Altered gene expression in cerulein-stimulated pancreatic acinar cells: Pathologic mechanism of acute pancreatitis

Ji Hoo Yu, Joo Weon Lim, Hyeyoung Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Acute pancreatitis is a multifactorial disease associated with the premature activation of digestive enzymes. The genes expressed in pancreatic acinar cells determine the severity of the disease. The present study determined the differentially expressed genes in pancreatic acinar cells treated with cerulein as an in vitro model of acute pancreatitis. Pancreatic acinar AR42J cells were stimulated with 10-8 M cerulein for 4 h, and genes with altered expression were identified using a cDNA microarray for 4,000 rat genes and validated by real-time PCR. These genes showed a 2.5-fold or higher increase with cerulein: lithostatin, guanylate cyclase, myosin light chain kinase 2, cathepsin C, progestin-induced protein, and pancreatic trypsin 2. Stathin 1 and ribosomal protein S13 showed a 2.5-fold or higher decreases in expression. Real-time PCR analysis showed time-dependent alterations of these genes. Using commercially available antibodies specific for guanylate cyclase, myosin light chain kinase 2, and cathepsin C, a time-dependent increase in these proteins were observed by Western blotting. Thus, disturbances in proliferation, differentiation, cytoskeleton arrangement, enzyme activity, and secretion may be underlying mechanisms of acute pancreatitis.

Original languageEnglish
Pages (from-to)409-416
Number of pages8
JournalKorean Journal of Physiology and Pharmacology
Volume13
Issue number6
DOIs
Publication statusPublished - 2009 Dec

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pharmacology

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