF-α, COX-2, IL-1β, and IL-6 levels to approximately zero within 3 h in HMEECs. Expression of miR-638 and amyloid-β A4 precursor protein-binding family A member 2 was analyzed using microarrays and gene ontology analysis, respectively. Conclusions: In conclusion, stem cells of different origins have different payloads through crosstalk with recipient-specific cells. Inducing specific factors in EVs by co-culture with MSCs could be valuable in regenerative medicine. Graphical abstract: [Figure not available: see fulltext.].
|Journal||Journal of Nanobiotechnology|
|Publication status||Published - 2021 Dec|
Bibliographical noteFunding Information:
This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) under Grant HI19C1334; and supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education (Grant: 2021R1I1A1A01040273). This work also supported by the National Research Foundation of Korea (NRF) funded by the Korean government (MIST) under Grant NRF-2020R1A2C1009789.
This study was funded by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2020R1A2C1009789).
© 2021, The Author(s).
All Science Journal Classification (ASJC) codes
- Medicine (miscellaneous)
- Molecular Medicine
- Biomedical Engineering
- Applied Microbiology and Biotechnology
- Pharmaceutical Science