Altered expression of phospholipase D1 in spontaneously hypertensive rats

Do Sik Min, Kweon Haeng Lee, Jong Soo Chang, Bong Hyun Ahn, Duck Joo Rhie, Shin Hee Yoon, Sang June Hahn, Myung Suk Kim, Yang Hyeok Jo

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To clarify the involvement of phospholipase D (PLD) in the mechanism underlying genetically-induced hypertension, we investigated the activity and expression levels of PLD in tissues taken from spontaneously hypertensive rats (SHR), and their normotensive controls, Wistar-Kyoto rats (WKY). The ADP-ribosylation factor 3 (ARF3)-dependent PLD activity and protein levels of PLD1 from SHR increased significantly in the brain and liver, but not in the heart and kidney, compared to those of WKY. The activity and expression of PLD were the same between the homogenated whole kidneys of the two strains; however, there were topographical differences in the expression and activity of PLD between the kidneys of the two strains. The activity and expression level of PLD gradually increased from the cortex to the inner medulla of WKY. The enzyme activity, and amount of PLD in the inner stripe of the outer medulla and in the inner medulla, was significantly lower in SHR than in WKY. Taken together, these results suggest that the distinctly distributed patterns of PLD in the kidney may be associated with differential signal transduction pathways that are involved in hypertension in conjunction with an increase of PLD activity in the brain and liver.

Original languageEnglish
Pages (from-to)386-391
Number of pages6
JournalMolecules and cells
Volume11
Issue number3
Publication statusPublished - 2001 Jun 30

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Phospholipase D
Inbred SHR Rats
Inbred WKY Rats
Kidney
ADP-Ribosylation Factors
Hypertension
phospholipase D1
Liver
Brain
Signal Transduction

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Min, D. S., Lee, K. H., Chang, J. S., Ahn, B. H., Rhie, D. J., Yoon, S. H., ... Jo, Y. H. (2001). Altered expression of phospholipase D1 in spontaneously hypertensive rats. Molecules and cells, 11(3), 386-391.
Min, Do Sik ; Lee, Kweon Haeng ; Chang, Jong Soo ; Ahn, Bong Hyun ; Rhie, Duck Joo ; Yoon, Shin Hee ; Hahn, Sang June ; Kim, Myung Suk ; Jo, Yang Hyeok. / Altered expression of phospholipase D1 in spontaneously hypertensive rats. In: Molecules and cells. 2001 ; Vol. 11, No. 3. pp. 386-391.
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Min, DS, Lee, KH, Chang, JS, Ahn, BH, Rhie, DJ, Yoon, SH, Hahn, SJ, Kim, MS & Jo, YH 2001, 'Altered expression of phospholipase D1 in spontaneously hypertensive rats', Molecules and cells, vol. 11, no. 3, pp. 386-391.

Altered expression of phospholipase D1 in spontaneously hypertensive rats. / Min, Do Sik; Lee, Kweon Haeng; Chang, Jong Soo; Ahn, Bong Hyun; Rhie, Duck Joo; Yoon, Shin Hee; Hahn, Sang June; Kim, Myung Suk; Jo, Yang Hyeok.

In: Molecules and cells, Vol. 11, No. 3, 30.06.2001, p. 386-391.

Research output: Contribution to journalArticle

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Min DS, Lee KH, Chang JS, Ahn BH, Rhie DJ, Yoon SH et al. Altered expression of phospholipase D1 in spontaneously hypertensive rats. Molecules and cells. 2001 Jun 30;11(3):386-391.