Altered epidermal lipid layers induced by long-term exposure to suberythemal-dose ultraviolet

Hana Bak, Seung phil Hong, Se Kyoo Jeong, Eung Ho Choi, Sang E. Lee, Seung Hun Lee, Sung Ku Ahn

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background Although several studies have reported on the biological effects of ultraviolet (UV) radiation, there have been only a few reports on the changes in epidermal lipids following long-term UV irradiation at suberythemal dose (SED), to which people are usually exposed during their lifetime. Objectives To investigate the changes of epidermal lipid properties after long-term UV radiation with SED. Materials and methods Hairless mice were irradiated three times weekly for 15weeks at an SED of UV (UVB: 20mJ/cm2; UVA: 14J/cm2). Every three weeks, transepidermal water loss (TEWL) was measured by a Tewameter. The morphological alterations of stratum corneum (SC) lipid lamellae were examined by electron microscopy (EM). Activities of three key enzymes for mRNA of serine palmitoyl transferase, fatty acid synthase, and HMG CoA reductase were analyzed with real time reverse transcriptase-polymerase chain reaction. We also measured the amount of ceramide, cholesterol sulfate, and free fatty acid in the SC by high-performance thin-layer chromatography with exposed times. Results The SED UV-irradiated group showed increased TEWL after 12weeks. Following the irradiation period, EM revealed incomplete and separated lamellae at SC intercellular space. mRNA of three key enzymes was increased until six weeks of UV irradiation and decreased thereafter. However, three major lipid amounts gradually decreased throughout the exposed period, with a notable decrease in ceramide. Conclusions Long-term UV irradiation even with SED influences skin barrier function and structure with prominent ceramide decrease in SC intercellular lipid.

Original languageEnglish
Pages (from-to)832-837
Number of pages6
JournalInternational Journal of Dermatology
Volume50
Issue number7
DOIs
Publication statusPublished - 2011 Jul 1

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Cornea
Ceramides
Lipids
Electron Microscopy
Hydroxymethylglutaryl CoA Reductases
Hairless Mouse
Fatty Acid Synthases
Messenger RNA
Water
Radiation Effects
Extracellular Space
Enzymes
Thin Layer Chromatography
Transferases
Reverse Transcriptase Polymerase Chain Reaction
Nonesterified Fatty Acids
Serine
Real-Time Polymerase Chain Reaction
Radiation
Skin

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Bak, Hana ; Hong, Seung phil ; Jeong, Se Kyoo ; Choi, Eung Ho ; Lee, Sang E. ; Lee, Seung Hun ; Ahn, Sung Ku. / Altered epidermal lipid layers induced by long-term exposure to suberythemal-dose ultraviolet. In: International Journal of Dermatology. 2011 ; Vol. 50, No. 7. pp. 832-837.
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abstract = "Background Although several studies have reported on the biological effects of ultraviolet (UV) radiation, there have been only a few reports on the changes in epidermal lipids following long-term UV irradiation at suberythemal dose (SED), to which people are usually exposed during their lifetime. Objectives To investigate the changes of epidermal lipid properties after long-term UV radiation with SED. Materials and methods Hairless mice were irradiated three times weekly for 15weeks at an SED of UV (UVB: 20mJ/cm2; UVA: 14J/cm2). Every three weeks, transepidermal water loss (TEWL) was measured by a Tewameter. The morphological alterations of stratum corneum (SC) lipid lamellae were examined by electron microscopy (EM). Activities of three key enzymes for mRNA of serine palmitoyl transferase, fatty acid synthase, and HMG CoA reductase were analyzed with real time reverse transcriptase-polymerase chain reaction. We also measured the amount of ceramide, cholesterol sulfate, and free fatty acid in the SC by high-performance thin-layer chromatography with exposed times. Results The SED UV-irradiated group showed increased TEWL after 12weeks. Following the irradiation period, EM revealed incomplete and separated lamellae at SC intercellular space. mRNA of three key enzymes was increased until six weeks of UV irradiation and decreased thereafter. However, three major lipid amounts gradually decreased throughout the exposed period, with a notable decrease in ceramide. Conclusions Long-term UV irradiation even with SED influences skin barrier function and structure with prominent ceramide decrease in SC intercellular lipid.",
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Altered epidermal lipid layers induced by long-term exposure to suberythemal-dose ultraviolet. / Bak, Hana; Hong, Seung phil; Jeong, Se Kyoo; Choi, Eung Ho; Lee, Sang E.; Lee, Seung Hun; Ahn, Sung Ku.

