Abstract
To clarify the involvement of phospholipase D (PLD) in the mechanism underlying genetically-induced hypertension, we investigated the activity and expression levels of PLD in tissues taken from spontaneously hypertensive rats (SHR), and their normotensive controls, Wistar-Kyoto rats (WKY). The ADP-ribosylation factor 3 (ARF3)-dependent PLD activity and protein levels of PLD1 from SHR increased significantly in the brain and liver, but not in the heart and kidney, compared to those of WKY. The activity and expression of PLD were the same between the homogenated whole kidneys of the two strains; however, there were topographical differences in the expression and activity of PLD between the kidneys of the two strains. The activity and expression level of PLD gradually increased from the cortex to the inner medulla of WKY. The enzyme activity, and amount of PLD in the inner stripe of the outer medulla and in the inner medulla, was significantly lower in SHR than in WKY. Taken together, these results suggest that the distinctly distributed patterns of PLD in the kidney may be associated with differential signal transduction pathways that are involved in hypertension in conjunction with an increase of PLD activity in the brain and liver.
| Original language | English |
|---|---|
| Pages (from-to) | 386-391 |
| Number of pages | 6 |
| Journal | Molecules and cells |
| Volume | 11 |
| Issue number | 3 |
| Publication status | Published - 2001 Jun 30 |
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All Science Journal Classification (ASJC) codes
- Cell Biology
- Genetics
- Molecular Biology
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Altered expression of phospholipase D1 in spontaneously hypertensive rats. / Min, Do Sik; Lee, Kweon Haeng; Chang, Jong Soo; Ahn, Bong Hyun; Rhie, Duck Joo; Yoon, Shin Hee; Hahn, Sang June; Kim, Myung Suk; Jo, Yang Hyeok.
In: Molecules and cells, Vol. 11, No. 3, 30.06.2001, p. 386-391.Research output: Contribution to journal › Article
TY - JOUR
T1 - Altered expression of phospholipase D1 in spontaneously hypertensive rats
AU - Min, Do Sik
AU - Lee, Kweon Haeng
AU - Chang, Jong Soo
AU - Ahn, Bong Hyun
AU - Rhie, Duck Joo
AU - Yoon, Shin Hee
AU - Hahn, Sang June
AU - Kim, Myung Suk
AU - Jo, Yang Hyeok
PY - 2001/6/30
Y1 - 2001/6/30
N2 - To clarify the involvement of phospholipase D (PLD) in the mechanism underlying genetically-induced hypertension, we investigated the activity and expression levels of PLD in tissues taken from spontaneously hypertensive rats (SHR), and their normotensive controls, Wistar-Kyoto rats (WKY). The ADP-ribosylation factor 3 (ARF3)-dependent PLD activity and protein levels of PLD1 from SHR increased significantly in the brain and liver, but not in the heart and kidney, compared to those of WKY. The activity and expression of PLD were the same between the homogenated whole kidneys of the two strains; however, there were topographical differences in the expression and activity of PLD between the kidneys of the two strains. The activity and expression level of PLD gradually increased from the cortex to the inner medulla of WKY. The enzyme activity, and amount of PLD in the inner stripe of the outer medulla and in the inner medulla, was significantly lower in SHR than in WKY. Taken together, these results suggest that the distinctly distributed patterns of PLD in the kidney may be associated with differential signal transduction pathways that are involved in hypertension in conjunction with an increase of PLD activity in the brain and liver.
AB - To clarify the involvement of phospholipase D (PLD) in the mechanism underlying genetically-induced hypertension, we investigated the activity and expression levels of PLD in tissues taken from spontaneously hypertensive rats (SHR), and their normotensive controls, Wistar-Kyoto rats (WKY). The ADP-ribosylation factor 3 (ARF3)-dependent PLD activity and protein levels of PLD1 from SHR increased significantly in the brain and liver, but not in the heart and kidney, compared to those of WKY. The activity and expression of PLD were the same between the homogenated whole kidneys of the two strains; however, there were topographical differences in the expression and activity of PLD between the kidneys of the two strains. The activity and expression level of PLD gradually increased from the cortex to the inner medulla of WKY. The enzyme activity, and amount of PLD in the inner stripe of the outer medulla and in the inner medulla, was significantly lower in SHR than in WKY. Taken together, these results suggest that the distinctly distributed patterns of PLD in the kidney may be associated with differential signal transduction pathways that are involved in hypertension in conjunction with an increase of PLD activity in the brain and liver.
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UR - http://www.scopus.com/inward/citedby.url?scp=0035973568&partnerID=8YFLogxK
M3 - Article
C2 - 11459230
AN - SCOPUS:0035973568
VL - 11
SP - 386
EP - 391
JO - Molecules and Cells
JF - Molecules and Cells
SN - 1016-8478
IS - 3
ER -