Abstract
Although macrophages are an important first line of cellular defense, they are unable to effectively kill phagocytosed C. albicans. To determine the physiological basis of this inability, we investigated the alterations of macrophage proteins caused by C. albicans infection. Since the formation of C. albicans hyphae caused cell death, proteins were prepared 3 h after infection and examined by two-dimensional gel electrophoresis (2-DE). The most prominent changes were in glycolytic enzymes, which could have caused energy depletion of the infected cells. Also changed were proteins involved in maintenance of cellular integrity and NO production. Treatment of the macrophages with either cytochalasin D or taxol did not alter their inability to kill C. albicans. Our results indicate that multiple factors contribute to cell death as the pathogenic form of C. albicans becomes fully active inside macrophage cells.
| Original language | English |
|---|---|
| Pages (from-to) | 271-279 |
| Number of pages | 9 |
| Journal | Molecules and cells |
| Volume | 20 |
| Issue number | 2 |
| Publication status | Published - 2005 Dec 1 |
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- Medicine(all)
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Alterations of protein expression in macrophages in response to Candida albicans infection. / Shin, Yu Kyong; Kim, Ki Young; Paik, Young-Ki.
In: Molecules and cells, Vol. 20, No. 2, 01.12.2005, p. 271-279.Research output: Contribution to journal › Article
TY - JOUR
T1 - Alterations of protein expression in macrophages in response to Candida albicans infection
AU - Shin, Yu Kyong
AU - Kim, Ki Young
AU - Paik, Young-Ki
PY - 2005/12/1
Y1 - 2005/12/1
N2 - Although macrophages are an important first line of cellular defense, they are unable to effectively kill phagocytosed C. albicans. To determine the physiological basis of this inability, we investigated the alterations of macrophage proteins caused by C. albicans infection. Since the formation of C. albicans hyphae caused cell death, proteins were prepared 3 h after infection and examined by two-dimensional gel electrophoresis (2-DE). The most prominent changes were in glycolytic enzymes, which could have caused energy depletion of the infected cells. Also changed were proteins involved in maintenance of cellular integrity and NO production. Treatment of the macrophages with either cytochalasin D or taxol did not alter their inability to kill C. albicans. Our results indicate that multiple factors contribute to cell death as the pathogenic form of C. albicans becomes fully active inside macrophage cells.
AB - Although macrophages are an important first line of cellular defense, they are unable to effectively kill phagocytosed C. albicans. To determine the physiological basis of this inability, we investigated the alterations of macrophage proteins caused by C. albicans infection. Since the formation of C. albicans hyphae caused cell death, proteins were prepared 3 h after infection and examined by two-dimensional gel electrophoresis (2-DE). The most prominent changes were in glycolytic enzymes, which could have caused energy depletion of the infected cells. Also changed were proteins involved in maintenance of cellular integrity and NO production. Treatment of the macrophages with either cytochalasin D or taxol did not alter their inability to kill C. albicans. Our results indicate that multiple factors contribute to cell death as the pathogenic form of C. albicans becomes fully active inside macrophage cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=33645235162&partnerID=8YFLogxK
M3 - Article
C2 - 16267403
AN - SCOPUS:33645235162
VL - 20
SP - 271
EP - 279
JO - Molecules and Cells
JF - Molecules and Cells
SN - 1016-8478
IS - 2
ER -