In: International Journal of Dermatology, Vol. 50, No. 7, 01.07.2011, p. 832-837.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Altered epidermal lipid layers induced by long-term exposure to suberythemal-dose ultraviolet

AU - Bak, Hana

AU - Hong, Seung phil

AU - Jeong, Se Kyoo

AU - Choi, Eung Ho

AU - Lee, Sang E.

AU - Lee, Seung Hun

AU - Ahn, Sung Ku

PY - 2011/7/1

Y1 - 2011/7/1

N2 - Background Although several studies have reported on the biological effects of ultraviolet (UV) radiation, there have been only a few reports on the changes in epidermal lipids following long-term UV irradiation at suberythemal dose (SED), to which people are usually exposed during their lifetime. Objectives To investigate the changes of epidermal lipid properties after long-term UV radiation with SED. Materials and methods Hairless mice were irradiated three times weekly for 15weeks at an SED of UV (UVB: 20mJ/cm2; UVA: 14J/cm2). Every three weeks, transepidermal water loss (TEWL) was measured by a Tewameter. The morphological alterations of stratum corneum (SC) lipid lamellae were examined by electron microscopy (EM). Activities of three key enzymes for mRNA of serine palmitoyl transferase, fatty acid synthase, and HMG CoA reductase were analyzed with real time reverse transcriptase-polymerase chain reaction. We also measured the amount of ceramide, cholesterol sulfate, and free fatty acid in the SC by high-performance thin-layer chromatography with exposed times. Results The SED UV-irradiated group showed increased TEWL after 12weeks. Following the irradiation period, EM revealed incomplete and separated lamellae at SC intercellular space. mRNA of three key enzymes was increased until six weeks of UV irradiation and decreased thereafter. However, three major lipid amounts gradually decreased throughout the exposed period, with a notable decrease in ceramide. Conclusions Long-term UV irradiation even with SED influences skin barrier function and structure with prominent ceramide decrease in SC intercellular lipid.

AB - Background Although several studies have reported on the biological effects of ultraviolet (UV) radiation, there have been only a few reports on the changes in epidermal lipids following long-term UV irradiation at suberythemal dose (SED), to which people are usually exposed during their lifetime. Objectives To investigate the changes of epidermal lipid properties after long-term UV radiation with SED. Materials and methods Hairless mice were irradiated three times weekly for 15weeks at an SED of UV (UVB: 20mJ/cm2; UVA: 14J/cm2). Every three weeks, transepidermal water loss (TEWL) was measured by a Tewameter. The morphological alterations of stratum corneum (SC) lipid lamellae were examined by electron microscopy (EM). Activities of three key enzymes for mRNA of serine palmitoyl transferase, fatty acid synthase, and HMG CoA reductase were analyzed with real time reverse transcriptase-polymerase chain reaction. We also measured the amount of ceramide, cholesterol sulfate, and free fatty acid in the SC by high-performance thin-layer chromatography with exposed times. Results The SED UV-irradiated group showed increased TEWL after 12weeks. Following the irradiation period, EM revealed incomplete and separated lamellae at SC intercellular space. mRNA of three key enzymes was increased until six weeks of UV irradiation and decreased thereafter. However, three major lipid amounts gradually decreased throughout the exposed period, with a notable decrease in ceramide. Conclusions Long-term UV irradiation even with SED influences skin barrier function and structure with prominent ceramide decrease in SC intercellular lipid.

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