Alterations of protein expression in macrophages in response to Candida albicans infection

Yu Kyong Shin, Ki Young Kim, Young Ki Paik

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Although macrophages are an important first line of cellular defense, they are unable to effectively kill phagocytosed C. albicans. To determine the physiological basis of this inability, we investigated the alterations of macrophage proteins caused by C. albicans infection. Since the formation of C. albicans hyphae caused cell death, proteins were prepared 3 h after infection and examined by two-dimensional gel electrophoresis (2-DE). The most prominent changes were in glycolytic enzymes, which could have caused energy depletion of the infected cells. Also changed were proteins involved in maintenance of cellular integrity and NO production. Treatment of the macrophages with either cytochalasin D or taxol did not alter their inability to kill C. albicans. Our results indicate that multiple factors contribute to cell death as the pathogenic form of C. albicans becomes fully active inside macrophage cells.

Original languageEnglish
Pages (from-to)271-279
Number of pages9
JournalMolecules and cells
Volume20
Issue number2
Publication statusPublished - 2005 Dec 1

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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