Altered vimentin protein expression in human dermal microvascular endothelial cells after ultraviolet or intense pulsed light treatment

Jung U. Shin, Won Jai Lee, Sang Ho Oh, Do Young Kim, Dae Suk Kim, Inhee Jung, Ju Hee Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background Endothelial cell senescence is closely related to tissue aging and age-related vascular disease. Detailed pathophysiology and essential biomarkers of skin aging are not well known. A recent report suggests that advanced glycosylation end products, especially Nε- (carboxymethyl)lysine (CML) modification of vimentin, accelerate the aging process. Objectives To identify protein biomarkers of aging in skin. Materials and Methods Proteomics analysis was performed to identify differentially expressed proteins in human dermal microvascular endothelial cells (HDMEC) treated with ultraviolet (UV) or intense pulsed light (IPL). Proteome maps of UV-treated, IPL-treated, and untreated HDMEC were constructed, with identification of altered protein spots by matrix-assisted laser desorption/ionization mass spectrometry. Differential expression and glycation modification of vimentin were found by this approach and further examined by fluorescence-activated cell sorting. Results Twenty-two differentially expressed protein spots were identified. Among them, vimentin was specifically up-regulated in UV-treated HDMECs. On the other hand, it was down-regulated after IPL. Increased expression of CML-vimentin in HDMEC during culture (Passage 6 vs. 12) was noted, and this effect was reversed by IPL treatment. Conclusion Vimentin and CML should be useful markers for cell senescence, as well as for evaluating the level of aging. Also, targeting increased vimentin expression and its advanced glycation end products could present a target for the treatment of skin aging.

Original languageEnglish
Pages (from-to)431-438
Number of pages8
JournalLasers in Surgery and Medicine
Volume46
Issue number5
DOIs
Publication statusPublished - 2014 Jul

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Vimentin
Endothelial Cells
Skin Aging
Light
Skin
Advanced Glycosylation End Products
Proteins
Cell Aging
Biomarkers
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Proteome
Vascular Diseases
Proteomics
Flow Cytometry
Cell Culture Techniques
N(6)-carboxymethyllysine

All Science Journal Classification (ASJC) codes

  • Surgery
  • Dermatology

Cite this

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title = "Altered vimentin protein expression in human dermal microvascular endothelial cells after ultraviolet or intense pulsed light treatment",
abstract = "Background Endothelial cell senescence is closely related to tissue aging and age-related vascular disease. Detailed pathophysiology and essential biomarkers of skin aging are not well known. A recent report suggests that advanced glycosylation end products, especially Nε- (carboxymethyl)lysine (CML) modification of vimentin, accelerate the aging process. Objectives To identify protein biomarkers of aging in skin. Materials and Methods Proteomics analysis was performed to identify differentially expressed proteins in human dermal microvascular endothelial cells (HDMEC) treated with ultraviolet (UV) or intense pulsed light (IPL). Proteome maps of UV-treated, IPL-treated, and untreated HDMEC were constructed, with identification of altered protein spots by matrix-assisted laser desorption/ionization mass spectrometry. Differential expression and glycation modification of vimentin were found by this approach and further examined by fluorescence-activated cell sorting. Results Twenty-two differentially expressed protein spots were identified. Among them, vimentin was specifically up-regulated in UV-treated HDMECs. On the other hand, it was down-regulated after IPL. Increased expression of CML-vimentin in HDMEC during culture (Passage 6 vs. 12) was noted, and this effect was reversed by IPL treatment. Conclusion Vimentin and CML should be useful markers for cell senescence, as well as for evaluating the level of aging. Also, targeting increased vimentin expression and its advanced glycation end products could present a target for the treatment of skin aging.",
author = "Shin, {Jung U.} and Lee, {Won Jai} and Oh, {Sang Ho} and Kim, {Do Young} and Kim, {Dae Suk} and Inhee Jung and Lee, {Ju Hee}",
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Altered vimentin protein expression in human dermal microvascular endothelial cells after ultraviolet or intense pulsed light treatment. / Shin, Jung U.; Lee, Won Jai; Oh, Sang Ho; Kim, Do Young; Kim, Dae Suk; Jung, Inhee; Lee, Ju Hee.

In: Lasers in Surgery and Medicine, Vol. 46, No. 5, 07.2014, p. 431-438.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Altered vimentin protein expression in human dermal microvascular endothelial cells after ultraviolet or intense pulsed light treatment

AU - Shin, Jung U.

AU - Lee, Won Jai

AU - Oh, Sang Ho

AU - Kim, Do Young

AU - Kim, Dae Suk

AU - Jung, Inhee

AU - Lee, Ju Hee

PY - 2014/7

Y1 - 2014/7

N2 - Background Endothelial cell senescence is closely related to tissue aging and age-related vascular disease. Detailed pathophysiology and essential biomarkers of skin aging are not well known. A recent report suggests that advanced glycosylation end products, especially Nε- (carboxymethyl)lysine (CML) modification of vimentin, accelerate the aging process. Objectives To identify protein biomarkers of aging in skin. Materials and Methods Proteomics analysis was performed to identify differentially expressed proteins in human dermal microvascular endothelial cells (HDMEC) treated with ultraviolet (UV) or intense pulsed light (IPL). Proteome maps of UV-treated, IPL-treated, and untreated HDMEC were constructed, with identification of altered protein spots by matrix-assisted laser desorption/ionization mass spectrometry. Differential expression and glycation modification of vimentin were found by this approach and further examined by fluorescence-activated cell sorting. Results Twenty-two differentially expressed protein spots were identified. Among them, vimentin was specifically up-regulated in UV-treated HDMECs. On the other hand, it was down-regulated after IPL. Increased expression of CML-vimentin in HDMEC during culture (Passage 6 vs. 12) was noted, and this effect was reversed by IPL treatment. Conclusion Vimentin and CML should be useful markers for cell senescence, as well as for evaluating the level of aging. Also, targeting increased vimentin expression and its advanced glycation end products could present a target for the treatment of skin aging.

AB - Background Endothelial cell senescence is closely related to tissue aging and age-related vascular disease. Detailed pathophysiology and essential biomarkers of skin aging are not well known. A recent report suggests that advanced glycosylation end products, especially Nε- (carboxymethyl)lysine (CML) modification of vimentin, accelerate the aging process. Objectives To identify protein biomarkers of aging in skin. Materials and Methods Proteomics analysis was performed to identify differentially expressed proteins in human dermal microvascular endothelial cells (HDMEC) treated with ultraviolet (UV) or intense pulsed light (IPL). Proteome maps of UV-treated, IPL-treated, and untreated HDMEC were constructed, with identification of altered protein spots by matrix-assisted laser desorption/ionization mass spectrometry. Differential expression and glycation modification of vimentin were found by this approach and further examined by fluorescence-activated cell sorting. Results Twenty-two differentially expressed protein spots were identified. Among them, vimentin was specifically up-regulated in UV-treated HDMECs. On the other hand, it was down-regulated after IPL. Increased expression of CML-vimentin in HDMEC during culture (Passage 6 vs. 12) was noted, and this effect was reversed by IPL treatment. Conclusion Vimentin and CML should be useful markers for cell senescence, as well as for evaluating the level of aging. Also, targeting increased vimentin expression and its advanced glycation end products could present a target for the treatment of skin aging.

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JO - Lasers in Surgery and Medicine

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SN - 0196-8092

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