Altered hydroxylation of estrogen in patients with postmenopausal osteopenia

Sung Kil Lim, Young Jun Won, Ji Hyun Lee, Suk Ho Kwon, Eun Jig Lee, Kyung Rae Kim, Hyun Chul Lee, Kap Bum Huh, Bong Chul Chung

Research output: Contribution to journalArticle

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Abstract

To study the possible contributions of the differences in estrogen metabolism to bone mass in postmenopausal osteopenia, spinal and femoral bone mineral densities (BMD) were measured, and 18 urinary metabolites of estrogen were analyzed by a gas chromatography-mass spectrometry assay system in 59 postmenopausal women (5-10 yr after menopause). The BMD of the spine and femoral neck showed positive correlations with body weight, height, and body mass index as we expected. Compared to nonosteopenic subjects, there were no significant differences in serum estrone (E1) and estradiol (E2) levels in patients with osteopenia. However, the urinary 16α-hydroxyestrone [16α- (OH)E1] level was significantly lower in patients with spinal osteopenia (P < 0.001). Among the 18 urinary metabolites of estrogen, the 16α-(OH)E1 and 16α-(OH)E1/2-hydroxyestrone [2-(OH)E1) ratio showed positive correlations with spinal BMD (P < 0.05), whereas 2-(OH)E2 showed a negative correlation with femoral neck BMD (P < 0.05). The urinary 16α-(OH)E1 level also revealed a positive correlation with the age-matched z score of BMD in the spine (P < 0.05). In multiple stepwise regression analysis, weight, 16α(OH)E1, interaction between 16α-(OH)E1 and 2-(OH)E2, 2-(OH)E2, and years after menopause were statistically significant for spinal BMD (r2 = 0.4968). For femoral neck BMD and weight, 16α-(OH)E1 and 2-(OH)E2 were the independent determinants (r2 = 0.3369). In conclusion, the activity of estrogen 16α-hydroxylase was decreased and/or the activity of estrogen 2- hydroxylase was enhanced in postmenopausal osteopenia. We speculated that these derangements may serve as contributing factors for the acceleration of hone loss in postmenopausal osteoporosis.

Original languageEnglish
Pages (from-to)1001-1006
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume82
Issue number4
DOIs
Publication statusPublished - 1997 Jan 1

Fingerprint

Hydroxylation
Metabolic Bone Diseases
Bone Density
Bone
Estrogens
Minerals
Femur Neck
Menopause
Metabolites
Spine
Weights and Measures
Postmenopausal Osteoporosis
Body Height
Estrone
Mixed Function Oxygenases
Thigh
Gas Chromatography-Mass Spectrometry
Metabolism
Estradiol
Regression analysis

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Lim, Sung Kil ; Won, Young Jun ; Lee, Ji Hyun ; Kwon, Suk Ho ; Lee, Eun Jig ; Kim, Kyung Rae ; Lee, Hyun Chul ; Huh, Kap Bum ; Chung, Bong Chul. / Altered hydroxylation of estrogen in patients with postmenopausal osteopenia. In: Journal of Clinical Endocrinology and Metabolism. 1997 ; Vol. 82, No. 4. pp. 1001-1006.
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Altered hydroxylation of estrogen in patients with postmenopausal osteopenia. / Lim, Sung Kil; Won, Young Jun; Lee, Ji Hyun; Kwon, Suk Ho; Lee, Eun Jig; Kim, Kyung Rae; Lee, Hyun Chul; Huh, Kap Bum; Chung, Bong Chul.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 82, No. 4, 01.01.1997, p. 1001-1006.

Research output: Contribution to journalArticle

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T1 - Altered hydroxylation of estrogen in patients with postmenopausal osteopenia

AU - Lim, Sung Kil

AU - Won, Young Jun

AU - Lee, Ji Hyun

AU - Kwon, Suk Ho

AU - Lee, Eun Jig

AU - Kim, Kyung Rae

AU - Lee, Hyun Chul

AU - Huh, Kap Bum

AU - Chung, Bong Chul

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N2 - To study the possible contributions of the differences in estrogen metabolism to bone mass in postmenopausal osteopenia, spinal and femoral bone mineral densities (BMD) were measured, and 18 urinary metabolites of estrogen were analyzed by a gas chromatography-mass spectrometry assay system in 59 postmenopausal women (5-10 yr after menopause). The BMD of the spine and femoral neck showed positive correlations with body weight, height, and body mass index as we expected. Compared to nonosteopenic subjects, there were no significant differences in serum estrone (E1) and estradiol (E2) levels in patients with osteopenia. However, the urinary 16α-hydroxyestrone [16α- (OH)E1] level was significantly lower in patients with spinal osteopenia (P < 0.001). Among the 18 urinary metabolites of estrogen, the 16α-(OH)E1 and 16α-(OH)E1/2-hydroxyestrone [2-(OH)E1) ratio showed positive correlations with spinal BMD (P < 0.05), whereas 2-(OH)E2 showed a negative correlation with femoral neck BMD (P < 0.05). The urinary 16α-(OH)E1 level also revealed a positive correlation with the age-matched z score of BMD in the spine (P < 0.05). In multiple stepwise regression analysis, weight, 16α(OH)E1, interaction between 16α-(OH)E1 and 2-(OH)E2, 2-(OH)E2, and years after menopause were statistically significant for spinal BMD (r2 = 0.4968). For femoral neck BMD and weight, 16α-(OH)E1 and 2-(OH)E2 were the independent determinants (r2 = 0.3369). In conclusion, the activity of estrogen 16α-hydroxylase was decreased and/or the activity of estrogen 2- hydroxylase was enhanced in postmenopausal osteopenia. We speculated that these derangements may serve as contributing factors for the acceleration of hone loss in postmenopausal osteoporosis.

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